SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Publication Overview
TitleSNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil
AuthorsSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1604
CitationSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S. SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil. Frontiers in plant science. 2016; 7:1604.

Abstract

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible method for AB control. A detailed understanding of the genetic basis of AB resistance is hence highly desirable, in order to obtain insight into the number and influence of resistance genes. Genetic linkage maps based on single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers have been developed from three recombinant inbred line (RIL) populations. The IH × NF map contained 460 loci across 1461.6 cM, while the IH × DIG map contained 329 loci across 1302.5 cM and the third map, NF × DIG contained 330 loci across 1914.1 cM. Data from these maps were combined with a map from a previously published study through use of bridging markers to generate a consensus linkage map containing 689 loci distributed across seven linkage groups (LGs), with a cumulative length of 2429.61 cM at an average density of one marker per 3.5 cM. Trait dissection of AB resistance was performed for the RIL populations, identifying totals of two and three quantitative trait loci (QTLs) explaining 52 and 69% of phenotypic variation for resistance to infection in the IH × DIG and IH × NF populations, respectively. Presence of common markers in the vicinity of the AB_IH1- and AB_IH2.1/AB_IH2.2-containing regions on both maps supports the inference that a common genomic region is responsible for conferring resistance and is associated with the resistant parent, Indianhead. The third QTL was derived from Northfield. Evaluation of markers associated with AB resistance across a diverse lentil germplasm panel revealed that the identity of alleles associated with AB_IH1 predicted the phenotypic responses with high levels of accuracy (~86%), and therefore have the potential to be widely adopted in lentil breeding programs. The availability of RIL-based maps, a consensus map, and validated markers linked to AB resistance provide important resources for lentil improvement.

Features
This publication contains information about 686 features:
Feature NameUniquenameType
SNP_20003715SNP_20003715genetic_marker
SNP_20004843SNP_20004843genetic_marker
SNP_20003203SNP_20003203genetic_marker
SNP_20005072SNP_20005072genetic_marker
SNP_20001372SNP_20001372genetic_marker
SNP_20001829SNP_20001829genetic_marker
SNP_20001830SNP_20001830genetic_marker
SNP_20001370SNP_20001370genetic_marker
SNP_20001765SNP_20001765genetic_marker
SNP_20001754SNP_20001754genetic_marker
SNP_20003116SNP_20003116genetic_marker
SNP_20004199SNP_20004199genetic_marker
SNP_20004200SNP_20004200genetic_marker
SNP_20001054SNP_20001054genetic_marker
SNP_20003859SNP_20003859genetic_marker
SNP_20000378SNP_20000378genetic_marker
SNP_20004155SNP_20004155genetic_marker
SNP_20004154SNP_20004154genetic_marker
SNP_20003575SNP_20003575genetic_marker
SNP_60000200SNP_60000200genetic_marker
SNP_20003283SNP_20003283genetic_marker
SNP_20003281SNP_20003281genetic_marker
SNP_20001312SNP_20001312genetic_marker
SNP_20003360SNP_20003360genetic_marker
SNP_20000827SNP_20000827genetic_marker

Pages

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
IndianheadIndianheadaccession
NorthfieldNorthfieldaccession
DIGGERDIGGERaccession
Indianhead_x_NorthfieldIndianhead_x_Northfieldpopulation
Indianhead_x_DiggerIndianhead_x_Diggerpopulation
Northfield_x_DiggerNorthfield_x_Diggerpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountrySwitzerland