LIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1

Overview
Library NameLIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1
Unique NameLIBEST_017640
OrganismCicer arietinum (chickpea)
Typecdna_library
LIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1
  • Cultivar: WR-315
  • Tissue: infected root
  • Developmental stage: chickpea roots Post infection with Fusarium oxysporum f. sp. ciceri race 1
  • Vector: pGEMT
  • Detail: This sequence was obtained by cDNA-RAPD where cDNAs were synthesized from reverse transcription of mRNA isolated from chickpea roots at different days after infection with Fusarium oxysporum f. sp. ciceri Race 1. Three cDNA libraries were made using Zap-cDNA synthesis kit Gigapack III cloning Kit from Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037; 1) infected ressitant chickpea cultivar WR 315; 2) un-infected control of WR 315, 3) infected susceptable chickpea cultivar JG 62. cDNAs from the libraries were rescued by PCR amplification using flanking T3 and T7 promoter primers. Amplified cDNA was used as template to screen with RAPD primers from UBC. Small DNA fragments were generated, then amplified by PCR using different RAPD (hexamers) primers. The PCR products from all the three cDNA libraries were run on a 1.5% Agarose gel. The up-regulated bands form resistant infected library were isolated from the gel, reamplified using the same primers and cloned in pGEMT easy vector (Promega) and sequenced in both direction using the T7 and SP6 primer.
Features
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Feature NameUnique NameType
DR749499DR749499EST
DR749498DR749498EST
DR749501DR749501EST
DR749500DR749500EST
DR749502DR749502EST
DR749503DR749503EST
DR749504DR749504EST