HO065449

EST Overview


Name	HO065449
Unique Name	HO065449
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	328

Libraries


Library Name	Type
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO065449 vs. SwissProt
Match: HS21C_PEA (Small heat shock protein, chloroplastic OS=Pisum sativum GN=HSP21 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 8.441e-10
Identity = 37/74 (50.00%), Postives = 44/74 (59.46%), Query Frame = -1

Query:  107 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXIXXXXXXXXXXVXXVQIQ 328
            VE D+LVIKSD  +     +  WS K+ S Y+T  KLPDNC K+KVKAE K+GVL I          V  VQIQ
Sbjct:  160 VEDDVLVIKSDHREENGGEDC-WSRKSYSCYDTRLKLPDNCEKEKVKAELKDGVLYITIPKTKIERTVIDVQIQ 232

[Close]

BLAST of HO065449 vs. SwissProt
Match: HS25P_ARATH (25.3 kDa heat shock protein, chloroplastic OS=Arabidopsis thaliana GN=HSP25.3 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.189e-9
Identity = 35/74 (47.30%), Postives = 42/74 (56.76%), Query Frame = -1

Query:  107 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXIXXXXXXXXXXVXXVQIQ 328
            VE ++LVIK +  K        WS ++ SSY T  +LPDNC KDK+KAE KNGVL I          V  VQIQ
Sbjct:  156 VEDNVLVIKGEQKKEDSDDS--WSGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERKVIDVQIQ 227

[Close]

BLAST of HO065449 vs. SwissProt
Match: HS22C_SOYBN (Small heat shock protein, chloroplastic (Fragment) OS=Glycine max GN=HSP22 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.189e-9
Identity = 36/75 (48.00%), Postives = 42/75 (56.00%), Query Frame = -1

Query:  107 VEGDILVIKSDFX-KXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXIXXXXXXXXXXVXXVQIQ 328
            VE D+LVIK     +     +  WS +  SSY+T  KLPDNC KDKVKAE KNGVL I          V  VQ+Q
Sbjct:  107 VEDDMLVIKGGHKSEQEHGGDDSWSSRTYSSYDTRLKLPDNCEKDKVKAELKNGVLYITIPKTKVERKVIDVQVQ 181

[Close]

BLAST of HO065449 vs. SwissProt
Match: HS22C_PETHY (Small heat shock protein, chloroplastic OS=Petunia hybrida GN=HSP22 PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.715e-8
Identity = 31/57 (54.39%), Postives = 36/57 (63.16%), Query Frame = -1

Query:  158 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXI 328
            VE D+LVIK +  K     +  W  +N SSY+T   LPDN  KDKVKAE KNGVL I
Sbjct:  169 VEDDVLVIKGEHKKEESGKDDSWG-RNYSSYDTRLSLPDNVDKDKVKAELKNGVLLI 224

[Close]

BLAST of HO065449 vs. SwissProt
Match: HS21C_SOLLC (Small heat shock protein, chloroplastic OS=Solanum lycopersicum GN=HSP21 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.760e-7
Identity = 29/57 (50.88%), Postives = 35/57 (61.40%), Query Frame = -1

Query:  158 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXI 328
            VE D+LVIK +  K     +     +N SSY+T   LPDN  KDK+KAE KNGVL I
Sbjct:  162 VENDMLVIKGEHKKEEDGRDKHSWGRNYSSYDTRLSLPDNVVKDKIKAELKNGVLFI 218

[Close]

BLAST of HO065449 vs. TrEMBL
Match: B9I224_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1095126 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.332e-8
Identity = 36/74 (48.65%), Postives = 43/74 (58.11%), Query Frame = -1

Query:  107 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXIXXXXXXXXXXVXXVQIQ 328
            VE D+LVIK +  K     +  WS  + SSY+T  +LPDNC KDK+KAE KNGVL I          V  VQIQ
Sbjct:  166 VEDDVLVIKGEHKKEETGDDS-WSSSSVSSYDTRLRLPDNCGKDKIKAELKNGVLFINIPKTKVEPKVIDVQIQ 238

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLG5_9MAGN (Chloroplast small heat shock protein (Fragment) OS=Machilus thunbergii GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.332e-8
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  +     E  WS ++ SSYNT   LPDNC KDKVKAE KNGVL
Sbjct:   56 VEDDMLVIKGEHKEEKEGGEVSWSSRSSSSYNTRLLLPDNCEKDKVKAELKNGVL 110

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLF9_9MAGN (Chloroplast small heat shock protein (Fragment) OS=Persea japonica GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.332e-8
Identity = 32/55 (58.18%), Postives = 37/55 (67.27%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  +     E  WS ++ SSYNT   LPDNC KDKVKAE KNGVL
Sbjct:   56 VEDDMLVIKGEHKEEKEGGEVSWSSRSSSSYNTRLLLPDNCEKDKVKAELKNGVL 110

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A9QVH3_9FABA (Heat shock protein OS=Ammopiptanthus mongolicus GN=hsp PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.739e-8
Identity = 33/57 (57.89%), Postives = 37/57 (64.91%), Query Frame = -1

Query:  158 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXI 328
            VE D+LVIK D  K     +  WS K  SSY+T   +PDNC KDKVKAE KNGVL I
Sbjct:  161 VEDDVLVIKGDHKKEQGGDDS-WSSKTYSSYDTRLMIPDNCEKDKVKAELKNGVLYI 216

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLG2_9MAGN (Chloroplast small heat shock protein (Fragment) OS=Machilus mushaensis GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.739e-8
Identity = 31/55 (56.36%), Postives = 37/55 (67.27%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  +     E  WS ++ SSYNT   LPDNC KDK+KAE KNGVL
Sbjct:   56 VEDDMLVIKGEHKEEKEGGEESWSSRSSSSYNTRLLLPDNCEKDKIKAEFKNGVL 110

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLG1_9MAGN (Chloroplast small heat shock protein (Fragment) OS=Persea kusanoi GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.739e-8
Identity = 31/55 (56.36%), Postives = 37/55 (67.27%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  +     E  WS ++ SSYNT   LPDNC KDK+KAE KNGVL
Sbjct:   56 VEDDMLVIKGEHKEEKEGGEESWSSRSSSSYNTRLLLPDNCEKDKIKAELKNGVL 110

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLG3_9MAGN (Chloroplast small heat shock protein (Fragment) OS=Persea obovatifolia GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.271e-8
Identity = 30/55 (54.55%), Postives = 37/55 (67.27%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LV+K +  +     E  WS ++ SSYNT   LPDNC KDK+KAE KNGVL
Sbjct:   56 VEDDMLVVKGEHKEEKEGGEESWSSRSSSSYNTRLLLPDNCEKDKIKAELKNGVL 110

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLF8_RHOSS (Chloroplast small heat shock protein (Fragment) OS=Rhododendron simsii GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.271e-8
Identity = 32/55 (58.18%), Postives = 38/55 (69.09%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  K     +  WS K+ SSY+T  +LPDNC KDKVKAE KNGVL
Sbjct:   59 VEDDVLVIKGEHKKEGGGDDS-WSGKSYSSYDTRLRLPDNCEKDKVKAELKNGVL 112

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLF5_9ERIC (Chloroplast small heat shock protein (Fragment) OS=Rhododendron rubropilosum GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.271e-8
Identity = 32/55 (58.18%), Postives = 38/55 (69.09%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  K     +  WS K+ SSY+T  +LPDNC KDKVKAE KNGVL
Sbjct:   59 VEDDVLVIKGEHKKEGGGDDS-WSGKSYSSYDTRLRLPDNCEKDKVKAELKNGVL 112

[Close]

BLAST of HO065449 vs. TrEMBL
Match: A7WLF4_9ERIC (Chloroplast small heat shock protein (Fragment) OS=Rhododendron ovatum GN=cpsHSP PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.271e-8
Identity = 32/55 (58.18%), Postives = 38/55 (69.09%), Query Frame = -1

Query:  164 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVL 328
            VE D+LVIK +  K     +  WS K+ SSY+T  +LPDNC KDKVKAE KNGVL
Sbjct:   59 VEDDVLVIKGEHKKEGGGDDS-WSGKSYSSYDTRLRLPDNCEKDKVKAELKNGVL 112

[Close]

BLAST of HO065449 vs. TAIR peptide
Match: AT4G27670.1 (| Symbols: HSP21 | heat shock protein 21 | chr4:13819048-13819895 REVERSE LENGTH=227)

HSP 1 Score: 60.077 bits (144), Expect = 3.320e-10
Identity = 35/74 (47.30%), Postives = 42/74 (56.76%), Query Frame = -1

Query:  107 VEGDILVIKSDFXKXXXXXEXFWSXKNXSSYNTXXKLPDNCXKDKVKAEXKNGVLXIXXXXXXXXXXVXXVQIQ 328
            VE ++LVIK +  K        WS ++ SSY T  +LPDNC KDK+KAE KNGVL I          V  VQIQ
Sbjct:  156 VEDNVLVIKGEQKKEDSDDS--WSGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERKVIDVQIQ 227

The following BLAST results are available for this feature:

BLAST of HO065449 vs. SwissProt
BLAST of HO065449 vs. TrEMBL
BLAST of HO065449 vs. TAIR peptide

BLAST of HO065449 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
HS21C_PEA	8.441e-10	50.00	Small heat shock protein, chloroplastic OS=Pisum s...	[more]
HS25P_ARATH	4.189e-9	47.30	25.3 kDa heat shock protein, chloroplastic OS=Arab...	[more]
HS22C_SOYBN	4.189e-9	48.00	Small heat shock protein, chloroplastic (Fragment)...	[more]
HS22C_PETHY	2.715e-8	54.39	Small heat shock protein, chloroplastic OS=Petunia...	[more]
HS21C_SOLLC	1.760e-7	50.88	Small heat shock protein, chloroplastic OS=Solanum...	[more]

BLAST of HO065449 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9I224_POPTR	1.332e-8	48.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A7WLG5_9MAGN	1.332e-8	58.18	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLF9_9MAGN	1.332e-8	58.18	Chloroplast small heat shock protein (Fragment) OS...	[more]
A9QVH3_9FABA	1.739e-8	57.89	Heat shock protein OS=Ammopiptanthus mongolicus GN...	[more]
A7WLG2_9MAGN	1.739e-8	56.36	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLG1_9MAGN	1.739e-8	56.36	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLG3_9MAGN	2.271e-8	54.55	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLF8_RHOSS	2.271e-8	58.18	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLF5_9ERIC	2.271e-8	58.18	Chloroplast small heat shock protein (Fragment) OS...	[more]
A7WLF4_9ERIC	2.271e-8	58.18	Chloroplast small heat shock protein (Fragment) OS...	[more]

BLAST of HO065449 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G27670.1	3.320e-10	47.30	\| Symbols: HSP21 \| heat shock protein 21 \| chr4:13...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 22..29 score
None	No IPR available	GENE3D	2.60.40.790	no description	coord: 2..74 score: 1.8
None	No IPR available	PANTHER	PTHR11527	SMALL HEAT-SHOCK PROTEIN (HSP20) FAMILY	coord: 1..74 score: 5.4
None	No IPR available	PANTHER	PTHR11527:SF18	HEAT SHOCK PROTEIN 26	coord: 1..74 score: 5.4
None	No IPR available	SEG	seg	seg	coord: 15..36 score: NAcoord: 58..71 score
IPR002068	Heat shock protein Hsp20	PFAM	PF00011	HSP20	coord: 1..74 score: 5.1
IPR002068	Heat shock protein Hsp20	PROFILE	PS01031	HSP20	coord: 1..61 score: 10
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 1..75 score: 3.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO065449 ID=HO065449; Name=HO065449; organism=Cicer arietinum; type=EST; length=328bp
GGAGGAAGTAGAAATTTGTGAGACATTNATATAACATANTTAAAGTATCT
TATTCATTCTACACTTTATAGCNCANGATGACNCTANAAAAAGCCTTANN
GTGTCNCTGAATTTGAACNTNNANAACCTTACGTCNNACTTTGGGCTTAG
GAACNTTGATNTAAAGCACACCATTTTTCANCTCTGCCTTAACCTTATCC
TTCNCACAGTTATCAGGAAGCTTGANACNAGTATTATAAGAACTATNGTT
TTTTNTTGACCAAAAANCCTCTTCACNACTTTCCTCCTTANGAAAATCAC
TTTTTATGACAAGAATATCGCCCTCNAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR002068	A-crystallin/Hsp20_dom
IPR008978	HSP20-like_chaperone

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