Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris)

Publication Overview
TitleAssociation mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris)
AuthorsSingh A, Sharma V, Dikshit HK, Aski M, Kumar H, Thirunavukkarasu N, Patil BS, Kumar S, Sarker A
TypeJournal Article
Journal NamePloS one
Volume12
Issue11
Year2017
Page(s)e0188296
CitationSingh A, Sharma V, Dikshit HK, Aski M, Kumar H, Thirunavukkarasu N, Patil BS, Kumar S, Sarker A. Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris). PloS one. 2017; 12(11):e0188296.

Abstract

Lentil is a major cool-season grain legume grown in South Asia, West Asia, and North Africa. Populations in developing countries of these regions have micronutrient deficiencies; therefore, breeding programs should focus more on improving the micronutrient content of food. In the present study, a set of 96 diverse germplasm lines were evaluated at three different locations in India to examine the variation in iron (Fe) and zinc (Zn) concentration and identify simple sequence repeat (SSR) markers that associate with the genetic variation. The genetic variation among genotypes of the association mapping (AM) panel was characterized using a genetic distance-based and a general model-based clustering method. The model-based analysis identified six subpopulations, which satisfactorily explained the genetic structure of the AM panel. AM analysis identified three SSRs (PBALC 13, PBALC 206, and GLLC 563) associated with grain Fe concentration explaining 9% to 11% of phenotypic variation and four SSRs (PBALC 353, SSR 317-1, PLC 62, and PBALC 217) were associated with grain Zn concentration explaining 14%, to 21% of phenotypic variation. These identified SSRs exhibited consistent performance across locations. These candidate SSRs can be used in marker-assisted genetic improvement for developing Fe and Zn fortified lentil varieties. Favorable alleles and promising genotypes identified in this study can be utilized for lentil biofortification.

Features
This publication contains information about 49 features:
Feature NameUniquenameType
PLC98PLC98genetic_marker
PLC100PLC100genetic_marker
PLC104PLC104genetic_marker
GLLC106GLLC106genetic_marker
GLLC108GLLC108genetic_marker
GLLC527GLLC527genetic_marker
GLLC538GLLC538genetic_marker
GLLC541GLLC541genetic_marker
GLLC562GLLC562genetic_marker
GLLC563GLLC563genetic_marker
GLLC598GLLC598genetic_marker
GLLC609GLLC609genetic_marker
GLLC614GLLC614genetic_marker
SSR132RNSSR132RNgenetic_marker
SSR230SSR230genetic_marker
SSR317-1SSR317-1genetic_marker
SSR66SSR66genetic_marker
SSR72SSR72genetic_marker
SSR302SSR302genetic_marker
SSR309-2SSR309-2genetic_marker
SSR48SSR48genetic_marker
SSR33SSR33genetic_marker
SSR233SSR233genetic_marker
PBA_LC_0013PBA_LC_0013genetic_marker

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Stocks
This publication contains information about 94 stocks:
Stock NameUniquenameType
ILL 2581ILL 2581accession
SKL 259SKL 259accession
EC 1EC 1accession
10-2-B-210-2-B-2accession
10-3-Y-2610-3-Y-26accession
Globe mutantGlobe mutantaccession
Fasciated mutantFasciated mutantaccession
HM 1HM 1accession
MC 6MC 6accession
K 75K 75accession
VL 103VL 103accession
FLIP 96–57FLIP 96–57accession
LL 147LL 147accession
LL 931LL 931accession
LC 74-1-5-1LC 74-1-5-1accession
LC 300–1LC 300–1accession
PL 4PL 4accession
LL 1231LL 1231accession
IPL 221IPL 221accession
VL 143VL 143accession
PL 406PL 406accession
PL 117PL 117accession
IPL 220IPL 220accession
L 4590L 4590accession
L 4591L 4591accession

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Properties
Additional details for this publication include:
Property NameValue
DOI10.1371/journal.pone.0188296
Elocation10.1371/journal.pone.0188296
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2017
Journal AbbreviationPLoS ONE
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States