GR402742

EST Overview
NameGR402742
Unique NameGR402742
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length303
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
BLAST of GR402742 vs. SwissProt
Match: FAD3E_PHAAU (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Phaseolus aureus GN=ARG1 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.477e-14
Identity = 33/43 (76.74%), Postives = 39/43 (90.70%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP PFHLLKY  QS+SQDHFVSD+GDIV+YQTD KLH+++WTK
Sbjct:  336 GPLPFHLLKYLLQSISQDHFVSDTGDIVYYQTDPKLHQDSWTK 378          
BLAST of GR402742 vs. SwissProt
Match: FAD3E_SOYBN (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max GN=FAD3 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.435e-9
Identity = 27/37 (72.97%), Postives = 31/37 (83.78%), Query Frame = -1
Query:  193 PFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTD-LKLH 300
            P PFHL+KY  QSM QDHFVSD+GD+V+YQTD L LH
Sbjct:  340 PLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLLLH 376          
BLAST of GR402742 vs. SwissProt
Match: FAD3E_TOBAC (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.601e-8
Identity = 24/33 (72.73%), Postives = 28/33 (84.85%), Query Frame = -1
Query:  205 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTD 303
            GP PFHL+K   +SM QDH+VSDSG+IVFYQTD
Sbjct:  336 GPIPFHLVKDLTRSMKQDHYVSDSGEIVFYQTD 368          
BLAST of GR402742 vs. TrEMBL
Match: Q9FXK9_SOYBN (Microsomal omega-3 fatty acid desaturase OS=Glycine max GN=FAD3C PE=2 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 3.734e-11
Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSDSGDIV+YQTD +LH+++WT+
Sbjct:  336 GPLPLHLIKYLLHSISQDHFVSDSGDIVYYQTDSQLHKDSWTQ 378          
BLAST of GR402742 vs. TrEMBL
Match: B5LSX5_SOYBN (Microsomal omega-3 fatty acid desaturase OS=Glycine max GN=FAD3 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.734e-11
Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSDSGDIV+YQTD +LH+++WT+
Sbjct:  336 GPLPLHLIKYLLHSISQDHFVSDSGDIVYYQTDSQLHKDSWTQ 378          
BLAST of GR402742 vs. TrEMBL
Match: B3GCK6_SOYBN (Omega-3 fatty acid desaturase OS=Glycine max GN=FAD3C PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.734e-11
Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSDSGDIV+YQTD +LH+++WT+
Sbjct:  336 GPLPLHLIKYLLHSISQDHFVSDSGDIVYYQTDSQLHKDSWTQ 378          
BLAST of GR402742 vs. TrEMBL
Match: C6TKV5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.086e-10
Identity = 28/43 (65.12%), Postives = 35/43 (81.40%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSD GDIV+YQTD + H+++WTK
Sbjct:  337 GPLPLHLIKYLLHSISQDHFVSDYGDIVYYQTDFQFHKDSWTK 379          
BLAST of GR402742 vs. TrEMBL
Match: Q68Y59_SOYBN (Microsomal omega-3 fatty acid desaturase OS=Glycine max GN=GmFAD3-2b PE=2 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 1.853e-10
Identity = 28/43 (65.12%), Postives = 35/43 (81.40%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSD GDIV+YQTD + H+++WTK
Sbjct:  337 GPLPLHLIKYLLHSISQDHFVSDYGDIVYYQTDSQFHKDSWTK 379          
BLAST of GR402742 vs. TrEMBL
Match: B3GCK5_SOYBN (Omega-3 fatty acid desaturase OS=Glycine max GN=FAD3C PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.853e-10
Identity = 28/43 (65.12%), Postives = 35/43 (81.40%), Query Frame = -1
Query:  175 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHENTWTK 303
            GP P HL+KY   S+SQDHFVSD GDIV+YQTD + H+++WTK
Sbjct:  336 GPLPLHLIKYLLHSISQDHFVSDYGDIVYYQTDSQFHKDSWTK 378          
BLAST of GR402742 vs. TrEMBL
Match: B6CI31_VIGUN (Microsomal omega-3 fatty acid desaturase OS=Vigna unguiculata GN=FAD3 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.017e-8
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = -1
Query:  187 PFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLHEN 300
            P PFHL+KY  QSM +DHFVSD+GD+V+YQTD  LH++
Sbjct:  334 PIPFHLIKYLMQSMREDHFVSDTGDVVYYQTDPHLHQD 371          
BLAST of GR402742 vs. TrEMBL
Match: Q84X84_SOYBN (Microsomal omega-3-fatty acid desaturase OS=Glycine max GN=FAD3A PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.590e-8
Identity = 27/39 (69.23%), Postives = 32/39 (82.05%), Query Frame = -1
Query:  187 PFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTD-LKLHEN 300
            P PFHL+KY  QSM QDHFVSD+GD+V+YQTD L LH +
Sbjct:  336 PLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLHLHSH 374          
BLAST of GR402742 vs. TrEMBL
Match: B3F7U7_SOYBN (Microsomal omega-3 fatty acid desaturase A OS=Glycine max GN=FAD3A PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.590e-8
Identity = 27/39 (69.23%), Postives = 32/39 (82.05%), Query Frame = -1
Query:  187 PFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTD-LKLHEN 300
            P PFHL+KY  QSM QDHFVSD+GD+V+YQTD L LH +
Sbjct:  336 PLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLHLHSH 374          
BLAST of GR402742 vs. TrEMBL
Match: A4UHR9_SOYBN (Microsomal omega three fatty acid desaturase OS=Glycine max GN=FAD3A PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.607e-8
Identity = 27/37 (72.97%), Postives = 31/37 (83.78%), Query Frame = -1
Query:  193 PFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTD-LKLH 300
            P PFHL+KY  QSM QDHFVSD+GD+V+YQTD L LH
Sbjct:  336 PLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLHLH 372          
BLAST of GR402742 vs. TAIR peptide
Match: AT5G05580.1 (| Symbols: FAD8 | fatty acid desaturase 8 | chr5:1664331-1666345 FORWARD LENGTH=435)

HSP 1 Score: 51.9878 bits (123), Expect = 8.935e-8
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = -1
Query:  193 GPFPFHLLKYFQQSMSQDHFVSDSGDIVFYQTDLKLH 303
            GP P HLL    +SM QDHFVSD+GD+V+Y+ D KL+
Sbjct:  395 GPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKLN 431          
The following BLAST results are available for this feature:
BLAST of GR402742 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
FAD3E_PHAAU1.477e-1476.74Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3E_SOYBN5.435e-972.97Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3E_TOBAC4.601e-872.73Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
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BLAST of GR402742 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9FXK9_SOYBN3.734e-1167.44Microsomal omega-3 fatty acid desaturase OS=Glycin... [more]
B5LSX5_SOYBN3.734e-1167.44Microsomal omega-3 fatty acid desaturase OS=Glycin... [more]
B3GCK6_SOYBN3.734e-1167.44Omega-3 fatty acid desaturase OS=Glycine max GN=FA... [more]
C6TKV5_SOYBN1.086e-1065.12Putative uncharacterized protein OS=Glycine max PE... [more]
Q68Y59_SOYBN1.853e-1065.12Microsomal omega-3 fatty acid desaturase OS=Glycin... [more]
B3GCK5_SOYBN1.853e-1065.12Omega-3 fatty acid desaturase OS=Glycine max GN=FA... [more]
B6CI31_VIGUN1.017e-865.79Microsomal omega-3 fatty acid desaturase OS=Vigna ... [more]
Q84X84_SOYBN6.590e-869.23Microsomal omega-3-fatty acid desaturase OS=Glycin... [more]
B3F7U7_SOYBN6.590e-869.23Microsomal omega-3 fatty acid desaturase A OS=Glyc... [more]
A4UHR9_SOYBN8.607e-872.97Microsomal omega three fatty acid desaturase OS=Gl... [more]
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BLAST of GR402742 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G05580.18.935e-859.46| Symbols: FAD8 | fatty acid desaturase 8 | chr5:1... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 42..53
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR402742 ID=GR402742; Name=GR402742; organism=Cicer arietinum; type=EST; length=303bp
GCTTCAAAAATAATGGATTATTGAAAAATAAGTGCATTTCTAACATGCAT
AATAAACAAAATCTTATAGATATCAATCACCTAATTCATTCGGTGAGCTA
TGGTAAAACATTGTAAGTAAGTTAAAAAATATTTGAACGGCACAAATAAA
TTGAGCACAAAAATGTTACTCAAACTTGGTCCAAGTATTTTCGTGGAGCT
TAAGATCTGTTTGGTAAAACACGATGTCTCCGGAATCGCTTACGAAGTGG
TCCTGGCTCATGCTTTGCTGGAAGTACTTTAATAAATGGAATGGGAACGG
CCC
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