Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.

Publication Overview
TitleMapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.
AuthorsWu L, Fredua-Agyeman R, Hwang SF, Chang KF, Conner RL, McLaren DL, Strelkov SE
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2021
CitationWu L, Fredua-Agyeman R, Hwang SF, Chang KF, Conner RL, McLaren DL, Strelkov SE. Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2021 Jun 15.

Abstract

KEY MESSAGE
A stable and major QTL, which mapped to an approximately 20.0 cM region on pea chromosome 4, was identified as the most consistent region conferring partial resistance to Aphanomyces euteiches. Aphanomyces root rot (ARR), caused by Aphanomyces euteiches Drechs., is a destructive soilborne disease of field pea (Pisum Sativum L.). No completely resistant pea germplasm is available, and current ARR management strategies rely on partial resistance and fungicidal seed treatments. In this study, an F8 recombinant inbred line population of 135 individuals from the cross 'Reward' (susceptible) × '00-2067' (tolerant) was evaluated for reaction to ARR under greenhouse conditions with the A. euteiches isolate Ae-MDCR1 and over 2 years in a field nursery in Morden, Manitoba. Root rot severity, foliar weight, plant vigor and height were used as estimates of tolerance to ARR. Genotyping was conducted with a 13.2 K single-nucleotide polymorphism (SNP) array and 222 simple sequence repeat (SSR) markers. Statistical analyses of the phenotypic data indicated significant (P < 0.001) genotypic effects and significant G × E interactions (P < 0.05) in all experiments. After filtering, 3050 (23.1%) of the SNP and 30 (13.5%) of the SSR markers were retained for linkage analysis, which distributed 2999 (2978 SNP + 21 SSR) of the markers onto nine linkage groups representing the seven chromosomes of pea. Mapping of quantitative trait loci (QTL) identified 8 major-effect (R2 > 20%), 13 moderate-effect (10% < R2 < 20%) effect and 6 minor-effect (R2 < 10%) QTL. A genomic region on chromosome 4, delimited by the SNP markers PsCam037549_22628_1642 and PsCam026054_14999_2864, was identified as the most consistent region responsible for partial resistance to A. euteiches isolate Ae-MDCR1. Other genomic regions important for resistance were of the order chromosome 5, 6 and 7.

Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
00-206700-2067cultivar
RewardRewardcultivar
00-2067/Reward-F8-RIL00-2067/Reward-F8-RILcultivar
Features
This publication contains information about 56 features:
Feature NameUniquenameType
AA372AA372genetic_marker
Aphanomyces root rot resistanceqARRR.002067/Reward.LG03.15QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG03.16QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG03.GHQTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG04QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG04.GHQTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG04.GH2QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05.16QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05.GHQTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05.GH1QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05.GH2QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG05.GH3QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG09QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG09.16QTL
Aphanomyces root rot resistanceqARRR.002067/Reward.LG09.16.2QTL
Leaf dry weightqLFDW.002067/Reward.LG03QTL
Leaf dry weightqLFDW.002067/Reward.LG03.16QTL
Leaf dry weightqLFDW.002067/Reward.LG03.16.2QTL
Leaf dry weightqLFDW.002067/Reward.LG05QTL
Leaf dry weightqLFDW.002067/Reward.LG05.16QTL
Leaf dry weightqLFDW.002067/Reward.LG05.GH1QTL
Leaf dry weightqLFDW.002067/Reward.LG05.GH2QTL
Leaf dry weightqLFDW.002067/Reward.LG08.16QTL
Leaf dry weightqLFDW.002067/Reward.LG08.16.2QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Aphanomyces_root_rot-Wu-2021
Featuremaps
This publication contains information about 1 maps:
Map Name
Pea-002067/Reward-F8-RIL
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2021 Jun 15
Journal AbbreviationTheor Appl Genet
DOI10.1007/s00122-021-03871-6
Elocation10.1007/s00122-021-03871-6
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:34129066