Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis

Publication Overview
TitleRetrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis
AuthorsFlavell AJ, Knox MR, Pearce SR, Ellis TH
TypeJournal Article
Journal NameThe Plant journal : for cell and molecular biology
Volume16
Issue5
Year1998
Page(s)643-50
CitationFlavell AJ, Knox MR, Pearce SR, Ellis TH. Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis. The Plant journal : for cell and molecular biology. 1998 Dec; 16(5):643-50.

Abstract

Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co-dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN0960-7412
pISSN0960-7412
Publication Date1998 Dec
Journal AbbreviationPlant J.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryENGLAND
Publication TypeResearch Support, Non-U.S. Gov't
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:10036780
AGL: USDA National Agricultural LibraryAGL:3349035