EL585383

EST Overview


Name	EL585383
Unique Name	EL585383
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	342

Libraries


Library Name	Type
LIBEST_020862 Chickpea Insect-Induced Library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_ARATH (Pre-mRNA branch site p14-like protein OS=Arabidopsis thaliana GN=At5g12190 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 3.971e-28
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+G +K T+GTAFVVYED YDAK AVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKK EDEI ++Q  Y
Sbjct:   47 QIRIGCDKATKGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKY 117

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_DROME (Pre-mRNA branch site p14-like protein OS=Drosophila melanogaster GN=CG13298 PE=1 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.158e-19
Identity = 44/71 (61.97%), Postives = 53/71 (74.65%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIRVG   +TRGTAFVVYED +DAK A DHLSGFNV NRYL+VLYYQ  K  K+ D  KK++E+  ++  Y
Sbjct:   43 QIRVGNTPETRGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYQSNKAFKRVDMDKKQEELNNIKAKY 113

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_MOUSE (Pre-mRNA branch site protein p14 OS=Mus musculus GN=Sf3b14 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.506e-19
Identity = 42/71 (59.15%), Postives = 53/71 (74.65%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIRVG   +TRGTA+VVYED +DAK A DHLSGFNV NRYL+VLYY   +  +K D KKKE+++  ++  Y
Sbjct:   47 QIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKY 117

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_HUMAN (Pre-mRNA branch site protein p14 OS=Homo sapiens GN=SF3B14 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.506e-19
Identity = 42/71 (59.15%), Postives = 53/71 (74.65%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIRVG   +TRGTA+VVYED +DAK A DHLSGFNV NRYL+VLYY   +  +K D KKKE+++  ++  Y
Sbjct:   47 QIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKY 117

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_CAEEL (Pre-mRNA branch site p14-like protein OS=Caenorhabditis elegans GN=C50D2.5 PE=2 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 6.355e-18
Identity = 41/71 (57.75%), Postives = 53/71 (74.65%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIRVG   +TRGTAFVVYED +DAKTA +HLSG+NV+NRYL+VLYYQ  K  K+ D +K   ++  ++  Y
Sbjct:   47 QIRVGNTAETRGTAFVVYEDIFDAKTACEHLSGYNVSNRYLVVLYYQATKAWKRMDTEKARTKLEDIKERY 117

[Close]

BLAST of EL585383 vs. SwissProt
Match: PM14_SCHPO (Pre-mRNA branch site p14-like protein OS=Schizosaccharomyces pombe GN=SPBC29A3.07c PE=2 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 1.201e-8
Identity = 27/46 (58.70%), Postives = 34/46 (73.91%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYY 173
            QIR+G    TRGTAFVVYE+  DA+ A + LSG+N  +RYL+V YY
Sbjct:   40 QIRLGNTVQTRGTAFVVYENVQDARRACEKLSGYNFMDRYLVVHYY 85

[Close]

BLAST of EL585383 vs. TrEMBL
Match: B7FGI3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 9.322e-31
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKMSKKFDQKKKEDEIARMQ  Y
Sbjct:   47 QIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: A5AES3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_037629 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 3.542e-30
Identity = 64/71 (90.14%), Postives = 67/71 (94.37%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKMSKKFDQKKKEDE+A+MQ  Y
Sbjct:   47 QIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELAKMQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: B3TLZ0_ELAGV (RNA binding protein OS=Elaeis guineensis var. tenera PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.031e-29
Identity = 64/71 (90.14%), Postives = 66/71 (92.96%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKMSKK DQKKKEDE+ARMQ  Y
Sbjct:   47 QIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKVDQKKKEDELARMQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: B9SXX1_RICCO (Pre-mRNA branch site protein p14, putative OS=Ricinus communis GN=RCOM_0974800 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.346e-29
Identity = 63/71 (88.73%), Postives = 67/71 (94.37%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKMSKKFDQ+KKE+EIA+MQ  Y
Sbjct:   47 QIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQRKKEEEIAKMQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: B9GG60_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_706505 PE=4 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.296e-29
Identity = 63/71 (88.73%), Postives = 67/71 (94.37%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKM+KKFDQKKKE+EIA+MQ  Y
Sbjct:   48 QIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQPAKMNKKFDQKKKEEEIAKMQEKY 118

[Close]

BLAST of EL585383 vs. TrEMBL
Match: B9HNI9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_649901 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 3.917e-29
Identity = 62/71 (87.32%), Postives = 66/71 (92.96%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTA+VVYED YDAKTAVDHLSGFNVANRYLIVLYYQ AKMSKKFDQKKKE+EI +MQ  Y
Sbjct:   48 QIRIGTNKDTRGTAYVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEEEIVKMQEKY 118

[Close]

BLAST of EL585383 vs. TrEMBL
Match: D7M4G9_ARALY (RNA recognition motif-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_909193 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 8.166e-27
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+G +K T+GTAFVVYED YDAK AVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKK EDEI ++Q  Y
Sbjct:   47 QIRIGCDKATKGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: A9SBS3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_210582 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 8.166e-27
Identity = 57/71 (80.28%), Postives = 64/71 (90.14%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+GTNKDTRGTAFVVYED YDAK AVDHLSGFNVANRYLIVLYYQ AKMSKK DQ+KK++E+ ++Q  Y
Sbjct:   47 QIRIGTNKDTRGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQQAKMSKKVDQRKKDEELTKLQEKY 117

[Close]

BLAST of EL585383 vs. TrEMBL
Match: D5A9T1_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.393e-26
Identity = 57/71 (80.28%), Postives = 63/71 (88.73%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+G NKDTRGTAFVVYED YDAK AVDHLSGFNVANRYLIVLYYQ +KM+KK D KKKED++A+MQ  Y
Sbjct:   46 QIRIGVNKDTRGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQQSKMTKKVDHKKKEDDLAKMQEKY 116

[Close]

BLAST of EL585383 vs. TrEMBL
Match: C5WVZ7_SORBI (Putative uncharacterized protein Sb01g003790 OS=Sorghum bicolor GN=Sb01g003790 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.627e-25
Identity = 58/71 (81.69%), Postives = 61/71 (85.92%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+G  KDTRGTA+VVYED YDAK AVDHLSGFNVANRYLIVLYYQ AKMSKK D KKKEDEI R+Q  Y
Sbjct:   47 QIRLGNAKDTRGTAYVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQPAKMSKKSDVKKKEDEITRLQEKY 117

[Close]

BLAST of EL585383 vs. TAIR peptide
Match: AT5G12190.1 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr5:3940731-3941105 REVERSE LENGTH=124)

HSP 1 Score: 123.25 bits (308), Expect = 3.155e-29
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKKEDEIARMQXNY 248
            QIR+G +K T+GTAFVVYED YDAK AVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKK EDEI ++Q  Y
Sbjct:   47 QIRIGCDKATKGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKY 117

[Close]

BLAST of EL585383 vs. TAIR peptide
Match: AT2G14870.1 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr2:6391172-6391477 FORWARD LENGTH=101)

HSP 1 Score: 52.7582 bits (125), Expect = 5.237e-8
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 3

Query:   36 QIRVGTNKDTRGTAFVVYEDXYDAKTAVDHL 128
            Q+R+G  K T+GTAFVVYED YDAK AVDHL
Sbjct:   48 QVRIGCEKGTKGTAFVVYEDIYDAKKAVDHL 78

The following BLAST results are available for this feature:

BLAST of EL585383 vs. SwissProt
BLAST of EL585383 vs. TrEMBL
BLAST of EL585383 vs. TAIR peptide

BLAST of EL585383 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
PM14_ARATH	3.971e-28	81.69	Pre-mRNA branch site p14-like protein OS=Arabidops...	[more]
PM14_DROME	1.158e-19	61.97	Pre-mRNA branch site p14-like protein OS=Drosophil...	[more]
PM14_MOUSE	7.506e-19	59.15	Pre-mRNA branch site protein p14 OS=Mus musculus G...	[more]
PM14_HUMAN	7.506e-19	59.15	Pre-mRNA branch site protein p14 OS=Homo sapiens G...	[more]
PM14_CAEEL	6.355e-18	57.75	Pre-mRNA branch site p14-like protein OS=Caenorhab...	[more]
PM14_SCHPO	1.201e-8	58.70	Pre-mRNA branch site p14-like protein OS=Schizosac...	[more]

BLAST of EL585383 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FGI3_MEDTR	9.322e-31	92.96	Putative uncharacterized protein OS=Medicago trunc...	[more]
A5AES3_VITVI	3.542e-30	90.14	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B3TLZ0_ELAGV	1.031e-29	90.14	RNA binding protein OS=Elaeis guineensis var. tene...	[more]
B9SXX1_RICCO	1.346e-29	88.73	Pre-mRNA branch site protein p14, putative OS=Rici...	[more]
B9GG60_POPTR	2.296e-29	88.73	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HNI9_POPTR	3.917e-29	87.32	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7M4G9_ARALY	8.166e-27	81.69	RNA recognition motif-containing protein OS=Arabid...	[more]
A9SBS3_PHYPA	8.166e-27	80.28	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
D5A9T1_PICSI	1.393e-26	80.28	Putative uncharacterized protein OS=Picea sitchens...	[more]
C5WVZ7_SORBI	2.627e-25	81.69	Putative uncharacterized protein Sb01g003790 OS=So...	[more]

BLAST of EL585383 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G12190.1	3.155e-29	81.69	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT2G14870.1	5.237e-8	74.19	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 25..40 score: NAcoord: 46..61 score
None	No IPR available	PANTHER	PTHR22630	PRE-MRNA BRANCH SITE PROTEIN	coord: 12..82 score: 4.7
None	No IPR available	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 10..73 score: 1.9
None	No IPR available	SEG	seg	seg	coord: 27..40 score
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 17..52 score: 4.9
IPR012677	Nucleotide-binding, alpha-beta plait	GENE3D	3.30.70.330	no description	coord: 13..73 score: 3.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EL585383 ID=EL585383; Name=EL585383; organism=Cicer arietinum; type=EST; length=342bp
ACGATATATGTCTGGCAAATACGGTGCTATTCGGTCAGATCCGAGTCGGA
ACTAACAAAGACACGCGCGGCACCGCTTTTGTCGTTTACGAAGACNTTTA
CGACGCTAAAACTGCCGTCGATCATCTCTCTGGTTTCAATGTCGCTAATC
GATATCTCATCGTTCTTTATTATCAGCATGCTAAGATGAGTAAGAAGTTT
GATCAGAAGAAGAAGGAAGATGAAATTGCTCGTATGCAGGNAAATTACCT
CGGCNNNTATGCCGTGTAAGCANTTGANATTTANGTGCTGAATGANTTAT
TGTCTTATTTGCAAGTTATACANTATNTGTTTTATGCTCCAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0000166	nucleotide binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000504	RRM_dom
IPR012677	Nucleotide-bd_a/b_plait_sf

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