Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)

Publication Overview
TitleVariability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)
AuthorsGawłowska M, Lahuta L, Święcicki W, Krajewski P
TypeJournal Article
Journal NameCzech journal of genetics and plant breeding
Volume50
Issue2
Year2014
Page(s)157-162
CitationGawłowska M, Lahuta L, Święcicki W, Krajewski P. Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.). Czech journal of genetics and plant breeding. 2014; 50(2):157-162.

Abstract

Anti-nutritional compounds are among the obstacles to the use of pea seeds as a protein source in both feed and food. These compounds are poorly digested by both monogastric animals and humans. There are three main oligosaccharides in pea: raffinose, stachyose and verbascose (raffinose family oligosaccharides - RFOs). The concentration of oligosaccharides in dry seeds, the oligosaccharide percent to the total content of soluble sugars and quantitative trait loci (QTLs) were analysed in the mapping population Wt10245 × Wt11238. The composition and concentration of soluble carbohydrates in seeds harvested from two field experiments (2002 and 2004) were analysed by the high resolution gas chromatography method. The Wt10245 × Wt11238 population was chosen because of the greater difference in the concentration of RFOs in seeds between parental lines (56.48 mg/g seed in Wt10245 and 99.1 mg/g seed in Wt11238). The average levels of oligosaccharides (mg/g seed) from both field experiments in the mapping population were: myo-inositol 1.5, sucrose 33.3, galactinol 0.8, raffinose 9.6, stachyose 30.1, verbascose 37.1. The total oligosaccharide concentration was 76.8 mg/g seed. This comprised anaverage of 68% soluble sugars, with the range from 59% to 75%. There was no interaction between lines and years of experiments (significance of lines × year interaction, F statistic > 0.01). One main quantitative trait locus was found for both experiments in LG VA (the tl-r interval) and three additional: in LG I (five traits 2002 and 2004 near afp1k), LG II (two traits 2002 near afp15h) and LG IIIB (five traits 2004 and 2002 near afp4i and M16). The main QTL was responsible for the level of RFOs and the total soluble sugar concentration in seeds. The results are in agreement with the knowledge of RFO biosynthesis. This makes selection for changes in the proportion of the particular oligosaccharides difficult, like in Phaseolus. However, it is possible to decrease the RFO content in pea seeds. The linkage between QTL and the gene r is interesting. The rugosus (r) locus changes the morphology and distribution of starch grains, decreases the total starch accumulation, produces a higher ratio of amylose to amylopectin and higher sugar and water content during development along with changes in cell size and lipid content.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Wt10245Wt10245cultivar
Wt11238Wt11238cultivar
Wt10245_x_Wt11238Wt10245_x_Wt11238population
Features
This publication contains information about 34 features:
Feature NameUniquenameType
Myo-inositol contentqMYOICN.Wt10245xWt11238.LGI.2011QTL
Myo-inositol contentqMYOICN.Wt10245xWt11238.LGIIIB.2011QTL
Myo-inositol contentqMYOICN.Wt10245xWt11238.LGVA.2011QTL
Sucrose contentqSUCROSECN.Wt10245xWt11238.LGII.2011QTL
Sucrose contentqSUCROSECN.Wt10245xWt11238.LGVA.2011QTL
Sucrose contentqSUCROSECN.Wt10245xWt11238.LGVB.2011QTL
Raffinose contentqRAFFCN.Wt10245xWt11238.LGI.2011QTL
Raffinose contentqRAFFCN.Wt10245xWt11238.LGVA.2011QTL
Stachyose contentqSTACHCN.Wt10245xWt11238.LGI.2011QTL
Stachyose contentqSTACHCN.Wt10245xWt11238.LGII.2011QTL
Stachyose contentqSTACHCN.Wt10245xWt11238.LGIIIB.2011QTL
Stachyose contentqSTACHCN.Wt10245xWt11238.LGVA.2011.1QTL
Stachyose contentqSTACHCN.Wt10245xWt11238.LGVA.2011.2QTL
Verbascose contentqVERBACN.Wt10245xWt11238.LGIIIB.2011QTL
Verbascose contentqVERBACN.Wt10245xWt11238.LGIV.2011QTL
Verbascose contentqVERBACN.Wt10245xWt11238.LGVA.2011QTL
Raffinose family oligosaccharides contentqRFOCN.Wt10245xWt11238.LGI.2011QTL
Raffinose family oligosaccharides contentqRFOCN.Wt10245xWt11238.LGIIIB.2011QTL
Raffinose family oligosaccharides contentqRFOCN.Wt10245xWt11238.LGVA.2011.1QTL
Raffinose family oligosaccharides contentqRFOCN.Wt10245xWt11238.LGVA.2011.2QTL
Myo-inositol contentqMYOICN.Wt10245xWt11238.LGI.2012QTL
Myo-inositol contentqMYOICN.Wt10245xWt11238.LGVA.2012QTL
Sucrose contentqSUCROSECN.Wt10245xWt11238.LGVA.2012QTL
Galactinol concentqGALACCN.Wt10245xWt11238.LGVA.2012QTL
Galactinol concentqGALACCN.Wt10245xWt11238.LGVIA.2012QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Variability_Oligosaccharide_Concentration-Gawlowska-2014
Featuremaps
This publication contains information about 1 maps:
Map Name
Pea-Wt10245xWt11238-F2
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2014
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://www.cazv.cz/static.asp?str=genet_cz&ch=54&ids=763
KeywordsPhaseolus, Pisum sativum, amylopectin, amylose, biosynthesis, field experimentation, gas chromatography, genes, humans, lipid content, loci, myo-inositol, peas, quantitative trait loci, raffinose, seeds, stachyose, starch granules, sucrose, water content
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4828306