Spatiotemporal Transcriptomics and Metabolic Profiling Provide Insights into Gene Regulatory Networks during Lentil Seed Development.

Publication Overview
TitleSpatiotemporal Transcriptomics and Metabolic Profiling Provide Insights into Gene Regulatory Networks during Lentil Seed Development.
AuthorsYu B, Gao P, Song J, Yang H, Qin L, Yu X, Song H, Coulson J, Bekkaoui Y, Akhov L, Han X, Cram D, Wei Y, Zaharia LI, Zou J, Konkin D, Quilichini TD, Fobert P, Patterson N, Datla R, Xiang D
TypeJournal Article
Journal NameThe Plant journal : for cell and molecular biology
Year2023
CitationYu B, Gao P, Song J, Yang H, Qin L, Yu X, Song H, Coulson J, Bekkaoui Y, Akhov L, Han X, Cram D, Wei Y, Zaharia LI, Zou J, Konkin D, Quilichini TD, Fobert P, Patterson N, Datla R, Xiang D. Spatiotemporal Transcriptomics and Metabolic Profiling Provide Insights into Gene Regulatory Networks during Lentil Seed Development.. The Plant journal : for cell and molecular biology. 2023 Mar 25.

Abstract

Lentil (Lens culinaris Medik.) is a nutritious legume, with seeds rich in protein, minerals and an array of diverse specialized metabolites. The formation of a seed requires regulation and tight coordination of developmental programs to form the embryo, endosperm and seed coat compartments, which determines the structure and composition of mature seed and thus its end-use quality. Understanding the molecular and cellular events and metabolic processes of seed development is essential for improving lentil yield and seed nutritional value. However, such information remains largely unknown, especially at the seed compartment level. In this study, we generated high resolution spatiotemporal gene expression profiles in lentil embryo, seed coat and whole seeds from fertilization through maturation. Apart from anatomical difference between embryo and seed coat, comparative transcriptomic analysis and weighted gene co-expression network analysis revealed embryo and seed coat specific genes and gene modules predominant in specific tissue and stage, which highlights distinct genetic programming. Furthermore, we investigated the dynamic profiles of flavonoid, isoflavone, phytic acid and saponin in different seed compartments across seed development. Coupled with transcriptome data, we identified sets of candidate genes involved in the biosynthesis of these metabolites. The global view of the transcriptional and metabolic changes of lentil seed tissues throughout development provides a valuable resource for dissecting the genetic control of secondary metabolism and development of molecular tools for improving seed nutritional quality.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1365-313X
eISSN1365-313X
Publication Date2023 Mar 25
Journal AbbreviationPlant J
DOI10.1111/tpj.16205
Elocation10.1111/tpj.16205
CopyrightThis article is protected by copyright. All rights reserved.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland