Discovery of EST-derived microsatellite primers in the legume Lens culinaris (Fabaceae)

Publication Overview
TitleDiscovery of EST-derived microsatellite primers in the legume Lens culinaris (Fabaceae)
AuthorsJain N, Dikshit HK, Singh D, Singh A, Kumar H
TypeJournal Article
Journal NameApplications in plant sciences
Volume1
Issue7
Year2013
CitationJain N, Dikshit HK, Singh D, Singh A, Kumar H. Discovery of EST-derived microsatellite primers in the legume Lens culinaris (Fabaceae). Applications in plant sciences. 2013 Jul; 1(7).

Abstract

UNLABELLED

PREMISE OF THE STUDY
We developed microsatellite markers in the legume Lens culinaris from publicly available databases to enrich the limited marker resource available for the crop. •

METHODS AND RESULTS
Eighty-two primer sets were identified using expressed sequence sets of L. culinaris available in the National Center for Biotechnology Information (NCBI) database and were characterized in six species of Lens. Among them, 20 simple sequence repeat (SSR) primers produced no amplification product, 43 produced monomorphic products, and 19 were polymorphic. The primers amplified mono-, di-, tri-, tetra-, penta-, and hexanucleotide repeats with one to four alleles. These SSR loci successfully amplified in five related wild species, with a total of 61 primer pairs in L. nigricans and L. odemensis (98.39%), 59 in L. tomentosus (95.1%), and 60 in L. ervoides and L. orientalis (96.7%), respectively. •

CONCLUSIONS
The microsatellite markers discovered in this study will be useful in genetic mapping, marker-assisted breeding, and characterization of germplasm.

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Pages

Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Journal CountryUnited States
ISSN2168-0450
eISSN2168-0450
Publication Date2013 Jul
Journal AbbreviationAppl Plant Sci
DOI10.3732/apps.1200539
Elocationapps.1200539
PIIapps.1200539
Publication ModelElectronic-eCollection
LanguageEnglish
Language Abbreng
Cross References
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DatabaseAccession
PMID: PubMedPMID:25202567