Linkage map development by GBS, SSR, and SRAP techniques and yield-related QTLs in pea

Publication Overview
TitleLinkage map development by GBS, SSR, and SRAP techniques and yield-related QTLs in pea
AuthorsGuindon MF, Martin E, Cravero V, Gali KK, Warkentin TD, Cointry E
TypeJournal Article
Journal NameMolecular breeding
Volume39
Issue4
Year2019
Page(s)54
CitationGuindon MF, Martin E, Cravero V, Gali KK, Warkentin TD, Cointry E. Linkage map development by GBS, SSR, and SRAP techniques and yield-related QTLs in pea. Molecular breeding. 2019; 39(4):54.

Abstract

The knowledge of the genetic basis of yield-related traits provides useful information for pea (Pisum sativum L.) breeding. The aim of this work was to develop a genetic linkage map of pea using SRAP (sequence-related amplified polymorphism), SSR (simple sequence repeat), and SNP (single-nucleotide polymorphism) markers and to identify QTLs (quantitative trait loci) controlling yield-related traits. An F2 mapping population and their F2:3 families were derived from an initial cross between cvs. Explorer and DDR14. The F2 individuals were evaluated with SRAP, SSR, and GBS techniques, which proved to be efficient, generating a set of 872 polymorphic markers for linkage mapping. The resulting map consisted of 128 genetic markers distributed in 9 linkage groups (LGs), covering 655.5 cM. The length of the LGs ranged from 49.1 to 114.8 cM, with 8 to 26 markers. QTL detection was performed using the composite interval mapping (CIM) method. A total of 45 QTLs were detected through the generations and environments evaluated. All of them were major QTLs that explain more than 10% of the phenotypic variation. The comparison among QTLs detected in the F2 population and F2:3 families in the two environments allowed assessing the consistency of two QTLs for pod width. On the other hand, the analysis of previous works allowed the identification of a common region in LG 3 associated with seed weight in different crosses and environments.
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
DDR14DDR14cultivar
ExplorerExplorercultivar
DDR14/Explorer-F2DDR14/Explorer-F2population
DDR14/Explorer-F2:3DDR14/Explorer-F2:3population
Features
This publication contains information about 105 features:
Feature NameUniquenameType
me10em10me10em10genetic_marker
me10em6me10em6genetic_marker
me10em7me10em7genetic_marker
me10em8me10em8genetic_marker
me10em9me10em9genetic_marker
me1em2me1em2genetic_marker
me1em3me1em3genetic_marker
me1em4me1em4genetic_marker
me1em5me1em5genetic_marker
me2em1me2em1genetic_marker
me2em2me2em2genetic_marker
me2em4me2em4genetic_marker
me2em5me2em5genetic_marker
me3em2me3em2genetic_marker
me3em3me3em3genetic_marker
me3em4me3em4genetic_marker
me3em5me3em5genetic_marker
me4em2me4em2genetic_marker
me4em3me4em3genetic_marker
me4em4me4em4genetic_marker
me4em5me4em5genetic_marker
me5em2me5em2genetic_marker
me5em4me5em4genetic_marker
me5em5me5em5genetic_marker
me6em6me6em6genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Yield_traits-Guindon-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Pea-DDR14/Explorer-F2
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date2019
Publication TypeJournal Article
URLhttps://dx.doi.org/10.1007/s11032-019-0949-8
KeywordsPisum sativum, chromosome mapping, crossing, genetic markers, linkage groups, microsatellite repeats, peas, phenotypic variation, quantitative trait loci, seed weight, single nucleotide polymorphism
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:6541092