|Library Name||LIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1 |
|Unique Name||LIBEST_017640 |
|Organism||Cicer arietinum (chickpea) |
|Type||cdna_library |LIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1
- Cultivar: WR-315
- Tissue: infected root
- Developmental stage: chickpea roots Post infection with Fusarium oxysporum f. sp. ciceri race 1
- Vector: pGEMT
- Detail: This sequence was obtained by cDNA-RAPD where cDNAs were synthesized from reverse transcription of mRNA isolated from chickpea roots at different days after infection with Fusarium oxysporum f. sp. ciceri Race 1. Three cDNA libraries were made using Zap-cDNA synthesis kit Gigapack III cloning Kit from Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037; 1) infected ressitant chickpea cultivar WR 315; 2) un-infected control of WR 315, 3) infected susceptable chickpea cultivar JG 62. cDNAs from the libraries were rescued by PCR amplification using flanking T3 and T7 promoter primers. Amplified cDNA was used as template to screen with RAPD primers from UBC. Small DNA fragments were generated, then amplified by PCR using different RAPD (hexamers) primers. The PCR products from all the three cDNA libraries were run on a 1.5% Agarose gel. The up-regulated bands form resistant infected library were isolated from the gel, reamplified using the same primers and cloned in pGEMT easy vector (Promega) and sequenced in both direction using the T7 and SP6 primer.
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