LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2

Overview
Library NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
Unique NameLIBEST_026415
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
  • Cultivar: RILs derived from cross between ICC4958 and ICC1882
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
HO068056HO068056EST
HO068055HO068055EST
HO068054HO068054EST
HO068053HO068053EST
HO068052HO068052EST
HO068050HO068050EST
HO068049HO068049EST
HO068048HO068048EST
HO068047HO068047EST
HO068046HO068046EST
HO068045HO068045EST
HO068044HO068044EST
HO068043HO068043EST
HO068042HO068042EST
HO068041HO068041EST
HO068040HO068040EST
HO068039HO068039EST
HO068038HO068038EST
HO068036HO068036EST
HO068035HO068035EST
HO068034HO068034EST
HO068033HO068033EST
HO068032HO068032EST
HO068031HO068031EST
HO068030HO068030EST

Pages