In silico development and validation of EST derived new SSR markers for drought tolerance in Cicer arietinum L.

Publication Overview
TitleIn silico development and validation of EST derived new SSR markers for drought tolerance in Cicer arietinum L.
AuthorsBhardway J, Kumari N, Ford R, Yadav R, Choi I, Kumar R
TypeJournal Article
Journal NameIndian Journal of Genetics
Volume74
Issue2
Year2014
Page(s)254-256
CitationBhardway J, Kumari N, Ford R, Yadav R, Choi I, Kumar R. In silico development and validation of EST derived new SSR markers for drought tolerance in Cicer arietinum L. Indian J Genet. 2014; 74(2):254-256.

Abstract

Microsatellites have become a marker of choice in plant genetics and breeding research due to their unilocus, multiallelic and highly reproducible nature. In present study EST sequences isolated from chickpea root under water stress were assessed for the frequency, type and distribution of SSR motifs. Out of the 6400 ESTs (accession no. CDO 38847 to GR 394575), SSR sequences were identified in 348 singleton contigs and from these, 50 flanking primer pairs were designed. Out of these, 41 amplified a marker of the expected size range across nineteen diverse chickpea accessions. 11 of the primer pairs were size polymorphic with an average PIC value of 0.59 across the nineteen genotypes assessed.
Features
This publication contains information about 11 features:
Feature NameUniquenameType
SVP11SVP11genetic_marker
SVP162SVP162genetic_marker
SVP180SVP180genetic_marker
SVP181SVP181genetic_marker
SVP215SVP215genetic_marker
SVP219SVP219genetic_marker
SVP221SVP221genetic_marker
SVP309SVP309genetic_marker
SVP31SVP31genetic_marker
SVP311SVP311genetic_marker
SVP64SVP64genetic_marker
Properties
Additional details for this publication include:
Property NameValue
URLhttps://www.isgpb.org/documents/archive/art-16-254-256.pdf