Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings

Publication Overview
TitleEvaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings
AuthorsZhang H, Xu W, Chen H, chen J, Chen X, Yang S
TypeJournal Article
Journal NamePlant Molecular Biology Reporter
Volume38
Year2020
Page(s)294-304
CitationZhang H, Xu W, Chen H, chen J, Chen X, Yang S. Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings. Plant Molecular Biology Reporter. 2020;38:294-304.

Abstract

Salinity is a major abiotic stress that limits productivity in yardlong bean. Identification of the quantitative trait loci (QTL) underlying salt tolerance is a prerequisite for successful marker-assisted selection when breeding for salt-tolerance. The objective of this study was to use a set of insertions and deletions (InDels) found in salt stress-responsive genes to construct a linkage map and identify reliable markers that can guide breeding efforts for novel, high-yield, salttolerant varieties of yardlong bean (Vigna unguiculata). A linkage map was constructed from 103 InDel markers in an F2 population consisting of 143 plants produced by crossing yardlong bean varieties Suzi 41 (salt tolerant) and Sujiang 1419 (salt sensitive). The markers were clustered across 9 chromosomes spanning 975.7 cM in total length, with an average distance between adjacent markers of 9.5 cM. Using this linkage map, we identified six major QTLs associated with control of salt tolerance-related traits on chromosomes (Chr.) 08, 09, and 11, which explained 9.9% ~ 72.7% of the phenotypic variation. Four major QTLs showed co-location in the region between VUIn584 and VUIn724 on Chr.09, associated with the traits of salt tolerance rating (STR), leaf chlorophyll content (SPAD), aerial biomass fresh weight (AFW), and root fresh weight (RFW). These QTLs provide a valuable reference for marker-assisted breeding, QTL fine mapping, and the cloning and characterization of salt-tolerance candidate genes. Keywords Genetic map . InDel marker . QTL analysis . Salt stress . Vigna unguiculata
Features
This publication contains information about 109 features:
Feature NameUniquenameType
VUIn699VUIn699genetic_marker
VUIn815VUIn815genetic_marker
VUIn842VUIn842genetic_marker
Saline sensitivityqSALTSN.Suzi41/Sujiang1419.LG09QTL
SPAD chlorophyll meter readingsqSPAD.Suzi41/Sujiang1419.LG09QTL
Shoot biomassqSHB.Suzi41/Sujiang1419.LG09QTL
Root fresh weightqRFWT.Suzi41/Sujiang1419.LG09QTL
Shoot dry weightqSTWT.Suzi41/Sujiang1419.LG11QTL
Root dry weightqRTWT.Suzi41/Sujiang1419.LG08QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Vigna-Salt_tolerance-Zhang-2020
Featuremaps
This publication contains information about 1 maps:
Map Name
Vigna-Suzi41/Sujiang1419-F2
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Suzi 41Suzi 41accession
Sujiang 1419Sujiang 1419accession
Suzi41/Sujiang1419-F2Suzi41/Sujiang1419-F2population
Properties
Additional details for this publication include:
Property NameValue
URLhttps://link.springer.com/article/10.1007/s11105-020-01194-2