GR916662

EST Overview


Name	GR916662
Unique Name	GR916662
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	151

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJH1_ALLPO (DnaJ protein homolog 1 (Fragment) OS=Allium porrum GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.334e-12
Identity = 30/41 (73.17%), Postives = 35/41 (85.37%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ DDL+  H LSLTEALCGFQF +THLDGRQLL+K
Sbjct:  241 HPKFQRKGDDLFYKHTLSLTEALCGFQFVLTHLDGRQLLIK 281

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJH_CUCSA (DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.967e-12
Identity = 28/41 (68.29%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ DDL+++H LSL E+LCGFQF +THLDGRQLL+K
Sbjct:  260 HPKFKRKGDDLFVEHTLSLVESLCGFQFILTHLDGRQLLIK 300

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJH2_ALLPO (DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.967e-12
Identity = 29/41 (70.73%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ DDL+ +H+LSLTEALCGFQF +THLD RQLL+K
Sbjct:  262 HPKFKRKGDDLFYEHSLSLTEALCGFQFVLTHLDNRQLLIK 302

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNAJ3_ARATH (Chaperone protein dnaJ 3 OS=Arabidopsis thaliana GN=ATJ3 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 6.967e-12
Identity = 28/41 (68.29%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+++H LSLTEALCGFQF +THLDGR LL+K
Sbjct:  262 HPKFKRKGEDLFVEHTLSLTEALCGFQFVLTHLDGRSLLIK 302

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJH_ATRNU (DnaJ protein homolog ANJ1 OS=Atriplex nummularia PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.552e-11
Identity = 28/41 (68.29%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+ +H LSLTEALCGF+F +THLDGRQLL+K
Sbjct:  262 HPKFKRKGEDLFYEHTLSLTEALCGFRFVLTHLDGRQLLIK 302

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNAJ2_ARATH (Chaperone protein dnaJ 2 OS=Arabidopsis thaliana GN=ATJ2 PE=1 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 2.647e-11
Identity = 27/41 (65.85%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+++H +SLTEALCGFQF +THLD RQLL+K
Sbjct:  263 HPKFKRKGEDLFVEHTISLTEALCGFQFVLTHLDKRQLLIK 303

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJA2_HUMAN (DnaJ homolog subfamily A member 2 OS=Homo sapiens GN=DNAJA2 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.360e-7
Identity = 23/41 (56.10%), Postives = 32/41 (78.05%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R+ +DL++ + + L EALCGFQFT  HLDGRQ++VK
Sbjct:  257 HEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVK 297

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJA2_BOVIN (DnaJ homolog subfamily A member 2 OS=Bos taurus GN=DNAJA2 PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.360e-7
Identity = 23/41 (56.10%), Postives = 32/41 (78.05%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R+ +DL++ + + L EALCGFQFT  HLDGRQ++VK
Sbjct:  257 HEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVK 297

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJA2_RAT (DnaJ homolog subfamily A member 2 OS=Rattus norvegicus GN=Dnaja2 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.748e-7
Identity = 22/41 (53.66%), Postives = 31/41 (75.61%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R+ +DL++ + + L EALCGFQFT  HLD RQ++VK
Sbjct:  257 HEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDARQIVVK 297

[Close]

BLAST of GR916662 vs. SwissProt
Match: DNJA2_MOUSE (DnaJ homolog subfamily A member 2 OS=Mus musculus GN=Dnaja2 PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.748e-7
Identity = 22/41 (53.66%), Postives = 31/41 (75.61%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R+ +DL++ + + L EALCGFQFT  HLD RQ++VK
Sbjct:  257 HEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDARQIVVK 297

[Close]

BLAST of GR916662 vs. TrEMBL
Match: C6TN54_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.699e-14
Identity = 36/41 (87.80%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP FRRE DDLYIDHNLSLTEALCGFQF V HLDGRQLL+K
Sbjct:  263 HPKFRREQDDLYIDHNLSLTEALCGFQFAVKHLDGRQLLIK 303

[Close]

BLAST of GR916662 vs. TrEMBL
Match: B7FID9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.015e-14
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP FRRE DDL+I+HNLSLTEALCGFQF VTHLDGRQLLVK
Sbjct:  264 HPKFRRERDDLHIEHNLSLTEALCGFQFNVTHLDGRQLLVK 304

[Close]

BLAST of GR916662 vs. TrEMBL
Match: O24074_MEDSA (DnaJ-like protein OS=Medicago sativa GN=msj1 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.785e-13
Identity = 35/41 (85.37%), Postives = 38/41 (92.68%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP FRRE DDL+I+HNLSLT+ALCGFQF VTHLDGRQLLVK
Sbjct:  264 HPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLLVK 304

[Close]

BLAST of GR916662 vs. TrEMBL
Match: B9RQ46_RICCO (Chaperone protein dnaJ, putative OS=Ricinus communis GN=RCOM_0954960 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.511e-12
Identity = 32/41 (78.05%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ DDL +DHNLSLTEALCGFQF +THLDGRQLL+K
Sbjct:  262 HPKFKRKEDDLVVDHNLSLTEALCGFQFVLTHLDGRQLLIK 302

[Close]

BLAST of GR916662 vs. TrEMBL
Match: C5XRY7_SORBI (Putative uncharacterized protein Sb04g032970 OS=Sorghum bicolor GN=Sb04g032970 PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.578e-12
Identity = 30/41 (73.17%), Postives = 38/41 (92.68%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R++DDLY++H +SLTEALCGFQF +THLDGRQLL+K
Sbjct:  264 HPKFKRKYDDLYVEHTISLTEALCGFQFVLTHLDGRQLLIK 304

[Close]

BLAST of GR916662 vs. TrEMBL
Match: B4FJC3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.744e-12
Identity = 30/41 (73.17%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R +DDLY++H +SLTEALCGFQF +THLDGRQLL+K
Sbjct:  265 HPKFKRMYDDLYVEHTISLTEALCGFQFVLTHLDGRQLLIK 305

[Close]

BLAST of GR916662 vs. TrEMBL
Match: C5YDG6_SORBI (Putative uncharacterized protein Sb06g024520 OS=Sorghum bicolor GN=Sb06g024520 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.501e-12
Identity = 30/41 (73.17%), Postives = 38/41 (92.68%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R++DDL+I+H +SLTEALCGFQF +THLDGRQLL+K
Sbjct:  261 HPRFKRKYDDLFIEHTISLTEALCGFQFILTHLDGRQLLIK 301

[Close]

BLAST of GR916662 vs. TrEMBL
Match: Q9ZWK3_SALGI (DnaJ homolog OS=Salix gilgiana GN=SGJ3 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.280e-11
Identity = 30/41 (73.17%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ DDL+++H LSLTEALCGFQF +THLDGRQLL+K
Sbjct:  264 HPKFKRKGDDLFVEHTLSLTEALCGFQFVLTHLDGRQLLIK 304

[Close]

BLAST of GR916662 vs. TrEMBL
Match: D7SLV4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_21.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018226001 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.280e-11
Identity = 30/41 (73.17%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R++DDLY++H LSLTEALCGFQF +THLDGRQLL+K
Sbjct:  262 HAKFKRKYDDLYVEHTLSLTEALCGFQFALTHLDGRQLLIK 302

[Close]

BLAST of GR916662 vs. TrEMBL
Match: A5C8A7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005641 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.280e-11
Identity = 30/41 (73.17%), Postives = 37/41 (90.24%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            H  F+R++DDLY++H LSLTEALCGFQF +THLDGRQLL+K
Sbjct:  262 HAKFKRKYDDLYVEHTLSLTEALCGFQFALTHLDGRQLLIK 302

[Close]

BLAST of GR916662 vs. TAIR peptide
Match: AT3G44110.2 (| Symbols: ATJ3, ATJ | DNAJ homologue 3 | chr3:15869179-15871059 REVERSE LENGTH=343)

HSP 1 Score: 69.3218 bits (168), Expect = 5.338e-13
Identity = 28/41 (68.29%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+++H LSLTEALCGFQF +THLDGR LL+K
Sbjct:  262 HPKFKRKGEDLFVEHTLSLTEALCGFQFVLTHLDGRSLLIK 302

[Close]

BLAST of GR916662 vs. TAIR peptide
Match: AT3G44110.1 (| Symbols: ATJ3, ATJ | DNAJ homologue 3 | chr3:15869115-15871059 REVERSE LENGTH=420)

HSP 1 Score: 69.3218 bits (168), Expect = 5.338e-13
Identity = 28/41 (68.29%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+++H LSLTEALCGFQF +THLDGR LL+K
Sbjct:  262 HPKFKRKGEDLFVEHTLSLTEALCGFQFVLTHLDGRSLLIK 302

[Close]

BLAST of GR916662 vs. TAIR peptide
Match: AT5G22060.1 (| Symbols: ATJ2, J2 | DNAJ homologue 2 | chr5:7303798-7305668 REVERSE LENGTH=419)

HSP 1 Score: 67.3958 bits (163), Expect = 2.029e-12
Identity = 27/41 (65.85%), Postives = 36/41 (87.80%), Query Frame = 2

Query:   29 HPNFRREHDDLYIDHNLSLTEALCGFQFTVTHLDGRQLLVK 151
            HP F+R+ +DL+++H +SLTEALCGFQF +THLD RQLL+K
Sbjct:  263 HPKFKRKGEDLFVEHTISLTEALCGFQFVLTHLDKRQLLIK 303

The following BLAST results are available for this feature:

BLAST of GR916662 vs. SwissProt
BLAST of GR916662 vs. TrEMBL
BLAST of GR916662 vs. TAIR peptide

BLAST of GR916662 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DNJH1_ALLPO	5.334e-12	73.17	DnaJ protein homolog 1 (Fragment) OS=Allium porrum...	[more]
DNJH_CUCSA	6.967e-12	68.29	DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 P...	[more]
DNJH2_ALLPO	6.967e-12	70.73	DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE...	[more]
DNAJ3_ARATH	6.967e-12	68.29	Chaperone protein dnaJ 3 OS=Arabidopsis thaliana G...	[more]
DNJH_ATRNU	1.552e-11	68.29	DnaJ protein homolog ANJ1 OS=Atriplex nummularia P...	[more]
DNAJ2_ARATH	2.647e-11	65.85	Chaperone protein dnaJ 2 OS=Arabidopsis thaliana G...	[more]
DNJA2_HUMAN	1.360e-7	56.10	DnaJ homolog subfamily A member 2 OS=Homo sapiens ...	[more]
DNJA2_BOVIN	1.360e-7	56.10	DnaJ homolog subfamily A member 2 OS=Bos taurus GN...	[more]
DNJA2_RAT	6.748e-7	53.66	DnaJ homolog subfamily A member 2 OS=Rattus norveg...	[more]
DNJA2_MOUSE	6.748e-7	53.66	DnaJ homolog subfamily A member 2 OS=Mus musculus ...	[more]

BLAST of GR916662 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TN54_SOYBN	4.699e-14	87.80	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FID9_MEDTR	8.015e-14	87.80	Putative uncharacterized protein OS=Medicago trunc...	[more]
O24074_MEDSA	1.785e-13	85.37	DnaJ-like protein OS=Medicago sativa GN=msj1 PE=2 ...	[more]
B9RQ46_RICCO	1.511e-12	78.05	Chaperone protein dnaJ, putative OS=Ricinus commun...	[more]
C5XRY7_SORBI	2.578e-12	73.17	Putative uncharacterized protein Sb04g032970 OS=So...	[more]
B4FJC3_MAIZE	5.744e-12	73.17	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5YDG6_SORBI	7.501e-12	73.17	Putative uncharacterized protein Sb06g024520 OS=So...	[more]
Q9ZWK3_SALGI	1.280e-11	73.17	DnaJ homolog OS=Salix gilgiana GN=SGJ3 PE=2 SV=1	[more]
D7SLV4_VITVI	1.280e-11	73.17	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5C8A7_VITVI	1.280e-11	73.17	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of GR916662 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G44110.2	5.338e-13	68.29	\| Symbols: ATJ3, ATJ \| DNAJ homologue 3 \| chr3:158...	[more]
AT3G44110.1	5.338e-13	68.29	\| Symbols: ATJ3, ATJ \| DNAJ homologue 3 \| chr3:158...	[more]
AT5G22060.1	2.029e-12	65.85	\| Symbols: ATJ2, J2 \| DNAJ homologue 2 \| chr5:7303...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002939	Chaperone DnaJ, C-terminal	PFAM	PF01556	DnaJ_C	coord: 10..50 score: 2.3
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 12..50 score: 3.3
IPR015609	Molecular chaperone, heat shock protein, Hsp40, DnaJ	PANTHER	PTHR11821	DNAJ/HSP40	coord: 10..50 score: 1.2
None	No IPR available	GENE3D	2.60.260.20	no description	coord: 10..49 score: 2.7
None	No IPR available	PANTHER	PTHR11821:SF72	HSP40, SUBFAMILY A, MEMBERS 1,2,4	coord: 10..50 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916662 ID=GR916662; Name=GR916662; organism=Cicer arietinum; type=EST; length=151bp
GATTTCGAGCGGCCGCCCGGGCAGGTGGCATCCGAACTTCAGGAGGGAGC
ATGATGACCTCTATATTGATCACAATCTGAGCTTAACTGAGGCTCTATGT
GGCTTCCAGTTTACTGTCACACATCTTGATGGGAGGCAACTATTGGTCAA
A

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006457	protein folding

Vocabulary: **Molecular Function**
Term	Definition
GO:0051082	unfolded protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002939	DnaJ_C
IPR008971	HSP40/DnaJ_pept-bd
IPR015609	Molecular chaperone, heat shock protein, Hsp40, DnaJ

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