SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Publication Overview
TitleSNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil
AuthorsSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1604
CitationSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S. SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil. Frontiers in plant science. 2016; 7:1604.

Abstract

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible method for AB control. A detailed understanding of the genetic basis of AB resistance is hence highly desirable, in order to obtain insight into the number and influence of resistance genes. Genetic linkage maps based on single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers have been developed from three recombinant inbred line (RIL) populations. The IH × NF map contained 460 loci across 1461.6 cM, while the IH × DIG map contained 329 loci across 1302.5 cM and the third map, NF × DIG contained 330 loci across 1914.1 cM. Data from these maps were combined with a map from a previously published study through use of bridging markers to generate a consensus linkage map containing 689 loci distributed across seven linkage groups (LGs), with a cumulative length of 2429.61 cM at an average density of one marker per 3.5 cM. Trait dissection of AB resistance was performed for the RIL populations, identifying totals of two and three quantitative trait loci (QTLs) explaining 52 and 69% of phenotypic variation for resistance to infection in the IH × DIG and IH × NF populations, respectively. Presence of common markers in the vicinity of the AB_IH1- and AB_IH2.1/AB_IH2.2-containing regions on both maps supports the inference that a common genomic region is responsible for conferring resistance and is associated with the resistant parent, Indianhead. The third QTL was derived from Northfield. Evaluation of markers associated with AB resistance across a diverse lentil germplasm panel revealed that the identity of alleles associated with AB_IH1 predicted the phenotypic responses with high levels of accuracy (~86%), and therefore have the potential to be widely adopted in lentil breeding programs. The availability of RIL-based maps, a consensus map, and validated markers linked to AB resistance provide important resources for lentil improvement.

Features
This publication contains information about 686 features:
Feature NameUniquenameType
SNP_20003498SNP_20003498genetic_marker
SNP_20003502SNP_20003502genetic_marker
SNP_20000892SNP_20000892genetic_marker
SNP_20000825SNP_20000825genetic_marker
SNP_20001309SNP_20001309genetic_marker
SNP_20003282SNP_20003282genetic_marker
SNP_20001049SNP_20001049genetic_marker
SNP_20001052SNP_20001052genetic_marker
SNP_20000826SNP_20000826genetic_marker
SNP_20003499SNP_20003499genetic_marker
SNP_20003501SNP_20003501genetic_marker
SNP_20003504SNP_20003504genetic_marker
PBA_LC_0525PBA_LC_0525genetic_marker
SNP_20000822SNP_20000822genetic_marker
SNP_20005166SNP_20005166genetic_marker
SNP_20002288SNP_20002288genetic_marker
SNP_20003037SNP_20003037genetic_marker
SNP_20000956SNP_20000956genetic_marker
SNP_20000958SNP_20000958genetic_marker
SNP_20000955SNP_20000955genetic_marker
PBA_LC_0216PBA_LC_0216genetic_marker
SNP_20000957SNP_20000957genetic_marker
SNP_20000959SNP_20000959genetic_marker
SNP_20003060SNP_20003060genetic_marker
SNP_20004053SNP_20004053genetic_marker

Pages

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
IndianheadIndianheadaccession
NorthfieldNorthfieldaccession
DIGGERDIGGERaccession
Indianhead_x_NorthfieldIndianhead_x_Northfieldpopulation
Indianhead_x_DiggerIndianhead_x_Diggerpopulation
Northfield_x_DiggerNorthfield_x_Diggerpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountrySwitzerland