Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height

Publication Overview
TitleDevelopment of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height
AuthorsGupta S, Kumar T, Verma S, Bharadwaj C, Bhatia S
TypeJournal Article
Journal NameMolecular biology reports
Year2015
CitationGupta S, Kumar T, Verma S, Bharadwaj C, Bhatia S. Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height. 2015 Oct 7.

Abstract

Seed weight and plant height are important agronomic traits and contribute to seed yield. The objective of this study was to identify QTLs underlying these traits using an intra-specific mapping population of chickpea. A F11 population of 177 recombinant inbred lines derived from a cross between SBD377 (100-seed weight- 48 g and plant height- 53 cm) and BGD112 (100-seed weight- 15 g and plant height- 65 cm) was used. A total of 367 novel EST-derived functional markers were developed which included 187 EST-SSRs, 130 potential intron polymorphisms (PIPs) and 50 expressed sequence tag polymorphisms (ESTPs). Along with these, 590 previously published markers including 385 EST-based markers and 205 genomic SSRs were utilized. Of the 957 markers tested for analysis of parental polymorphism between the two parents of the mapping population, 135 (14.64 %) were found to be polymorphic. Of these, 131 polymorphic markers could be mapped to the 8 linkage groups. The linkage map had a total length of 1140.54 cM with an average marker density of 8.7 cM. The map was further used for QTL identification using composite interval mapping method (CIM). Two QTLs each for seed weight, qSW-1 and qSW-2 (explaining 11.54 and 19.24 % of phenotypic variance, respectively) and plant height, qPH-1 and qPH-2 (explaining 13.98 and 12.17 % of phenotypic variance, respectively) were detected. The novel set of genic markers, the intra-specific linkage map and the QTLs identified in the present study will serve as valuable genomic resources in improving the chickpea seed yield using marker-assisted selection (MAS) strategies.

Features
This publication contains information about 135 features:
Feature NameUniquenameType
CESSR121_1CESSR121_1genetic_marker
PIP323PIP323genetic_marker
PIP335PIP335genetic_marker
CEST69_1CEST69_1genetic_marker
CEST195CEST195genetic_marker
PIP357PIP357genetic_marker
CESSR153_1CESSR153_1genetic_marker
CESSR207CESSR207genetic_marker
CESSR260CESSR260genetic_marker
PIP155_1PIP155_1genetic_marker
CESSR253CESSR253genetic_marker
CESSR138_1CESSR138_1genetic_marker
PIP151_1PIP151_1genetic_marker
PIP321PIP321genetic_marker
CEST48_1CEST48_1genetic_marker
TA113TA113genetic_marker
PIP148_1PIP148_1genetic_marker
CEST133_1CEST133_1genetic_marker
PIP147_1PIP147_1genetic_marker
NCPGR251NCPGR251genetic_marker
CESSR159_1CESSR159_1genetic_marker
TA59TA59genetic_marker
PIP194_1PIP194_1genetic_marker
TS82TS82genetic_marker
CEST184CEST184genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Chickpea_SBD377_X_BGD112_RIL
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
ICCV2ICCV2accession
SBD377SBD377accession
BGD112BGD112accession
SBD377_X_BGD112SBD377_X_BGD112population
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1573-4978
Publication Date2015 Oct 7
Journal AbbreviationMol. Biol. Rep.
eISSN1573-4978
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article