SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Publication Overview
TitleSNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil
AuthorsSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1604
CitationSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S. SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil. Frontiers in plant science. 2016; 7:1604.

Abstract

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible method for AB control. A detailed understanding of the genetic basis of AB resistance is hence highly desirable, in order to obtain insight into the number and influence of resistance genes. Genetic linkage maps based on single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers have been developed from three recombinant inbred line (RIL) populations. The IH × NF map contained 460 loci across 1461.6 cM, while the IH × DIG map contained 329 loci across 1302.5 cM and the third map, NF × DIG contained 330 loci across 1914.1 cM. Data from these maps were combined with a map from a previously published study through use of bridging markers to generate a consensus linkage map containing 689 loci distributed across seven linkage groups (LGs), with a cumulative length of 2429.61 cM at an average density of one marker per 3.5 cM. Trait dissection of AB resistance was performed for the RIL populations, identifying totals of two and three quantitative trait loci (QTLs) explaining 52 and 69% of phenotypic variation for resistance to infection in the IH × DIG and IH × NF populations, respectively. Presence of common markers in the vicinity of the AB_IH1- and AB_IH2.1/AB_IH2.2-containing regions on both maps supports the inference that a common genomic region is responsible for conferring resistance and is associated with the resistant parent, Indianhead. The third QTL was derived from Northfield. Evaluation of markers associated with AB resistance across a diverse lentil germplasm panel revealed that the identity of alleles associated with AB_IH1 predicted the phenotypic responses with high levels of accuracy (~86%), and therefore have the potential to be widely adopted in lentil breeding programs. The availability of RIL-based maps, a consensus map, and validated markers linked to AB resistance provide important resources for lentil improvement.

Features
This publication contains information about 686 features:
Feature NameUniquenameType
PBA_LC_0347PBA_LC_0347genetic_marker
SNP_20003185SNP_20003185genetic_marker
SNP_60000013SNP_60000013genetic_marker
SNP_60000014SNP_60000014genetic_marker
SNP_20003172SNP_20003172genetic_marker
PBA_LC_0379PBA_LC_0379genetic_marker
PBA_LC_0383PBA_LC_0383genetic_marker
SNP_20004666SNP_20004666genetic_marker
SNP_60000569SNP_60000569genetic_marker
SNP_20004461SNP_20004461genetic_marker
SNP_20000604SNP_20000604genetic_marker
SNP_20000864SNP_20000864genetic_marker
PBA_LC_0612PBA_LC_0612genetic_marker
SNP_20004665SNP_20004665genetic_marker
SNP_20003158SNP_20003158genetic_marker
SNP_20002199SNP_20002199genetic_marker
SNP_20001292SNP_20001292genetic_marker
SNP_20000615SNP_20000615genetic_marker
SNP_20003105SNP_20003105genetic_marker
SSR059SSR059genetic_marker
SNP_20001223SNP_20001223genetic_marker
SNP_20003160SNP_20003160genetic_marker
SNP_20003161SNP_20003161genetic_marker
SNP_20001205SNP_20001205genetic_marker
SNP_20004373SNP_20004373genetic_marker

Pages

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
IndianheadIndianheadaccession
NorthfieldNorthfieldaccession
DIGGERDIGGERaccession
Indianhead_x_NorthfieldIndianhead_x_Northfieldpopulation
Indianhead_x_DiggerIndianhead_x_Diggerpopulation
Northfield_x_DiggerNorthfield_x_Diggerpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountrySwitzerland