Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)

Publication Overview
TitleConstruction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.)
AuthorsSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S
TypeJournal Article
Journal NameMolecular Breeding
Volume35
Year2015
Page(s)185
CitationSudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan NOI, Materne M, Forster JW, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). 2015; 35:185.

Abstract

Field pea (Pisum sativum L.) is a grain legume crop that is cultivated for either human or livestock consumption. Development of varieties adapted to damaging abiotic and biotic stresses is a major objective for field pea breeding. Bacterial blight is a serious disease caused by the pathogenic agents Pseudomonas syringae pv. syringae and Pseudomonas syringae pv. pisi. A recombinant inbred line (RIL) genetic mapping population was generated by crossing the susceptible genotype Kaspa to the resistant genotype PBA Oura. Previously described sets of single-nucleotide polymorphism and simple sequence repeat markers were assigned to a genetic linkage map of the Kaspa × PBA Oura population, which contained 358 markers spanning 1070 cM with an average marker density of 1 locus per 3.0 cM. Combination with multiple previously published maps (including that of Kaspa × Parafield) subsequently generated an integrated structure with much higher marker density of 1 locus per 0.85 cM. The Kaspa × PBA Oura and Kaspa × Parafield RILs were screened at the seedling stage for resistance to both pathovars. Totals of four and two QTLs for resistance to infection by P. syringae pv. syringae were detected in the Kaspa × Parafield and Kaspa × PBA Oura populations, respectively. A single common genomic region associated with P. syringae pv. pisi resistance was identified in both mapping populations. To integrate information on bacterial blight resistance from various QTL mapping studies, the relevant regions were extrapolated on to the integrated map through use of common flanking markers. The resources generated in this study will support map enhancement, identification of marker-trait associations, genomics-assisted breeding, map-based gene isolation and comparative genetics.
Features
This publication contains information about 3,014 features:
Feature NameUniquenameType
SNP_100000215_Ps001521SNP_100000215_Ps001521genetic_marker
SNP_100000659SNP_100000659genetic_marker
Ps000650Ps000650genetic_marker
SNP_100000356SNP_100000356genetic_marker
PsC19393p70PsC19393p70genetic_marker
PsC18864p85PsC18864p85genetic_marker
PsC13141p220PsC13141p220genetic_marker
Ps000431Ps000431genetic_marker
Ps001158Ps001158genetic_marker
Ps000489Ps000489genetic_marker
PsC16950p61_Ps000403PsC16950p61_Ps000403genetic_marker
Ps001380Ps001380genetic_marker
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Ps000618Ps000618genetic_marker
Ps000753Ps000753genetic_marker
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SNP_100000120SNP_100000120genetic_marker
PsC14749p226PsC14749p226genetic_marker
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Ps000536Ps000536genetic_marker
SNP_100000280SNP_100000280genetic_marker
Ps000127Ps000127genetic_marker
Ps000142Ps000142genetic_marker
Ps000164Ps000164genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Bacterial_Blight_Resistance-Sudheesh-2015
Featuremaps
This publication contains information about 3 maps:
Map Name
Pea-Kaspa_x_PBA Oura-RIL
Pea-Integrated_Map-Sudheesh-2015
Pea-Composite_Map-Sudheesh-2015
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
KaspaKaspaaccession
PBA OuraPBA Ouraaccession
Kaspa_x_PBA OuraKaspa_x_PBA Ourapopulation
Properties
Additional details for this publication include:
Property NameValue
URLhttp://link.springer.com/article/10.1007/s11032-015-0376-4