SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Publication Overview
TitleSNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil
AuthorsSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1604
CitationSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S. SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil. Frontiers in plant science. 2016; 7:1604.

Abstract

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible method for AB control. A detailed understanding of the genetic basis of AB resistance is hence highly desirable, in order to obtain insight into the number and influence of resistance genes. Genetic linkage maps based on single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers have been developed from three recombinant inbred line (RIL) populations. The IH × NF map contained 460 loci across 1461.6 cM, while the IH × DIG map contained 329 loci across 1302.5 cM and the third map, NF × DIG contained 330 loci across 1914.1 cM. Data from these maps were combined with a map from a previously published study through use of bridging markers to generate a consensus linkage map containing 689 loci distributed across seven linkage groups (LGs), with a cumulative length of 2429.61 cM at an average density of one marker per 3.5 cM. Trait dissection of AB resistance was performed for the RIL populations, identifying totals of two and three quantitative trait loci (QTLs) explaining 52 and 69% of phenotypic variation for resistance to infection in the IH × DIG and IH × NF populations, respectively. Presence of common markers in the vicinity of the AB_IH1- and AB_IH2.1/AB_IH2.2-containing regions on both maps supports the inference that a common genomic region is responsible for conferring resistance and is associated with the resistant parent, Indianhead. The third QTL was derived from Northfield. Evaluation of markers associated with AB resistance across a diverse lentil germplasm panel revealed that the identity of alleles associated with AB_IH1 predicted the phenotypic responses with high levels of accuracy (~86%), and therefore have the potential to be widely adopted in lentil breeding programs. The availability of RIL-based maps, a consensus map, and validated markers linked to AB resistance provide important resources for lentil improvement.

Features
This publication contains information about 686 features:
Feature NameUniquenameType
SNP_20002113SNP_20002113genetic_marker
SNP_20002981SNP_20002981genetic_marker
SNP_20002980SNP_20002980genetic_marker
SNP_20002116SNP_20002116genetic_marker
SNP_20005269SNP_20005269genetic_marker
SNP_20002350SNP_20002350genetic_marker
SNP_20003443SNP_20003443genetic_marker
SNP_20002969SNP_20002969genetic_marker
SNP_20003843SNP_20003843genetic_marker
SNP_20001693SNP_20001693genetic_marker
SNP_20001694SNP_20001694genetic_marker
PBA_LC_0629PBA_LC_0629genetic_marker
SNP_20005010SNP_20005010genetic_marker
SNP_20000625SNP_20000625genetic_marker
SNP_20005904SNP_20005904genetic_marker
SNP_20004043SNP_20004043genetic_marker
SNP_20003319SNP_20003319genetic_marker
SNP_20004042SNP_20004042genetic_marker
SNP_20000915SNP_20000915genetic_marker
SNP_20002328SNP_20002328genetic_marker
SNP_20002332SNP_20002332genetic_marker
SNP_20002197SNP_20002197genetic_marker
SNP_60000354SNP_60000354genetic_marker
SNP_20000149SNP_20000149genetic_marker
PBA_LC_0509PBA_LC_0509genetic_marker

Pages

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
IndianheadIndianheadaccession
NorthfieldNorthfieldaccession
DIGGERDIGGERaccession
Indianhead_x_NorthfieldIndianhead_x_Northfieldpopulation
Indianhead_x_DiggerIndianhead_x_Diggerpopulation
Northfield_x_DiggerNorthfield_x_Diggerpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountrySwitzerland