LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1

Overview
Library NameLIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
Unique NameLIBEST_026412
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1
  • Cultivar: ICC4958
  • Tissue: Shoot
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
HO066268HO066268EST
HO066267HO066267EST
HO066265HO066265EST
HO066264HO066264EST
HO066262HO066262EST
HO066261HO066261EST
HO066260HO066260EST
HO066259HO066259EST
HO066257HO066257EST
HO066256HO066256EST
HO066254HO066254EST
HO066253HO066253EST
HO066252HO066252EST
HO066251HO066251EST
HO066250HO066250EST
HO066249HO066249EST
HO066248HO066248EST
HO066247HO066247EST
HO066246HO066246EST
HO066245HO066245EST
HO066244HO066244EST
HO066243HO066243EST
HO066242HO066242EST
HO066241HO066241EST
HO066240HO066240EST

Pages