A BAC/BIBAC-based physical map of chickpea, Cicer arietinum L

Publication Overview
TitleA BAC/BIBAC-based physical map of chickpea, Cicer arietinum L
AuthorsZhang X, Scheuring CF, Zhang M, Dong JJ, Zhang Y, Huang JJ, Lee MK, Abbo S, Sherman A, Shtienberg D, Chen W, Muehlbauer F, Zhang HB
TypeJournal Article
Journal NameBMC genomics
Volume11
Year2010
Page(s)501
CitationZhang X, Scheuring CF, Zhang M, Dong JJ, Zhang Y, Huang JJ, Lee MK, Abbo S, Sherman A, Shtienberg D, Chen W, Muehlbauer F, Zhang HB. A BAC/BIBAC-based physical map of chickpea, Cicer arietinum L. BMC genomics. 2010; 11:501.

Abstract

BACKGROUND
Chickpea (Cicer arietinum L.) is the third most important pulse crop worldwide. Despite its importance, relatively little is known about its genome. The availability of a genome-wide physical map allows rapid fine mapping of QTL, development of high-density genome maps, and sequencing of the entire genome. However, no such a physical map has been developed in chickpea.

RESULTS
We present a genome-wide, BAC/BIBAC-based physical map of chickpea developed by fingerprint analysis. Four chickpea BAC and BIBAC libraries, two of which were constructed in this study, were used. A total of 67,584 clones were fingerprinted, and 64,211 (~11.7 x) of the fingerprints validated and used in the physical map assembly. The physical map consists of 1,945 BAC/BIBAC contigs, with each containing an average of 28.3 clones and having an average physical length of 559 kb. The contigs collectively span approximately 1,088 Mb. By using the physical map, we identified the BAC/BIBAC contigs containing or closely linked to QTL4.1 for resistance to Didymella rabiei (RDR) and QTL8 for days to first flower (DTF), thus further verifying the physical map and confirming its utility in fine mapping and cloning of QTL.

CONCLUSION
The physical map represents the first genome-wide, BAC/BIBAC-based physical map of chickpea. This map, along with other genomic resources previously developed in the species and the genome sequences of related species (soybean, Medicago and Lotus), will provide a foundation necessary for many areas of advanced genomics research in chickpea and other legume species. The inclusion of transformation-ready BIBACs in the map greatly facilitates its utility in functional analysis of the legume genomes.

Features
This publication contains information about 5 features:
Feature NameUniquenameType
Didymella rabiei resistanceqRDR.Hadas.LG4.QTL4.1QTL
Didymella rabiei resistanceqRDR.Hadas.LG4.QTL4.2QTL
Didymella rabiei resistanceqRDR.Hadas.LG4.QTL8QTL
Days to first flowerqDFFL.Hadas.LG8.GilatQTL
Days to first flowerqDFFL.Hadas.LG8.MassuotQTL
Projects
This publication contains information about 1 projects:
Project NameDescription
Chickpea-didymella_rabei_resistance-days_to_first_flower-Zhang-2010
Featuremaps
This publication contains information about 1 maps:
Map Name
chickpea-Hadas-2010
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication TypeJournal Article
LanguageEnglish
Journal CountryEngland
Publication ModelElectronic
ISSN1471-2164
eISSN1471-2164
Publication Date2010
Journal AbbreviationBMC Genomics
DOI10.1186/1471-2164-11-501
Elocation10.1186/1471-2164-11-501
Publication TypeResearch Support, Non-U.S. Gov't