LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2

Overview
Library NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
Unique NameLIBEST_026415
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
  • Cultivar: RILs derived from cross between ICC4958 and ICC1882
  • Tissue: Root
  • Developmental stage: Flowering stage
  • Vector: pGEM-T Easy vector
  • Detail: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer‘s protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.
SNP Chip Base
Array NameLIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2
OrganismCicer arietinum (chickpea)
Typecdna_library
Features
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Feature NameUnique NameType
HO067783HO067783EST
HO067866HO067866EST
HO067884HO067884EST
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HO068001HO068001EST
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HO067720HO067720EST
HO067782HO067782EST
HO067834HO067834EST
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HO068039HO068039EST
HO067837HO067837EST
HO067842HO067842EST
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HO067886HO067886EST
HO067689HO067689EST
HO067722HO067722EST
HO067887HO067887EST
HO067960HO067960EST
HO068002HO068002EST
HO067725HO067725EST
HO067787HO067787EST
HO067831HO067831EST
HO067967HO067967EST
HO067989HO067989EST

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