A second VrPGIP1 allele is associated with bruchid resistance (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) accession ACC41.

Publication Overview
TitleA second VrPGIP1 allele is associated with bruchid resistance (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) accession ACC41.
AuthorsKaewwongwal A, Liu C, Somta P, Chen J, Tian J, Yuan X, Chen X
TypeJournal Article
Journal NameMolecular genetics and genomics : MGG
Year2019
CitationKaewwongwal A, Liu C, Somta P, Chen J, Tian J, Yuan X, Chen X. A second VrPGIP1 allele is associated with bruchid resistance (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) accession ACC41.. Molecular genetics and genomics : MGG. 2019 Nov 08.

Abstract

Two bruchid species, azuki bean weevil (Callosobruchus chinensis L.) and cowpea weevil (Callosobruchus maculatus F.), are the most important insect pests of mungbean [Vigna radiata (L.) Wilczek] after harvest. Improving bruchid resistance is a major goal for mungbean breeders. Bruchid resistance in mungbean is controlled by a single major locus, Br. The tightly linked VrPGIP1 and VrPGIP2, which encode polygalacturonase-inhibiting proteins (PGIPs), are the candidate genes at the Br locus associated with bruchid resistance. One VrPGIP1 resistance allele and two VrPGIP2 resistance alleles have been identified. In this study, we fine-mapped the bruchid-resistance genes in wild mungbean (V. radiata var. sublobata) accession ACC41 using the F2 population (574 individuals) derived from the 'Kamphaeng Saen 2' (susceptible) × ACC41 (resistant) cross. A QTL analysis indicated that the resistance to the azuki bean weevil and cowpea weevil in ACC41 is controlled by a major QTL (qBr5.1) and a minor QTL (qBr5.2), which are only 0.3 cM apart. qBr5.1 and qBr5.2 accounted for about 82% and 2% of the resistance variation in the F2 population, respectively. qBr5.1 was mapped to a 237.35-kb region on mungbean chromosome 5 containing eight annotated genes, including VrPGIP1 and VrPGIP2. An examination of the ACC41 VrPGIP1 and VrPGIP2 sequences revealed a new allele for VrPGIP1 (i.e., VrPGIP1-2). Compared with the wild-type sequence, VrPGIP1-2 has five SNPs, of which four cause amino acid changes (residues 125, 129, 188, and 336). A protein sequence analysis indicated that residues 125 and 129 in VrPGIP1-2 are in a β-sheet B1 region, whereas residues 188 and 336 are in a C10-helix region and at the end of the C-terminal region, respectively. Because the β-sheet B1 region is important for interactions with polygalacturonase (PG), residues 125 and 129 in VrPGIP1-2 likely contribute to bruchid resistance by inhibiting PG. Our results imply that VrPGIP1-2 is associated with the bruchid resistance of wild mungbean accession ACC41. This new resistance allele may be useful for breeding mungbean varieties exhibiting durable bruchid resistance.

Features
This publication contains information about 104 features:
Feature NameUniquenameType
VrBr001VrBr001genetic_marker
VrBr002VrBr002genetic_marker
VrBr003VrBr003genetic_marker
VrBr004VrBr004genetic_marker
VrBr005VrBr005genetic_marker
VrBr006VrBr006genetic_marker
VrBr007VrBr007genetic_marker
VrBr008VrBr008genetic_marker
VrBr009VrBr009genetic_marker
VrBr010VrBr010genetic_marker
VrBr011VrBr011genetic_marker
VrBr012VrBr012genetic_marker
VrBr013VrBr013genetic_marker
VrBr014VrBr014genetic_marker
VrBr015VrBr015genetic_marker
VrBr016VrBr016genetic_marker
VrBr017VrBr017genetic_marker
VrBr018VrBr018genetic_marker
VrBr019VrBr019genetic_marker
VrBr020VrBr020genetic_marker
VrBr021VrBr021genetic_marker
VrBr022VrBr022genetic_marker
VrBr023VrBr023genetic_marker
VrBr024VrBr024genetic_marker
VrBr025VrBr025genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Vigna-Bruchid_resistance-Kaewwongwal-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Vigna-KPS2/ACC41-F2
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
ACC41ACC41accession
KPS2/ACC41-F2KPS2/ACC41-F2population
Kamphaeng Saen 2Kamphaeng Saen 2accession
Properties
Additional details for this publication include:
Property NameValue
Publication Date2019 Nov 08
Journal AbbreviationMol. Genet. Genomics
DOI10.1007/s00438-019-01619-y
Elocation10.1007/s00438-019-01619-y
Publication ModelPrint-Electronic
ISSN1617-4623
eISSN1617-4623
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany