QTL mapping and identification of genes associated with the resistance to Acanthoscelides obtectus in cultivated common bean using a high-density genetic linkage map.

Publication Overview
TitleQTL mapping and identification of genes associated with the resistance to Acanthoscelides obtectus in cultivated common bean using a high-density genetic linkage map.
AuthorsLi X, Tang Y, Wang L, Chang Y, Wu J, Wang S
TypeJournal Article
Journal NameBMC plant biology
Volume22
Issue1
Year2022
Page(s)260
CitationLi X, Tang Y, Wang L, Chang Y, Wu J, Wang S. QTL mapping and identification of genes associated with the resistance to Acanthoscelides obtectus in cultivated common bean using a high-density genetic linkage map.. BMC plant biology. 2022 May 25; 22(1):260.

Abstract

BACKGROUND
Common bean (Phaseolus vulgaris L.) is an important agricultural product with large nutritional value, and the insect pest Acanthoscelides obtectus (Say) seriously affects its product quality and commodity quality during storage. Few researches on genes of bruchid resistance have investigated in common bean cultivars.

RESULTS
In this study, a bruchid-resistant cultivar black kidney bean and a highly susceptible accession Longyundou3 from different gene banks were crossed to construct a recombinant inbred line population. The genetic analysis indicated a quantitative inheritance of the bruchid resistance trait controlled by polygenes. A high-density genetic map of a total map distance of 1283.68 cM with an average interval of 0.61 cM between each marker was constructed using an F6 population of 157 recombinant inbred lines. The map has 3106 bin markers, containing 2,234,769 SNPs. Using the high-density genetic map, a new quantitative trait locus for the resistance to Acanthoscelides obtectus was identified on chromosome 6. New molecular markers based on the candidate region were developed, and this locus was further delimited to an interval of 122.3 kb between SSR markers I6-4 and I6-16 using an F2 population. This region comprised five genes. Phvul.006G003700, which encodes a bifunctional inhibitor, may be a potential candidate gene for bruchid resistance. Sequencing analysis of candidate gene identified a 5 bp insertion-deletion in promoter of gene Phvul.006G003700 between two parents. Expression analysis of candidate gene revealed that the expression level of Phvul.006G003700 in bruchid-resistant parent was markedly higher than that in bruchid-susceptible parent both in dry seeds and leaves.

CONCLUSIONS
A high-density genetic linkage map was constructed utilizing whole-genome resequencing and one new QTL for bruchid resistance was identified on chromosome 6 in common bean cultivar. Phvul.006G003700 (encoding a bifunctional inhibitor) may be a potential candidate gene. These results may form the basis for further research to reveal the bruchid resistance molecular mechanism of common bean.

Features
This publication contains information about 37 features:
Feature NameUniquenameType
Bin1611Bin1611genetic_marker
Bin1578Bin1578genetic_marker
Bin1561Bin1561genetic_marker
Bin1565Bin1565genetic_marker
Bin1566Bin1566genetic_marker
Bin1570Bin1570genetic_marker
Bin1573Bin1573genetic_marker
Bin1624Bin1624genetic_marker
Bin1627Bin1627genetic_marker
Bin1630Bin1630genetic_marker
Bin1634Bin1634genetic_marker
Bin1642Bin1642genetic_marker
Bin1662Bin1662genetic_marker
Bin1686Bin1686genetic_marker
Bin1700Bin1700genetic_marker
Bin1712Bin1712genetic_marker
Bin1719Bin1719genetic_marker
Bin1727Bin1727genetic_marker
Bin1736Bin1736genetic_marker
Bin1745Bin1745genetic_marker
Bin1751Bin1751genetic_marker
I6-3I6-3genetic_marker
I6-4I6-4genetic_marker
I6-11I6-11genetic_marker
I6-13I6-13genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Bean-Bruchid_resistance-Li-2022
Featuremaps
This publication contains information about 2 maps:
Map Name
Bean-BKB/LYD3-RIL-F6
Bean-BKB/LYD3-RIL-F6-phys
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
BKBBlack kidney beanaccession
LYD3Longyundou3accession
BKB/LYD3-RIL-F6BKB/LYD3-RIL-F6population
Properties
Additional details for this publication include:
Property NameValue
LanguageEnglish
Publication ModelElectronic
ISSN1471-2229
eISSN1471-2229
Publication Date2022 May 25
Journal AbbreviationBMC Plant Biol
DOI10.1186/s12870-022-03635-4
Elocation10.1186/s12870-022-03635-4
Journal CountryEngland
Copyright© 2022. The Author(s).
Language Abbreng
Publication TypeJournal Article