GR916564

EST Overview


Name	GR916564
Unique Name	GR916564
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	249

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_ORYSJ (Ubiquitin-fold modifier 1 OS=Oryza sativa subsp. japonica GN=Os01g0962400 PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.325e-20
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   51 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 97

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_ARATH (Ubiquitin-fold modifier 1 OS=Arabidopsis thaliana GN=At1g77710 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 3.036e-20
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDG+GINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   41 EEFKVPPQTSAIITNDGIGINPQQSAGNVFLKHGSELRLIPRDRVGA 87

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_CHLIN (Ubiquitin-fold modifier 1 OS=Chlamydomonas incerta GN=PR46a PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.570e-19
Identity = 45/50 (90.00%), Postives = 47/50 (94.00%), Query Frame = -1

Query:  100 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASEM 249
            EEFKVP QTSAIITNDGVGINPQQ+AGNVFLKHGSELRLIPRDRVG S +
Sbjct:   48 EEFKVPAQTSAIITNDGVGINPQQTAGNVFLKHGSELRLIPRDRVGGSSL 97

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_CHLRE (Ubiquitin-fold modifier 1 OS=Chlamydomonas reinhardtii GN=PR46a PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.842e-18
Identity = 44/46 (95.65%), Postives = 44/46 (95.65%), Query Frame = -1

Query:  112 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVG 249
            EEFKVP QTSAIITNDGVGINPQQ AGNVFLKHGSELRLIPRDRVG
Sbjct:   45 EEFKVPAQTSAIITNDGVGINPQQIAGNVFLKHGSELRLIPRDRVG 90

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_AEDAE (Ubiquitin-fold modifier 1 OS=Aedes aegypti GN=AAEL006251 PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.842e-18
Identity = 42/47 (89.36%), Postives = 46/47 (97.87%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPP TSAIIT+DG+GINPQQ+AGNVFLKHGSELRLIPRDRVG+
Sbjct:   39 EEFKVPPATSAIITDDGIGINPQQTAGNVFLKHGSELRLIPRDRVGS 85

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_DROGR (Ubiquitin-fold modifier 1 OS=Drosophila grimshawi GN=GH22653 PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.331e-18
Identity = 41/46 (89.13%), Postives = 46/46 (100.00%), Query Frame = -1

Query:  112 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVG 249
            EEFKVPP+TSAIIT+DG+GI+PQQ+AGNVFLKHGSELRLIPRDRVG
Sbjct:   38 EEFKVPPETSAIITDDGIGISPQQTAGNVFLKHGSELRLIPRDRVG 83

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_BRUMA (Ubiquitin-fold modifier 1 OS=Brugia malayi GN=Bm1_46275 PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.080e-17
Identity = 43/49 (87.76%), Postives = 44/49 (89.80%), Query Frame = -1

Query:  103 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE 249
            EEFKVP  TSAIITNDGVGINP QSAGN+FLKHGSELRLIPRDRVG  E
Sbjct:   42 EEFKVPAATSAIITNDGVGINPTQSAGNIFLKHGSELRLIPRDRVGYCE 90

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_CAEBR (Ubiquitin-fold modifier 1 OS=Caenorhabditis briggsae GN=CBG12253 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.842e-17
Identity = 42/46 (91.30%), Postives = 43/46 (93.48%), Query Frame = -1

Query:  112 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVG 249
            EEFKVP  TSAIITNDGVGINP QSAGN+FLKHGSELRLIPRDRVG
Sbjct:   46 EEFKVPAATSAIITNDGVGINPAQSAGNIFLKHGSELRLIPRDRVG 91

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_ANOGA (Ubiquitin-fold modifier 1 OS=Anopheles gambiae GN=AGAP001364 PE=3 SV=3)

HSP 1 Score: 87.8113 bits (216), Expect = 1.842e-17
Identity = 41/46 (89.13%), Postives = 44/46 (95.65%), Query Frame = -1

Query:  112 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVG 249
            EEFKVPP TSAIIT+DG+GINPQQ+AG VFLKHGSELRLIPRDRVG
Sbjct:   40 EEFKVPPATSAIITDDGIGINPQQTAGTVFLKHGSELRLIPRDRVG 85

[Close]

BLAST of GR916564 vs. SwissProt
Match: UFM1_COLLI (Ubiquitin-fold modifier 1 OS=Columba livia GN=UFM1 PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.406e-17
Identity = 41/48 (85.42%), Postives = 45/48 (93.75%), Query Frame = -1

Query:  106 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGAS 249
            EEFKVP  TSAIITNDG+GINP Q+AGNVFLKHGS+LRLIPRDRVG+S
Sbjct:   38 EEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSDLRLIPRDRVGSS 85

[Close]

BLAST of GR916564 vs. TrEMBL
Match: A9PC65_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816297 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.386e-20
Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = -1

Query:  103 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE
Sbjct:   48 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE 96

[Close]

BLAST of GR916564 vs. TrEMBL
Match: B9S1Q5_RICCO (Ubiquitin-fold modifier 1, putative OS=Ricinus communis GN=RCOM_0867250 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.177e-19
Identity = 47/49 (95.92%), Postives = 49/49 (100.00%), Query Frame = -1

Query:  103 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA++
Sbjct:   44 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGATQ 92

[Close]

BLAST of GR916564 vs. TrEMBL
Match: C5XIN6_SORBI (Putative uncharacterized protein Sb03g046730 OS=Sorghum bicolor GN=Sb03g046730 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.713e-19
Identity = 47/48 (97.92%), Postives = 48/48 (100.00%), Query Frame = -1

Query:  106 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGAS 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA+
Sbjct:   49 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGAA 96

[Close]

BLAST of GR916564 vs. TrEMBL
Match: Q0JFT6_ORYSJ (Os01g0962400 protein OS=Oryza sativa subsp. japonica GN=Os01g0962400 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   51 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 97

[Close]

BLAST of GR916564 vs. TrEMBL
Match: D7KUL9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_895660 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 46/48 (95.83%), Postives = 48/48 (100.00%), Query Frame = -1

Query:  106 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGAS 249
            EEFKVPPQTSAIITNDG+GINPQQSAGNVFLKHGSELRLIPRDRVGA+
Sbjct:   42 EEFKVPPQTSAIITNDGIGINPQQSAGNVFLKHGSELRLIPRDRVGAA 89

[Close]

BLAST of GR916564 vs. TrEMBL
Match: C6SXP4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   41 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 87

[Close]

BLAST of GR916564 vs. TrEMBL
Match: B9H7B5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_716608 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/49 (95.92%), Postives = 48/49 (97.96%), Query Frame = -1

Query:  103 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASE 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHG+ELRLIPRDRVGA E
Sbjct:   48 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGTELRLIPRDRVGAFE 96

[Close]

BLAST of GR916564 vs. TrEMBL
Match: B6SXA8_MAIZE (Ubiquitin-fold modifier 1 OS=Zea mays PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/50 (94.00%), Postives = 48/50 (96.00%), Query Frame = -1

Query:  100 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGASEM 249
            EEFKVPPQTSAIITNDG+GINPQQSAGNVFLKHGSELRLIPRDRVGA  M
Sbjct:   46 EEFKVPPQTSAIITNDGIGINPQQSAGNVFLKHGSELRLIPRDRVGAVVM 95

[Close]

BLAST of GR916564 vs. TrEMBL
Match: A3A1S5_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_04854 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   36 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 82

[Close]

BLAST of GR916564 vs. TrEMBL
Match: A2WZD3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_05311 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.849e-19
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   51 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 97

[Close]

BLAST of GR916564 vs. TAIR peptide
Match: AT1G77710.1 (| Symbols: | CONTAINS InterPro DOMAIN/s: Ubiquitin-like, Ufm1 (InterPro:IPR005375); Has 244 Blast hits to 244 proteins in 106 species: Archae - 0; Bacteria - 0; Metazoa - 149; Fungi - 0; Plants - 47; Viruses - 0; Other Eukaryotes - 48 (source: NCBI BLink). | chr1:29206750-29207895 FORWARD LENGTH=93)

HSP 1 Score: 97.0561 bits (240), Expect = 2.459e-21
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = -1

Query:  109 EEFKVPPQTSAIITNDGVGINPQQSAGNVFLKHGSELRLIPRDRVGA 249
            EEFKVPPQTSAIITNDG+GINPQQSAGNVFLKHGSELRLIPRDRVGA
Sbjct:   41 EEFKVPPQTSAIITNDGIGINPQQSAGNVFLKHGSELRLIPRDRVGA 87

The following BLAST results are available for this feature:

BLAST of GR916564 vs. SwissProt
BLAST of GR916564 vs. TrEMBL
BLAST of GR916564 vs. TAIR peptide

BLAST of GR916564 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
UFM1_ORYSJ	2.325e-20	100.00	Ubiquitin-fold modifier 1 OS=Oryza sativa subsp. j...	[more]
UFM1_ARATH	3.036e-20	97.87	Ubiquitin-fold modifier 1 OS=Arabidopsis thaliana ...	[more]
UFM1_CHLIN	2.570e-19	90.00	Ubiquitin-fold modifier 1 OS=Chlamydomonas incerta...	[more]
UFM1_CHLRE	2.842e-18	95.65	Ubiquitin-fold modifier 1 OS=Chlamydomonas reinhar...	[more]
UFM1_AEDAE	2.842e-18	89.36	Ubiquitin-fold modifier 1 OS=Aedes aegypti GN=AAEL...	[more]
UFM1_DROGR	6.331e-18	89.13	Ubiquitin-fold modifier 1 OS=Drosophila grimshawi ...	[more]
UFM1_BRUMA	1.080e-17	87.76	Ubiquitin-fold modifier 1 OS=Brugia malayi GN=Bm1_...	[more]
UFM1_CAEBR	1.842e-17	91.30	Ubiquitin-fold modifier 1 OS=Caenorhabditis briggs...	[more]
UFM1_ANOGA	1.842e-17	89.13	Ubiquitin-fold modifier 1 OS=Anopheles gambiae GN=...	[more]
UFM1_COLLI	2.406e-17	85.42	Ubiquitin-fold modifier 1 OS=Columba livia GN=UFM1...	[more]

BLAST of GR916564 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A9PC65_POPTR	4.386e-20	100.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S1Q5_RICCO	2.177e-19	95.92	Ubiquitin-fold modifier 1, putative OS=Ricinus com...	[more]
C5XIN6_SORBI	3.713e-19	97.92	Putative uncharacterized protein Sb03g046730 OS=So...	[more]
Q0JFT6_ORYSJ	4.849e-19	100.00	Os01g0962400 protein OS=Oryza sativa subsp. japoni...	[more]
D7KUL9_ARALY	4.849e-19	95.83	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C6SXP4_SOYBN	4.849e-19	100.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9H7B5_POPTR	4.849e-19	95.92	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B6SXA8_MAIZE	4.849e-19	94.00	Ubiquitin-fold modifier 1 OS=Zea mays PE=4 SV=1	[more]
A3A1S5_ORYSJ	4.849e-19	100.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2WZD3_ORYSI	4.849e-19	100.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GR916564 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G77710.1	2.459e-21	97.87	\| Symbols: \| CONTAINS InterPro DOMAIN/s: Ubiquiti...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005375	Ubiquitin-like, Ufm1	PFAM	PF03671	Ufm1	coord: 1..40 score: 8.5
None	No IPR available	GENE3D	3.10.20.90	no description	coord: 1..47 score: 8.6
None	No IPR available	SUPERFAMILY	54236	Ubiquitin-like	coord: 1..48 score: 4.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916564 ID=GR916564; Name=GR916564; organism=Cicer arietinum; type=EST; length=249bp
GATTAGCGTGGTCGCGGCCGAGGTACTGGTGTTTATTCAACTACAACAGT
CAAAATAGATAGATAGATAGATAATGTGGTCAACTCAAACTTCTATACAC
ATCTCAGATGCACCAACTCTATCACGCGGAATCAGACGAAGCTCTGAACC
ATGCTTAAGGAAGACATTTCCTGCACTCTGTTGAGGATTTATCCCGACAC
CATCATTGGTGATAATAGCGCTTGTCTGTGGAGGAACCTTGAATTCTTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR005375	UFM1

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