GR420460

EST Overview


Name	GR420460
Unique Name	GR420460
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	332

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR420460 vs. TrEMBL
Match: O64461_BRANA (Aluminum-induced OS=Brassica napus GN=bali PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.603e-12
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADDI   ELLKGACGKSLA      FYSTA+GGL  +ENPKNK
Sbjct:  168 FADDI---ELLKGACGKSLASFPQGCFYSTALGGLRSFENPKNK 208

HSP 2 Score: 34.2686 bits (77), Expect = 1.603e-12
Identity = 15/33 (45.45%), Postives = 23/33 (69.70%), Query Frame = -2

Query:   35 ITAVPANEEEIWGGNSFMSE*FLMNIGSQRIEL 133
            ITA+PA EEEIWG    + E   +++ S+RI++
Sbjct:  209 ITAIPAREEEIWGATFKVKEQQFLHMESERIKI 241

HSP 3 Score: 25.409 bits (54), Expect = 1.603e-12
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQEGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: O64438_9FABA (ARG10 OS=Vigna radiata PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 7.503e-12
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD    +LLKG+CGKSLA      FYSTAVGGL CYENPKNK
Sbjct:  168 FADDA---DLLKGSCGKSLASFPQGCFYSTAVGGLRCYENPKNK 208

HSP 2 Score: 29.261 bits (64), Expect = 7.503e-12
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPA EEEIWG
Sbjct:  209 ITAVPAEEEEIWG 221

HSP 3 Score: 25.7942 bits (55), Expect = 7.503e-12
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQAGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: C6TJH0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 7.505e-12
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD    +LLKG+CGKSLA      FYSTAVGGL CYENPKNK
Sbjct:  168 FADDA---DLLKGSCGKSLASFPQGCFYSTAVGGLRCYENPKNK 208

HSP 2 Score: 29.261 bits (64), Expect = 7.505e-12
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPA EEEIWG
Sbjct:  209 ITAVPAEEEEIWG 221

HSP 3 Score: 25.7942 bits (55), Expect = 7.505e-12
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQAGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: C6TEA4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 9.702e-12
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD    +LLKG+CGKSLA      FYSTAVGGL CYENPKNK
Sbjct:  168 FADDA---DLLKGSCGKSLASFPQGCFYSTAVGGLRCYENPKNK 208

HSP 2 Score: 28.8758 bits (63), Expect = 9.702e-12
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITA+PA EEEIWG
Sbjct:  209 ITAIPAEEEEIWG 221

HSP 3 Score: 25.7942 bits (55), Expect = 9.702e-12
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQAGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: Q6UK15_GOSHI (Al-induced protein OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 9.702e-12
Identity = 31/44 (70.45%), Postives = 32/44 (72.73%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FAD+    ELLKGACGKSLA      FYSTAVGGL  YENPKNK
Sbjct:  168 FADNA---ELLKGACGKSLASFPQGCFYSTAVGGLRSYENPKNK 208

HSP 2 Score: 29.261 bits (64), Expect = 9.702e-12
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPA EEEIWG
Sbjct:  209 ITAVPAEEEEIWG 221

HSP 3 Score: 28.8758 bits (63), Expect = 9.702e-12
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQFGKVPL+WG
Sbjct:  149 DQFGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: D7LYL7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_488799 PE=4 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.622e-11
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD+   ELLKGACGKSLA      +YSTA+GGL  +ENPKNK
Sbjct:  168 FADDV---ELLKGACGKSLASFPQGCYYSTALGGLRSFENPKNK 208

HSP 2 Score: 31.5722 bits (70), Expect = 1.622e-11
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPANEEEIWG
Sbjct:  209 ITAVPANEEEIWG 221

HSP 3 Score: 26.1794 bits (56), Expect = 1.622e-11
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQVGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: A8IXL5_BRACM (Aluminum-induced protein OS=Brassica campestris PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.847e-11
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADDI   ELLKGACGKSLA      FYSTA+GGL  +ENPKNK
Sbjct:  168 FADDI---ELLKGACGKSLASFPQGCFYSTALGGLRSFENPKNK 208

HSP 2 Score: 28.8758 bits (63), Expect = 5.847e-11
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITA+PA EEEIWG
Sbjct:  209 ITAIPAREEEIWG 221

HSP 3 Score: 25.409 bits (54), Expect = 5.847e-11
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQEGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: Q5MCR8_9ASTR (Aluminum-induced protein OS=Codonopsis lanceolata GN=AIP PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 5.847e-11
Identity = 30/44 (68.18%), Postives = 32/44 (72.73%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FA+D    +LLKGACGKSLA      FYSTAVG L CYENPKNK
Sbjct:  168 FANDA---DLLKGACGKSLASFPQGCFYSTAVGELRCYENPKNK 208

HSP 2 Score: 30.0314 bits (66), Expect = 5.847e-11
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = -2

Query:   74 ITAVPANEEEIWGGNSFMSE 133
            ITAVPA EEEIWG   FM E
Sbjct:  209 ITAVPATEEEIWGA-KFMVE 227

HSP 3 Score: 25.7942 bits (55), Expect = 5.847e-11
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQAGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: B4YYA6_THEHA (ST7-5-2 OS=Thellungiella halophila PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.262e-10
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD+   ELLKGACGKSLA      +YSTA+GGL  +ENPKNK
Sbjct:  168 FADDV---ELLKGACGKSLASFPQGCYYSTALGGLRSFENPKNK 208

HSP 2 Score: 28.8758 bits (63), Expect = 1.262e-10
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITA+PA EEEIWG
Sbjct:  209 ITAIPAKEEEIWG 221

HSP 3 Score: 25.7942 bits (55), Expect = 1.262e-10
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQAGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TrEMBL
Match: D3GC09_9ROSI (Al-induced protein OS=Jatropha curcas PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 2.105e-10
Identity = 29/44 (65.91%), Postives = 32/44 (72.73%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FAD+I   ELLKGACGKSLA      FYSTAVG L  +ENPK+K
Sbjct:  168 FADNI---ELLKGACGKSLASFPEGCFYSTAVGELRSFENPKSK 208

HSP 2 Score: 29.261 bits (64), Expect = 2.105e-10
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPA EEEIWG
Sbjct:  209 ITAVPAKEEEIWG 221

HSP 3 Score: 28.8758 bits (63), Expect = 2.105e-10
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQFGKVPL+WG
Sbjct:  149 DQFGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TAIR peptide
Match: AT5G19140.1 (| Symbols: ATAILP1, AILP1 | Aluminium induced protein with YGL and LRDR motifs | chr5:6423398-6425785 FORWARD LENGTH=234)

HSP 1 Score: 53.1434 bits (126), Expect = 8.137e-13
Identity = 28/44 (63.64%), Postives = 33/44 (75.00%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLA------FYSTAVGGLMCYENPKNK 276
            FADD+   +LLKGACGKSLA      +YSTA+GGL  +ENPKNK
Sbjct:  168 FADDV---DLLKGACGKSLASFPQGCYYSTALGGLRSFENPKNK 208

HSP 2 Score: 28.8758 bits (63), Expect = 8.137e-13
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPANE EIWG
Sbjct:  209 ITAVPANEGEIWG 221

HSP 3 Score: 26.1794 bits (56), Expect = 8.137e-13
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQVGKVPLYWG 159

[Close]

BLAST of GR420460 vs. TAIR peptide
Match: AT5G19140.2 (| Symbols: ATAILP1, AILP1 | Aluminium induced protein with YGL and LRDR motifs | chr5:6423398-6425785 FORWARD LENGTH=222)

HSP 1 Score: 39.2762 bits (90), Expect = 7.895e-9
Identity = 21/38 (55.26%), Postives = 26/38 (68.42%), Query Frame = -3

Query:  163 FADDIFPQELLKGACGKSLAFYSTAVGGLMCYENPKNK 276
            FADD+   +LLKG       +YSTA+GGL  +ENPKNK
Sbjct:  168 FADDV---DLLKGC------YYSTALGGLRSFENPKNK 196

HSP 2 Score: 28.8758 bits (63), Expect = 7.895e-9
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = -2

Query:   95 ITAVPANEEEIWG 133
            ITAVPANE EIWG
Sbjct:  197 ITAVPANEGEIWG 209

HSP 3 Score: 26.1794 bits (56), Expect = 7.895e-9
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = -1

Query:  279 DQFGKVPLFWG 311
            DQ GKVPL+WG
Sbjct:  149 DQVGKVPLYWG 159

The following BLAST results are available for this feature:

BLAST of GR420460 vs. TrEMBL
BLAST of GR420460 vs. TAIR peptide

BLAST of GR420460 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O64461_BRANA	1.603e-12	70.45	Aluminum-induced OS=Brassica napus GN=bali PE=2 SV...	[more]
O64438_9FABA	7.503e-12	70.45	ARG10 OS=Vigna radiata PE=2 SV=1	[more]
C6TJH0_SOYBN	7.505e-12	70.45	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TEA4_SOYBN	9.702e-12	70.45	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q6UK15_GOSHI	9.702e-12	70.45	Al-induced protein OS=Gossypium hirsutum PE=2 SV=1	[more]
D7LYL7_ARALY	1.622e-11	65.91	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A8IXL5_BRACM	5.847e-11	70.45	Aluminum-induced protein OS=Brassica campestris PE...	[more]
Q5MCR8_9ASTR	5.847e-11	68.18	Aluminum-induced protein OS=Codonopsis lanceolata ...	[more]
B4YYA6_THEHA	1.262e-10	65.91	ST7-5-2 OS=Thellungiella halophila PE=2 SV=1	[more]
D3GC09_9ROSI	2.105e-10	65.91	Al-induced protein OS=Jatropha curcas PE=2 SV=1	[more]

BLAST of GR420460 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G19140.1	8.137e-13	63.64	\| Symbols: ATAILP1, AILP1 \| Aluminium induced prot...	[more]
AT5G19140.2	7.895e-9	55.26	\| Symbols: ATAILP1, AILP1 \| Aluminium induced prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF12504	DUF3711	coord: 16..45 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420460 ID=GR420460; Name=GR420460; organism=Cicer arietinum; type=EST; length=332bp
GTCCTCCCCATGCATGTCTACAATACATTTCCTAAAGCTCTATTCTCTGG
CTGCCAATGTTCATCAAAAATCATTCACTCATAAAACTGTTGCCACCCCA
AATTTCTTCTTCATTAGCAGGGACTGCAGTAATACCTTCCACCTTGAAAG
TTGAAAACATCCTTTATTTTTAGGATTCTCATAACACATCAATCCTCCAA
CAGCAGTTGAATAAAAAGCAAGTGACTTGCCACAAGCACCTTTAAGCAAT
TCTTGAGGGAATATATCATCAGCAAATGTCCCCAAAATAGGGGCACCTTA
CCAAATTGATCTACATATCCATCAGCAGTTAT

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