LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2

Overview
Library NameLIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
Unique NameLIBEST_026411
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2
  • Cultivar: ICC4958
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
Features
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Feature NameUnique NameType
HO064845HO064845EST
HO064844HO064844EST
HO064842HO064842EST
HO064840HO064840EST
HO064839HO064839EST
HO064838HO064838EST
HO064837HO064837EST
HO064835HO064835EST
HO064834HO064834EST
HO064833HO064833EST
HO064832HO064832EST
HO064831HO064831EST
HO064830HO064830EST
HO064829HO064829EST
HO064828HO064828EST
HO064827HO064827EST
HO064826HO064826EST
HO064824HO064824EST
HO064823HO064823EST
HO064821HO064821EST
HO064820HO064820EST
HO064819HO064819EST
HO064818HO064818EST
HO064817HO064817EST
HO064815HO064815EST

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