LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2

Overview
Library NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
Unique NameLIBEST_026413
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026413 Chickpea drought stressed cDNA SSH library AS2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO066371HO066371EST
HO066502HO066502EST
HO066559HO066559EST
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HO066788HO066788EST
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HO067004HO067004EST
HO067141HO067141EST
HO067178HO067178EST
HO066284HO066284EST
HO066455HO066455EST
HO066503HO066503EST
HO066631HO066631EST
HO066763HO066763EST
HO066910HO066910EST
HO067005HO067005EST
HO067140HO067140EST
HO067193HO067193EST
HO066315HO066315EST
HO066409HO066409EST
HO066456HO066456EST
HO066500HO066500EST
HO066571HO066571EST
HO066632HO066632EST
HO066760HO066760EST

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