A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum x C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5
Publication Overview
Abstract An integrated molecular marker map of the
chickpea genome was established using 130 recombinant
inbred lines from a wide cross between a cultivar resistant
to fusarium wilt caused by Fusarium oxysporum
Schlecht. emend. Snyd. &. Hans f. sp. ciceri (Padwick)
Snyd & Hans, and an accession of Cicer reticulatum (PI
489777), the wild progenitor of chickpea. A total of 354
markers were mapped on the RILs including 118
STMSs, 96 DAFs, 70 AFLPs, 37 ISSRs, 17 RAPDs,
eight isozymes, three cDNAs, two SCARs and three loci
that confer resistance against different races of fusarium
wilt. At a LOD-score of 4.0, 303 markers cover 2077.9
cM in eight large and eight small linkage groups at an
average distance of 6.8 cM between markers. Fifty one
markers (14.4%) were unlinked. A clustering of markers
in central regions of linkage groups was observed. Markers
of the same class, except for ISSR and RAPD markers,
tended to generate subclusters. Also, genes for resistance
to races 4 and 5 of fusarium wilt map to the same linkage
group that includes an STMS and a SCAR marker
previously shown to be linked to fusarium wilt race 1,
indicating a clustering of several fusarium-wilt resistance
genes around this locus. Significant deviation from the
expected 1 : 1 segregation ratio was observed for 136
markers (38.4%, P<0.05). Segregation was biased
towards the wild progenitor in 68% of the cases. Segregation
distortion was similar for all marker types except
for ISSRs that showed only 28.5% aberrant segregation.
The map is the most extended genetic map of chickpea
currently available. It may serve as a basis for markerassisted
selection and map-based cloning of fusarium
wilt resistance genes and other agronomically important
genes in future.
Features
This publication contains information about 34 features:
PagesProperties
Additional
details for this publication include:
|