Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]

Publication Overview
TitleGenetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]
AuthorsGnanesh BN, Bohra A, Sharma M, Byregowda M, Pande S, Wesley V, Saxena RK, Saxena KB, Kishor PBK, Varshney RK
TypeJournal Article
Journal NameField Crops Research
Volume123
Year2011
Page(s)53-61
CitationGnanesh BN, Bohra A, Sharma M, Byregowda M, Pande S, Wesley V, Saxena RK, Saxena KB, Kishor PBK, Varshney RK. Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Field Crops Research. 2011; 123:53-61.

Abstract

Sterility mosaic disease (SMD), considered as the “green plague of pigeonpea” and caused by pigeonpea sterility mosaic virus (PPSMV) is one of the major biotic factors, which leads to heavy yield losses and hence poses a big challenge for pigeonpea production in the Indian subcontinent. Variability in the sterility mosaic pathogen revealed the occurrence of five different isolates in India. Among them, three distinct SMD isolates have been characterized, viz., Patancheru, Bangalore and Coimbatore. Molecular tools offer a viable option to tackle these biotic stresses via identification of the genomic regions associated with the trait such as SMD resistance. With an aim of identifying the gene(s)/QTLs linked with SMD resistance, two F2 populations, i.e. ICP 8863×ICPL 20097 (segregating for Patancheru SMD isolate) and TTB 7×ICP 7035 (segregating for both Patancheru and Bangalore SMD isolates) were developed and F2:3 families were phenotyped for resistance to respective isolate(s) of SMD. After screening over 3000 SSR markers on parental genotypes of each mapping population, intra-specific genetic maps comprising of 11 linkage groups and 120 and 78 SSR loci were developed for ICP 8863×ICPL 20097 and TTB 7×ICP 7035 populations, respectively. Composite interval mapping (CIM) based QTL analysis by using genetic mapping and phenotyping data provided four QTLs for Patancheru SMD isolate and two QTLs for Bangalore SMD isolate. Identification of different QTLs for resistance to Patancheru and Bangalore SMD isolates is an indication of involvement of different genes conferring the resistance to these two SMD isolates. One QTL namely qSMD4 identified within an interval of 2.8cM on LG 7 explaining 24.72% of phenotypic variance, once it is validated in other genetic background, seems to be a promising QTL for use in marker assisted selection. In summary, this is the first study on development of intra-specific genetic maps and identification of QTLs for SMD resistance in pigeonpea.
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
ICP_8863ICP_8863breeding_research_material
TTB_7TTB_7breeding_research_material
ICPL_20097ICPL_20097breeding_research_material
ICP_7035ICP_7035breeding_research_material
ICP_8863/ICPL_20097-F2ICP_8863/ICPL_20097-F2population
TTB_7/ICP_7035-F2TTB_7/ICP_7035-F2population
Features
This publication contains information about 8 features:
Feature NameUniquenameType
Sterility mosaic disease resistanceqSMD.TTB_7/ICP_7035.LG01QTL
Sterility mosaic disease resistanceqSMD.TTB_7/ICP_7035.LG07QTL
Sterility mosaic disease resistanceqSMD.TTB_7/ICP_7035.LG03QTL
Sterility mosaic disease resistanceqSMD.TTB_7/ICP_7035.LG02QTL
Sterility mosaic disease resistanceqSMD.ICP_8863/ICPL_20097.LG09.2QTL
Sterility mosaic disease resistanceqSMD.ICP_8863/ICPL_20097.LG09QTL
ICPeM0075ICPeM0075genetic_marker
Ccac0035Ccac0035genetic_marker
Projects
This publication contains information about 1 projects:
Project NameDescription
Pigeon_pea-Sterility_mosaic_disease-Gnanesh-2011
Featuremaps
This publication contains information about 2 maps:
Map Name
Pigeon_pea-ICP_8863/ICPL_20097-F2
Pigeon_pea-TTB_7/ICP_7035-F2