Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba)

Publication Overview
TitleDevelopment and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba)
AuthorsZanotto S, Vandenberg A, Khazaei H
TypeJournal Article
Journal NamePlant Breeding
Volume139
Year2020
Page(s)375-380
CitationZanotto S, Vandenberg A, Khazaei H. Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba). Plant Breeding. 2020; 137:375-380.

Abstract

Faba bean has the potential to become a key food and feed protein crop in many areas of the world. The presence of tannins in its seed coat has limited the deployment of this crop as feed and food. The expression of either of the two recessive genes, zt1 and zt2, causes a great reduction of tannins from the seed coat and results in a white flower phenotype. Molecular markers linked to these loci are fundamental tools for speeding up the breeding of low‐tannin varieties. The main aim of this study was to develop and validate a robust molecular marker linked to the zt2 locus. We used 176 recombinant inbred lines of the Disco/2 × ILB 938/2 cross at F6 and genotyped those using 257 SNP (single nucleotide polymorphism) markers. An SNP marker associated with zt2 locus was found on faba bean chromosome 3 and was used to develop a high‐throughput low‐cost KASP (kompetitive allele‐specific PCR) marker. The KASP marker can successfully discriminate low‐tannin faba beans carrying zt2 from those carrying zt1 and wild‐type alleles. KEYWORDS broad bean, candidate gene, flower colour, molecular breeding, SNP marker, tannins.
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
Disco/2Disco/2cultivar
Disco/ILB938-F6-RILDisco/ILB938-F6-RILpopulation
Features
This publication contains information about 1 features:
Feature NameUniquenameType
INRA_KASP_zt2INRA_KASP_zt2genetic_marker
Featuremaps
This publication contains information about 1 maps:
Map Name
Faba-Disco/ILB938-F6-RIL
Properties
Additional details for this publication include:
Property NameValue
URLhttps://onlinelibrary.wiley.com/doi/full/10.1111/pbr.12772