Candidate gene mapping reveals VrMLO12 (MLO Clade II) is associated with powdery mildew resistance in mungbean (Vigna radiata [L.] Wilczek).

Publication Overview
TitleCandidate gene mapping reveals VrMLO12 (MLO Clade II) is associated with powdery mildew resistance in mungbean (Vigna radiata [L.] Wilczek).
AuthorsYundaeng C, Somta P, Chen J, Yuan X, Chankaew S, Srinives P, Chen X
TypeJournal Article
Journal NamePlant science : an international journal of experimental plant biology
Volume298
Year2020
Page(s)110594
CitationYundaeng C, Somta P, Chen J, Yuan X, Chankaew S, Srinives P, Chen X. Candidate gene mapping reveals VrMLO12 (MLO Clade II) is associated with powdery mildew resistance in mungbean (Vigna radiata [L.] Wilczek).. Plant science : an international journal of experimental plant biology. 2020 Sep; 298:110594.

Abstract

Loss/reduction of function of Mildew Locus O (MLO) genes clade V and MLO clade IV has been shown to be responsible for powdery mildew (PM) resistance in several plant species. Mungbean (Vigna radiata) genome possesses 18 MLO genes, VrMLO1 - VrMLO18. A previous study using mungbean F2 and BC1F1 populations derived from a cross between "CN60″ (susceptible) and "RUM5″ (resistance) demonstrated that QTL qPMRUM5-3 is a major QTL for PM resistance caused by Erysiphe polygoni and is the same with major QTL qPMV4718-3 that confers PM resistance in "V4718″ (resistance). In this study, bioinformatics analysis revealed VrMLO12 locates in the qPMRUM5-3 region. Fine mapping in the F2 and BC1F1 populations using newly developed DNA markers including gene-specific markers demonstrated association between VrMLO12 and the PM resistance. Sequence analyses of VrMLO12 revealed that compared to susceptible mungbeans, RUM5 and V4718 possess SNPs in exon 10 and exon 13. The SNPs caused amino acid changes of VrMLO12, A387S and A476 G, respectively. The change occurred in transmembrane 6 domain and calmodulin binding domain (CaMBD) of the VrMLO12 protein, respectively. qRT-PCR showed that transcript expression level of VrMLO12 in RUM5 challenged with and without by E. polygoni was significantly higher than that in CN60. Phylogenetic analysis revealed that in contrast to previous findings that MLO proteins associated with PM resistance belong to MLO clade V and MLO clade IV, VrMLO12 belongs to MLO clade II. The result suggested that VrMLO12 may function differently from the other MLOs that associated with PM susceptibility. Our findings provide insight into the PM resistance in mungbean and tools for mungbean breeding.

Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
RUM5RUM5breeding_research_material
Chai Nat 60Chai Nat 60cultivar
CN60/RUM5-F2CN60/RUM5-F2population
CN60/RUM5-BC1F1CN60/RUM5-BC1F1population
Features
This publication contains information about 62 features:
Feature NameUniquenameType
VrMLO12_Indel1VrMLO12_Indel1genetic_marker
VrMLO12_Indel2VrMLO12_Indel2genetic_marker
VrMLO12_Indel3VrMLO12_Indel3genetic_marker
JAAS_Vr_SNAP1JAAS_Vr_SNAP1genetic_marker
JAAS_Vr_SNAP2JAAS_Vr_SNAP2genetic_marker
Vr9gSSR01Vr9gSSR01genetic_marker
Vr9gSSR02Vr9gSSR02genetic_marker
Vr9gSSR03Vr9gSSR03genetic_marker
Vr9gSSR04Vr9gSSR04genetic_marker
Vr9gSSR05Vr9gSSR05genetic_marker
Vr9gSSR06Vr9gSSR06genetic_marker
Vr9gSSR07Vr9gSSR07genetic_marker
Vr9gSSR08Vr9gSSR08genetic_marker
Vr9gSSR09Vr9gSSR09genetic_marker
Vr9gSSR10Vr9gSSR10genetic_marker
Vr9gSSR11Vr9gSSR11genetic_marker
Vr9gSSR12Vr9gSSR12genetic_marker
Vr9gSSR13Vr9gSSR13genetic_marker
Vr9gSSR14Vr9gSSR14genetic_marker
Vr9gSSR15Vr9gSSR15genetic_marker
Vr9gSSR16Vr9gSSR16genetic_marker
Vr9gSSR17Vr9gSSR17genetic_marker
Vr9gSSR18Vr9gSSR18genetic_marker
Vr9gSSR19Vr9gSSR19genetic_marker
Vr9gSSR20Vr9gSSR20genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Vigna-Powdery_mildew_resistance-Yundaeng-2020
Featuremaps
This publication contains information about 2 maps:
Map Name
Vigna-CN60/RUM5-F2
Vigna-CN60/RUM5-BC1F1
Properties
Additional details for this publication include:
Property NameValue
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryIreland
Publication ModelPrint-Electronic
ISSN1873-2259
eISSN1873-2259
Publication Date2020 Sep
Journal AbbreviationPlant Sci.
PIIS0168-9452(20)30200-4
Elocation10.1016/j.plantsci.2020.110594
DOI10.1016/j.plantsci.2020.110594
CopyrightCopyright © 2020 Elsevier B.V. All rights reserved.
Cross References
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DatabaseAccession
PMID: PubMedPMID:32771151