Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)

Publication Overview
TitleConstruction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)
AuthorsSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S
TypeJournal Article
Journal NameMolecular Breeding
Volume35
Year2015
Page(s)185
CitationSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). 2015; 35:185.

Abstract

Field pea (Pisum sativum L.) is a grain legume crop that is cultivated for either human or livestock consumption. Development of varieties adapted to damaging abiotic and biotic stresses is a major objective for field pea breeding. Bacterial blight is a serious disease caused by the pathogenic agents Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. pisi. A recombinant inbred line (RIL) genetic mapping population was generated by crossing the susceptible genotype Kaspa to the resistant genotype PBA Oura. Previously described sets of single-nucleotide polymorphism and simple sequence repeat markers were assigned to a genetic linkage map of the Kaspa × PBA Oura population, which contained 358 markers spanning 1070 cM with an average marker density of 1 locus per 3.0 cM. Combination with multiple previously published maps (including that of Kaspa × Parafield) subsequently generated an integrated structure with much higher marker density of 1 locus per 0.85 cM. The Kaspa × PBA Oura and Kaspa × Parafield RILs were screened at the seedling stage for resistance to both pathovars. Totals of four and two QTLs for resistance to infection by P. syringae pv. syringae were detected in the Kaspa × Parafield and Kaspa × PBA Oura populations, respectively. A single common genomic region associated with P. syringae pv. pisi resistance was identified in both mapping populations. To integrate information on bacterial blight resistance from various QTL mapping studies, the relevant regions were extrapolated on to the integrated map through use of common flanking markers. The resources generated in this study will support map enhancement, identification of marker-trait associations, genomics-assisted breeding, map-based gene isolation and comparative genetics.
Features
This publication contains information about 3,014 features:
Feature NameUniquenameType
SNP_100000685SNP_100000685genetic_marker
SNP_100000686SNP_100000686genetic_marker
SNP_100000550SNP_100000550genetic_marker
SNP_100000238SNP_100000238genetic_marker
SNP_100000325SNP_100000325genetic_marker
SNP_100000364SNP_100000364genetic_marker
Pseudomonas syringae resistanceqPSR.KaspaxParafield.LGVIIQTL
Pseudomonas syringae resistanceqPSR.KaspaxParafield.LGIII.1QTL
Pseudomonas syringae resistanceqPSR.KaspaxParafield.LGIII.2QTL
Pseudomonas syringae resistanceqPSR.KaspaxParafield.LGIIQTL
Pseudomonas syringae resistanceqPSR.KaspaxParafield.LGIII.3QTL
Pseudomonas syringae resistanceQpsr.KaspaxPBA Oura.LGIII.1QTL
Pseudomonas syringae resistanceQpsr.KaspaxPBA Oura.LGVII.1QTL
Pseudomonas syringae resistanceQpsr.KaspaxPBA Oura.LGIII.2QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Bacterial_Blight_Resistance-Sudheesh-2015
Featuremaps
This publication contains information about 3 maps:
Map Name
Pea-Kaspa_x_PBA Oura-RIL
Pea-Integrated_Map-Sudheesh-2015
Pea-Composite_Map-Sudheesh-2015
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
KaspaKaspaaccession
PBA OuraPBA Ouraaccession
Kaspa_x_PBA OuraKaspa_x_PBA Ourapopulation
Properties
Additional details for this publication include:
Property NameValue
URLhttp://link.springer.com/article/10.1007/s11032-015-0376-4