EL585376

EST Overview


Name	EL585376
Unique Name	EL585376
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	429

Libraries


Library Name	Type
LIBEST_020862 Chickpea Insect-Induced Library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of EL585376 vs. TrEMBL
Match: B9IHV7_POPTR (Folylpolyglutamate synthase OS=Populus trichocarpa GN=FPGS4 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.186e-25
Identity = 55/68 (80.88%), Postives = 61/68 (89.71%), Query Frame = 2

Query:    2 PPHEFLYKEASHCGPEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            P  EFLY++AS+C P +GY ACSAV PSLPLTI WLRDCVRENPSLRLQVLVTGSLHLVGDVLK++RR
Sbjct:  514 PHREFLYEDASNCSPSNGYLACSAVIPSLPLTIKWLRDCVRENPSLRLQVLVTGSLHLVGDVLKVIRR 581

[Close]

BLAST of EL585376 vs. TrEMBL
Match: B9H9H1_POPTR (Folylpolyglutamate synthase OS=Populus trichocarpa GN=FPGS1 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.641e-25
Identity = 55/68 (80.88%), Postives = 60/68 (88.24%), Query Frame = 2

Query:    2 PPHEFLYKEASHCGPEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            P  +FLY++AS+C P DGY ACSAV PSLPLTI WLR CVRENPSLRLQVLVTGSLHLVGDVLKL+RR
Sbjct:  521 PRRQFLYEDASNCSPTDGYLACSAVIPSLPLTIKWLRHCVRENPSLRLQVLVTGSLHLVGDVLKLIRR 588

[Close]

BLAST of EL585376 vs. TrEMBL
Match: D7TII9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033555001 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.732e-21
Identity = 51/68 (75.00%), Postives = 54/68 (79.41%), Query Frame = 2

Query:    2 PPHEFLYKEASHCGPEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PP EFLY+  S   P     + SAV PSLPLTI WLRDCV+ENPSLRLQVLVTGSLHLVGDVLKLLRR
Sbjct:  606 PPFEFLYENGSSGSPSSKCLSSSAVMPSLPLTIRWLRDCVKENPSLRLQVLVTGSLHLVGDVLKLLRR 673

[Close]

BLAST of EL585376 vs. TrEMBL
Match: C6T5X1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.959e-20
Identity = 50/68 (73.53%), Postives = 55/68 (80.88%), Query Frame = 2

Query:    2 PPHEFLYKEASHCGPEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PPHEFLY +AS       Y A SAV PSLPLTI WLRDCVR++PS RLQVLVTGSLHLVGDVLKLL++
Sbjct:   59 PPHEFLYDDASSGCHSHNYLARSAVIPSLPLTIKWLRDCVRDHPSTRLQVLVTGSLHLVGDVLKLLKK 126

[Close]

BLAST of EL585376 vs. TrEMBL
Match: Q9SR80_ARATH (Putative folylpolyglutamate synthetase OS=Arabidopsis thaliana GN=T22K18.1 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.270e-19
Identity = 49/69 (71.01%), Postives = 55/69 (79.71%), Query Frame = 2

Query:    2 PPHEFLYKEASHCG-PEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PPH+FL  +A  CG P       SAV PSLPLTINWLRDCVR NPSL+L+VLVTGSLHLVGDVL+LL+R
Sbjct:  374 PPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 442

[Close]

BLAST of EL585376 vs. TrEMBL
Match: Q8W038_ARATH (Folylpolyglutamate synthetase, mitochondrial isoform OS=Arabidopsis thaliana GN=dhfs/fpgs3 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.270e-19
Identity = 49/69 (71.01%), Postives = 55/69 (79.71%), Query Frame = 2

Query:    2 PPHEFLYKEASHCG-PEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PPH+FL  +A  CG P       SAV PSLPLTINWLRDCVR NPSL+L+VLVTGSLHLVGDVL+LL+R
Sbjct:  557 PPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 625

[Close]

BLAST of EL585376 vs. TrEMBL
Match: A2RVP0_ARATH (At3g10160 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.270e-19
Identity = 49/69 (71.01%), Postives = 55/69 (79.71%), Query Frame = 2

Query:    2 PPHEFLYKEASHCG-PEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PPH+FL  +A  CG P       SAV PSLPLTINWLRDCVR NPSL+L+VLVTGSLHLVGDVL+LL+R
Sbjct:  396 PPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 464

[Close]

BLAST of EL585376 vs. TrEMBL
Match: D7TLM3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016464001 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.744e-12
Identity = 34/46 (73.91%), Postives = 40/46 (86.96%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            S VFPSLPL I WLRD VR+N S+R QVLVTGSLHLVGDVL+L+++
Sbjct:  573 STVFPSLPLAIKWLRDSVRQNQSVRFQVLVTGSLHLVGDVLRLVKK 618

[Close]

BLAST of EL585376 vs. TrEMBL
Match: B9SE17_RICCO (Folylpolyglutamate synthase, putative OS=Ricinus communis GN=RCOM_1482420 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.835e-11
Identity = 33/46 (71.74%), Postives = 41/46 (89.13%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            SAVFPSLPL I WLRD V +N S+R+QVLVTGSLHLVGDVL+++++
Sbjct:  486 SAVFPSLPLAIKWLRDSVLQNRSVRIQVLVTGSLHLVGDVLRIVKK 531

[Close]

BLAST of EL585376 vs. TrEMBL
Match: B9HT54_POPTR (Folylpolyglutamate synthase OS=Populus trichocarpa GN=FPGS3 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.838e-10
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLR 202
            S VFPSLPL I WLR+ V++N S+R QVLVTGSLHLVGDVL+L++
Sbjct:  536 STVFPSLPLAIKWLRESVQQNQSVRYQVLVTGSLHLVGDVLRLVK 580

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT3G10160.1 (| Symbols: ATDFC, DFC, FPGS2 | DHFS-FPGS homolog C | chr3:3139588-3143949 REVERSE LENGTH=625)

HSP 1 Score: 99.3673 bits (246), Expect = 7.984e-22
Identity = 49/69 (71.01%), Postives = 55/69 (79.71%), Query Frame = 2

Query:    2 PPHEFLYKEASHCG-PEDGYFACSAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            PPH+FL  +A  CG P       SAV PSLPLTINWLRDCVR NPSL+L+VLVTGSLHLVGDVL+LL+R
Sbjct:  557 PPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 625

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT3G55630.2 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=491)

HSP 1 Score: 60.077 bits (144), Expect = 5.368e-10
Identity = 26/46 (56.52%), Postives = 37/46 (80.43%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            S VF SLP+ ++WLRD  R++  +R QVLVTGSLHLVGD+L+ +++
Sbjct:  446 SFVFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 491

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT3G55630.3 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=492)

HSP 1 Score: 60.077 bits (144), Expect = 5.368e-10
Identity = 26/46 (56.52%), Postives = 37/46 (80.43%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            S VF SLP+ ++WLRD  R++  +R QVLVTGSLHLVGD+L+ +++
Sbjct:  447 SFVFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 492

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT3G55630.1 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=470)

HSP 1 Score: 60.077 bits (144), Expect = 5.368e-10
Identity = 26/46 (56.52%), Postives = 37/46 (80.43%), Query Frame = 2

Query:   68 SAVFPSLPLTINWLRDCVRENPSLRLQVLVTGSLHLVGDVLKLLRR 205
            S VF SLP+ ++WLRD  R++  +R QVLVTGSLHLVGD+L+ +++
Sbjct:  425 SFVFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 470

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT5G05980.2 (| Symbols: ATDFB, DFB, FPGS1 | DHFS-FPGS homolog B | chr5:1799738-1804177 REVERSE LENGTH=513)

HSP 1 Score: 57.7658 bits (138), Expect = 2.664e-9
Identity = 34/56 (60.71%), Postives = 39/56 (69.64%), Query Frame = 2

Query:   41 GPEDGYFACSAVFPSLPLTINWLRDCVRENPSL-RLQVLVTGSLHLVGDVLKLLRR 205
            G  DG    S VF SLP+ I  LRD V E+ S  R QVLVTGSLHLVGDVL+L+R+
Sbjct:  461 GESDGN---SEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIRK 513

[Close]

BLAST of EL585376 vs. TAIR peptide
Match: AT5G05980.1 (| Symbols: ATDFB, DFB, FPGS1 | DHFS-FPGS homolog B | chr5:1799738-1804441 REVERSE LENGTH=571)

HSP 1 Score: 57.7658 bits (138), Expect = 2.664e-9
Identity = 34/56 (60.71%), Postives = 39/56 (69.64%), Query Frame = 2

Query:   41 GPEDGYFACSAVFPSLPLTINWLRDCVRENPSL-RLQVLVTGSLHLVGDVLKLLRR 205
            G  DG    S VF SLP+ I  LRD V E+ S  R QVLVTGSLHLVGDVL+L+R+
Sbjct:  519 GESDGN---SEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIRK 571

The following BLAST results are available for this feature:

BLAST of EL585376 vs. TrEMBL
BLAST of EL585376 vs. TAIR peptide

BLAST of EL585376 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9IHV7_POPTR	1.186e-25	80.88	Folylpolyglutamate synthase OS=Populus trichocarpa...	[more]
B9H9H1_POPTR	2.641e-25	80.88	Folylpolyglutamate synthase OS=Populus trichocarpa...	[more]
D7TII9_VITVI	6.732e-21	75.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6T5X1_SOYBN	1.959e-20	73.53	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q9SR80_ARATH	1.270e-19	71.01	Putative folylpolyglutamate synthetase OS=Arabidop...	[more]
Q8W038_ARATH	1.270e-19	71.01	Folylpolyglutamate synthetase, mitochondrial isofo...	[more]
A2RVP0_ARATH	1.270e-19	71.01	At3g10160 OS=Arabidopsis thaliana PE=2 SV=1	[more]
D7TLM3_VITVI	9.744e-12	73.91	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SE17_RICCO	2.835e-11	71.74	Folylpolyglutamate synthase, putative OS=Ricinus c...	[more]
B9HT54_POPTR	1.838e-10	71.11	Folylpolyglutamate synthase OS=Populus trichocarpa...	[more]

BLAST of EL585376 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G10160.1	7.984e-22	71.01	\| Symbols: ATDFC, DFC, FPGS2 \| DHFS-FPGS homolog C...	[more]
AT3G55630.2	5.368e-10	56.52	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT3G55630.3	5.368e-10	56.52	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT3G55630.1	5.368e-10	56.52	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT5G05980.2	2.664e-9	60.71	\| Symbols: ATDFB, DFB, FPGS1 \| DHFS-FPGS homolog B...	[more]
AT5G05980.1	2.664e-9	60.71	\| Symbols: ATDFB, DFB, FPGS1 \| DHFS-FPGS homolog B...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004101	Mur ligase, C-terminal	GENE3D	3.90.190.20	no description	coord: 6..67 score: 0.0

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EL585376 ID=EL585376; Name=EL585376; organism=Cicer arietinum; type=EST; length=429bp
ACCACCCCATGAATTTCTTTATAAAGAGGCTTCTCATTGTGGTCCAGAAG
ATGGATATTTTGCTTGTAGTGCGGTTTTTCCTTCATTACCATTGACCATA
AATTGGTTGAGGGATTGTGTTAGAGAAAACCCTTCCCTTAGACTTCAGGT
TCTTGTCACTGGCTCATTGCATCTTGTTGGCGATGTATTGAAGCTACTAA
GGAGGTAATGTAAAATTTCCCTCCCCAAGTGGTTGGGGGGTAGTTGATGG
AGACTGGAGAGTGTGATATCGTCGGAACATTTTTCGGAGTTTCTCATGCG
ACGCCAAGGTAGTTGATGGAGAGTGATATCCTTGGAATGTTTTTGAGAGT
TTCTCATGCGACGCAACCCAGTATTCTTTTCCTAGAAATTGAATTTTGCG
CCATTATATTGTCATTGTTATAGTTTCAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0016874	ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009058	biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR004101	Mur_ligase_C

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