Identification of Root Rot Resistance QTLs in Pea Using Fusarium solani f. sp. pisi-Responsive Differentially Expressed Genes.

Publication Overview
TitleIdentification of Root Rot Resistance QTLs in Pea Using Fusarium solani f. sp. pisi-Responsive Differentially Expressed Genes.
AuthorsWilliamson-Benavides BA, Sharpe RM, Nelson G, Bodah ET, Porter LD, Dhingra A
TypeJournal Article
Journal NameFrontiers in genetics
Volume12
Year2021
Page(s)629267
CitationWilliamson-Benavides BA, Sharpe RM, Nelson G, Bodah ET, Porter LD, Dhingra A. Identification of Root Rot Resistance QTLs in Pea Using Fusarium solani f. sp. pisi-Responsive Differentially Expressed Genes.. Frontiers in genetics. 2021; 12:629267.

Abstract

Pisum sativum (pea) yields in the United States have declined significantly over the last decades, predominantly due to susceptibility to root rot diseases. One of the main causal agents of root rot is the fungus Fusarium solani f. sp. pisi (Fsp), leading to yield losses ranging from 15 to 60%. Determining and subsequently incorporating the genetic basis for resistance in new cultivars offers one of the best solutions to control this pathogen; however, no green-seeded pea cultivars with complete resistance to Fsp have been identified. To date, only partial levels of resistance to Fsp has been identified among pea genotypes. SNPs mined from Fsp-responsive differentially expressed genes (DEGs) identified in a preceding study were utilized to identify QTLs associated with Fsp resistance using composite interval mapping in two recombinant inbred line (RIL) populations segregating for partial root rot resistance. A total of 769 DEGs with single nucleotide polymorphisms (SNPs) were identified, and the putative SNPs were evaluated for being polymorphic across four partially resistant and four susceptible P. sativum genotypes. The SNPs with validated polymorphisms were used to screen two RIL populations using two phenotypic criteria: root disease severity and plant height. One QTL, WB.Fsp-Ps 5.1 that mapped to chromosome 5 explained 14.8% of the variance with a confidence interval of 10.4 cM. The other four QTLs located on chromosomes 2, 3, and 5, explained 5.3-8.1% of the variance. The use of SNPs derived from Fsp-responsive DEGs for QTL mapping proved to be an efficient way to identify molecular markers associated with Fsp resistance in pea. These QTLs are potential candidates for marker-assisted selection and gene pyramiding to obtain high levels of partial resistance in pea cultivars to combat root rot caused by Fsp.

Features
This publication contains information about 752 features:
Feature NameUniquenameType
WSU21_54251WSU21_54251genetic_marker
WSU21_54774WSU21_54774genetic_marker
WSU21_55462WSU21_55462genetic_marker
WSU21_55799WSU21_55799genetic_marker
WSU21_56670WSU21_56670genetic_marker
WSU21_57284WSU21_57284genetic_marker
WSU21_57335WSU21_57335genetic_marker
WSU21_57579WSU21_57579genetic_marker
WSU21_58768WSU21_58768genetic_marker
WSU21_58801WSU21_58801genetic_marker
WSU21_59643WSU21_59643genetic_marker
WSU21_60088WSU21_60088genetic_marker
WSU21_60189WSU21_60189genetic_marker
WSU21_66524WSU21_66524genetic_marker
WSU21_67845WSU21_67845genetic_marker
WSU21_1_10107WSU21_1_10107genetic_marker
WSU21_1_10446WSU21_1_10446genetic_marker
WSU21_1_10520WSU21_1_10520genetic_marker
WSU21_1_11064WSU21_1_11064genetic_marker
WSU21_1_11194WSU21_1_11194genetic_marker
WSU21_1_11291WSU21_1_11291genetic_marker
WSU21_1_11419WSU21_1_11419genetic_marker
WSU21_1_11588WSU21_1_11588genetic_marker
WSU21_1_12562WSU21_1_12562genetic_marker
WSU21_1_13357WSU21_1_13357genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1664-8021
pISSN1664-8021
Publication Date2021
Journal AbbreviationFront Genet
DOI10.3389/fgene.2021.629267
Elocation10.3389/fgene.2021.629267
CopyrightCopyright © 2021 Williamson-Benavides, Sharpe, Nelson, Bodah, Porter and Dhingra.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland