Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species

Publication Overview
TitleUse of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species
AuthorsSingh A, Dikshit HK, Singh D, Jain N, Aski M, Sarker A, Sharma TR
TypeJournal Article
Journal NameJournal of Agricultural Science
Year2016
Page(s)1-16
CitationSingh A, Dikshit HK, Singh D, Jain N, Aski M, Sarker A, Sharma TR. Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species. 2016; 1-16.

Abstract

Expressed sequence tag-simple sequence repeat (EST-SSR) markers were used to analyse genetic diversity among three Lens species. The SSR loci amplified successfully in wild species, with 94·82% transferability in Lens culinaris subsp. orientalis, 95·4% in Lens nigricans, 98·81% in L. culinaris subsp. odemensis, 94·82% in L. culinaris subsp. tomentosus and 96·55% in Lens ervoides. Ninety-nine alleles (average 3·41 alleles/locus) were detected by 29 SSR markers. Based on the unweighted pair group method with arithmetic mean cluster analysis, all the genotypes were grouped into three clusters at a similarity level of 0·30. The diversity analysis indicated no species-specific clustering of the wild and cultivated species. Wild species L. nigricans and L. culinaris subsp. odemensis, L. culinaris subsp. orientalis and L. ervoides were grouped in Cluster I, whereas the Mediterranean land races of L. culinaris subsp. culinaris and L. culinaris subsp. tomentosus formed a separate group in Cluster II A. Cluster II B comprised L. ervoides, L. culinaris subsp. orientalis and L. culinaris subsp. culinaris. Clusters II C, II D and II F included cultivated Indian lentil genotypes. Cluster II E comprised Indian and Mediterranean germplasm lines. Cluster II F included three early maturing germplasm lines, whereas Cluster III included only two germplasm lines. The functional annotation of SSR-containing unigenes revealed that a majority of genes were involved in an important transport-related function or were a component of metabolic pathways. A high level of polymorphism of EST-SSRs and their transferability to related wild species indicated that these markers could be used for molecular screening, map construction, comparative genomic studies and marker-assisted selection.
Stocks
This publication contains information about 26 stocks:
Stock NameUniquenameType
FLIP20047LFLIP20047Lcultivar
IG-70208IG-70208cultivar
IG-70211IG-70211cultivar
IG-112IG-112cultivar
IG-115IG-115cultivar
IG-125IG-125cultivar
IG-208IG-208cultivar
IG-69502IG-69502cultivar
IG-69513IG-69513cultivar
IG-69517IG-69517cultivar
IG-69522IG-69522cultivar
G-70174G-70174cultivar
ILWL-7ILWL-7cultivar
ILWL-81ILWL-81cultivar
ILWL-254ILWL-254cultivar
ILWL-35ILWL-35cultivar
ILWL-153ILWL-153cultivar
ILWL-91ILWL-91cultivar
ILWL-93ILWL-93cultivar
ILWL-126ILWL-126cultivar
ILWL-206ILWL-206cultivar
ILWL-139ILWL-139cultivar
ILWL-393ILWL-393cultivar
ILWL-111ILWL-111cultivar
ILWL-22ILWL-22cultivar

Pages

Features
This publication contains information about 29 features:
Feature NameUniquenameType
PLC4PLC4genetic_marker
PLC5PLC5genetic_marker
PLC10PLC10genetic_marker
PLC16PLC16genetic_marker
PLC17PLC17genetic_marker
PLC30PLC30genetic_marker
PLC35PLC35genetic_marker
PLC38PLC38genetic_marker
PLC39PLC39genetic_marker
PLC42PLC42genetic_marker
PLC46PLC46genetic_marker
PLC51PLC51genetic_marker
PLC60PLC60genetic_marker
PLC63PLC63genetic_marker
PLC74PLC74genetic_marker
PLC81PLC81genetic_marker
PLC82PLC82genetic_marker
PLC83PLC83genetic_marker
PLC84PLC84genetic_marker
PLC88PLC88genetic_marker
PLC91PLC91genetic_marker
PLC95PLC95genetic_marker
PLC96PLC96genetic_marker
PLC98PLC98genetic_marker
PLC99PLC99genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
DOI10.1017/S0021859615001252
URLhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=10117856&fileId=S0021859615001252