Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris)

Publication Overview
TitleAssociation mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris)
AuthorsSingh A, Sharma V, Dikshit HK, Aski M, Kumar H, Thirunavukkarasu N, Patil BS, Kumar S, Sarker A
TypeJournal Article
Journal NamePloS one
Volume12
Issue11
Year2017
Page(s)e0188296
CitationSingh A, Sharma V, Dikshit HK, Aski M, Kumar H, Thirunavukkarasu N, Patil BS, Kumar S, Sarker A. Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris). PloS one. 2017; 12(11):e0188296.

Abstract

Lentil is a major cool-season grain legume grown in South Asia, West Asia, and North Africa. Populations in developing countries of these regions have micronutrient deficiencies; therefore, breeding programs should focus more on improving the micronutrient content of food. In the present study, a set of 96 diverse germplasm lines were evaluated at three different locations in India to examine the variation in iron (Fe) and zinc (Zn) concentration and identify simple sequence repeat (SSR) markers that associate with the genetic variation. The genetic variation among genotypes of the association mapping (AM) panel was characterized using a genetic distance-based and a general model-based clustering method. The model-based analysis identified six subpopulations, which satisfactorily explained the genetic structure of the AM panel. AM analysis identified three SSRs (PBALC 13, PBALC 206, and GLLC 563) associated with grain Fe concentration explaining 9% to 11% of phenotypic variation and four SSRs (PBALC 353, SSR 317-1, PLC 62, and PBALC 217) were associated with grain Zn concentration explaining 14%, to 21% of phenotypic variation. These identified SSRs exhibited consistent performance across locations. These candidate SSRs can be used in marker-assisted genetic improvement for developing Fe and Zn fortified lentil varieties. Favorable alleles and promising genotypes identified in this study can be utilized for lentil biofortification.

Stocks
This publication contains information about 94 stocks:
Stock NameUniquenameType
LL 1203LL 1203cultivar
RLG 147RLG 147cultivar
DL 11–4DL 11–4cultivar
KLS 113KLS 113cultivar
NDL 11–1NDL 11–1cultivar
PL 122PL 122cultivar
SKUAL 9SKUAL 9cultivar
L 4706L 4706cultivar
LL 1210LL 1210cultivar
KLB 345KLB 345cultivar
PL 024PL 024cultivar
PL 129PL 129cultivar
IPL 324IPL 324cultivar
IPL 406IPL 406cultivar
L 4707L 4707cultivar
LL 1204LL 1204cultivar
LH 84–8LH 84–8cultivar
RVL 48RVL 48cultivar
KLB 314KLB 314cultivar
IPL 325IPL 325cultivar
DPL 62DPL 62population
P 2102P 2102cultivar
P 2124P 2124cultivar
P 2125P 2125cultivar
P 2126P 2126cultivar

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Features
This publication contains information about 49 features:
Feature NameUniquenameType
GBSSR-VF-52GBSSR-VF-52genetic_marker
PLC5PLC5genetic_marker
PLC10PLC10genetic_marker
PLC16PLC16genetic_marker
PLC17PLC17genetic_marker
PLC21PLC21genetic_marker
PLC30PLC30genetic_marker
PLC35PLC35genetic_marker
PLC38PLC38genetic_marker
PLC39PLC39genetic_marker
PLC40PLC40genetic_marker
PLC42PLC42genetic_marker
PLC46PLC46genetic_marker
PLC51PLC51genetic_marker
PLC60PLC60genetic_marker
PLC62PLC62genetic_marker
PLC63PLC63genetic_marker
PLC74PLC74genetic_marker
PLC77PLC77genetic_marker
PLC80PLC80genetic_marker
PLC81PLC81genetic_marker
PLC83PLC83genetic_marker
PLC88PLC88genetic_marker
PLC95PLC95genetic_marker
PLC96PLC96genetic_marker

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Properties
Additional details for this publication include:
Property NameValue
DOI10.1371/journal.pone.0188296
Elocation10.1371/journal.pone.0188296
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2017
Journal AbbreviationPLoS ONE
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Cross References
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DatabaseAccession
PMID: PubMedPMID:29161321