Identification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.).

Publication Overview
TitleIdentification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.).
AuthorsYang X, Liu C, Li Y, Yan Z, Liu D, Feng G
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2021
CitationYang X, Liu C, Li Y, Yan Z, Liu D, Feng G. Identification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.).. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2021 Aug 02.

Abstract

KEY MESSAGE
Using bulked segregant analysis combined with next-generation sequencing, we delimited the pv-ye gene responsible for the golden pod trait of snap bean cultivar A18-1. Sequence analysis identified Phvul.002G006200 as the candidate gene. The pod is the main edible part of snap beans (Phaseolus vulgaris L.). The commercial use of the pods is mainly affected by their color. Consumers seem to prefer golden pods. The aim of the present study was to identify the gene responsible for the golden pod trait in the snap bean. 'A18-1' (a golden bean cultivar) and 'Renaya' (a green bean cultivar) were chosen as the experimental materials. Genetic analysis indicated that a single recessive gene, pv-ye, controls the golden pod trait. A candidate region of 4.24 Mb was mapped to chromosome Pv 02 using bulked-segregant analysis coupled with whole-genome sequencing. In this region, linkage analysis in an F2 population localized the pv-ye gene to an interval of 182.9 kb between the simple sequence repeat markers SSR77 and SSR93. This region comprised 16 genes (12 annotated genes from the P. vulgaris database and 4 functionally unknown genes). Combined with transcriptome sequencing results, we identified Phvul.002G006200 as the potential candidate gene for pv-ye. Sequencing of Phvul.002G006200 identified a single-nucleotide polymorphism (SNP) in pv-ye. A pair of primers covering the SNP were designed, and the fragment was sequenced to screen 1086 F2 plants with the 'A18-1' phenotype. Our findings showed that among the 1086 mapped individuals, the SNP cosegregated with the 'A18-1' phenotype. The findings presented here could form the basis to reveal the molecular mechanism of the golden pod trait in the snap bean.

Features
This publication contains information about 7 features:
Feature NameUniquenameType
HLJU21_SSR03HLJU21_SSR03genetic_marker
HLJU21_SSR12HLJU21_SSR12genetic_marker
HLJU21_SSR23HLJU21_SSR23genetic_marker
HLJU21_SSR31HLJU21_SSR31genetic_marker
HLJU21_SSR54HLJU21_SSR54genetic_marker
HLJU21_SSR77HLJU21_SSR77genetic_marker
HLJU21_SSR93HLJU21_SSR93genetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2021 Aug 02
Journal AbbreviationTheor Appl Genet
DOI10.1007/s00122-021-03928-6
Elocation10.1007/s00122-021-03928-6
Copyright© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
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DatabaseAccession
PMID: PubMedPMID:34338807