Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance.

Publication Overview
TitleGenome-wide association study reveals white lupin candidate gene involved in anthracnose resistance.
AuthorsAlkemade JA, Nazzicari N, Messmer MM, Annicchiarico P, Ferrari B, Voegele RT, Finckh MR, Arncken C, Hohmann P
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2022
CitationAlkemade JA, Nazzicari N, Messmer MM, Annicchiarico P, Ferrari B, Voegele RT, Finckh MR, Arncken C, Hohmann P. Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance.. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2022 Jan 05.

Abstract

KEY MESSAGE
GWAS identifies candidate gene controlling resistance to anthracnose disease in white lupin. White lupin (Lupinus albus L.) is a promising grain legume to meet the growing demand for plant-based protein. Its cultivation, however, is severely threatened by anthracnose disease caused by the fungal pathogen Colletotrichum lupini. To dissect the genetic architecture for anthracnose resistance, genotyping by sequencing was performed on white lupin accessions collected from the center of domestication and traditional cultivation regions. GBS resulted in 4611 high-quality single-nucleotide polymorphisms (SNPs) for 181 accessions, which were combined with resistance data observed under controlled conditions to perform a genome-wide association study (GWAS). Obtained disease phenotypes were shown to highly correlate with overall three-year disease assessments under Swiss field conditions (r > 0.8). GWAS results identified two significant SNPs associated with anthracnose resistance on gene Lalb_Chr05_g0216161 encoding a RING zinc-finger E3 ubiquitin ligase which is potentially involved in plant immunity. Population analysis showed a remarkably fast linkage disequilibrium decay, weak population structure and grouping of commercial varieties with landraces, corresponding to the slow domestication history and scarcity of modern breeding efforts in white lupin. Together with 15 highly resistant accessions identified in the resistance assay, our findings show promise for further crop improvement. This study provides the basis for marker-assisted selection, genomic prediction and studies aimed at understanding anthracnose resistance mechanisms in white lupin and contributes to improving breeding programs worldwide.

Features
This publication contains information about 10 features:
Feature NameUniquenameType
Lalb_ Chr05_2957601Lalb_ Chr05_2957601genetic_marker
Lalb_ Chr05_2957940Lalb_ Chr05_2957940genetic_marker
Lalb_ Chr05_3706534Lalb_ Chr05_3706534genetic_marker
Anthracnose disease score - stemGWAS0000046GWAS
Anthracnose disease score - stemGWAS0000047GWAS
Anthracnose relative lesion sizeGWAS0000048GWAS
Anthracnose relative lesion sizeGWAS0000049GWAS
Relative shoot fresh weightGWAS0000050GWAS
Relative shoot fresh weightGWAS0000051GWAS
Anthracnose relative lesion sizeGWAS0000052GWAS
Projects
This publication contains information about 1 projects:
Project NameDescription
Lupin-Anthracnose_resistance-Alkemade-2022
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2022 Jan 05
Journal AbbreviationTheor Appl Genet
DOI10.1007/s00122-021-04014-7
Elocation10.1007/s00122-021-04014-7
Copyright© 2022. The Author(s).
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany