Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height

Publication Overview
TitleDevelopment of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height
AuthorsGupta S, Kumar T, Verma S, Bharadwaj C, Bhatia S
TypeJournal Article
Journal NameMolecular biology reports
Year2015
CitationGupta S, Kumar T, Verma S, Bharadwaj C, Bhatia S. Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height. 2015 Oct 7.

Abstract

Seed weight and plant height are important agronomic traits and contribute to seed yield. The objective of this study was to identify QTLs underlying these traits using an intra-specific mapping population of chickpea. A F11 population of 177 recombinant inbred lines derived from a cross between SBD377 (100-seed weight- 48 g and plant height- 53 cm) and BGD112 (100-seed weight- 15 g and plant height- 65 cm) was used. A total of 367 novel EST-derived functional markers were developed which included 187 EST-SSRs, 130 potential intron polymorphisms (PIPs) and 50 expressed sequence tag polymorphisms (ESTPs). Along with these, 590 previously published markers including 385 EST-based markers and 205 genomic SSRs were utilized. Of the 957 markers tested for analysis of parental polymorphism between the two parents of the mapping population, 135 (14.64 %) were found to be polymorphic. Of these, 131 polymorphic markers could be mapped to the 8 linkage groups. The linkage map had a total length of 1140.54 cM with an average marker density of 8.7 cM. The map was further used for QTL identification using composite interval mapping method (CIM). Two QTLs each for seed weight, qSW-1 and qSW-2 (explaining 11.54 and 19.24 % of phenotypic variance, respectively) and plant height, qPH-1 and qPH-2 (explaining 13.98 and 12.17 % of phenotypic variance, respectively) were detected. The novel set of genic markers, the intra-specific linkage map and the QTLs identified in the present study will serve as valuable genomic resources in improving the chickpea seed yield using marker-assisted selection (MAS) strategies.

Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
ICCV2ICCV2cultivar
SBD377SBD377cultivar
BGD112BGD112cultivar
SBD377_X_BGD112SBD377_X_BGD112population
Featuremaps
This publication contains information about 1 maps:
Map Name
Chickpea_SBD377_X_BGD112_RIL
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1573-4978
Publication Date2015 Oct 7
Journal AbbreviationMol. Biol. Rep.
eISSN1573-4978
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Cross References
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DatabaseAccession
PMID: PubMedPMID:26446030