GR406364

EST Overview


Name	GR406364
Unique Name	GR406364
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	572

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_ARATH (3-isopropylmalate dehydratase OS=Arabidopsis thaliana GN=IIL1 PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 3.888e-52
Identity = 101/161 (62.73%), Postives = 119/161 (73.91%), Query Frame = 2

Query:   89 MASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MAS + + P      +K DL +S+F         R QK +  +   SV APQ+  R P TTGSVKT MTMTEKILARAS+K  V PGDNIWVNVD+LMTHDVCGPG+ GIFK EFG+ AKVWD EK+V+IPDHYIFT+D+RANRNVDI+R+ C EQNIKYF
Sbjct:    1 MASVISSSPFLCKSSSKSDLGISSFPKSSQISIHRCQKKSISRKIVSVMAPQK-DRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYF 160

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_METJA (Isopropylmalate/citramalate isomerase large subunit OS=Methanocaldococcus jannaschii GN=leuC PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.182e-16
Identity = 43/94 (45.74%), Postives = 63/94 (67.02%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MT+ EKILA+AS K++V+PGD +  N+D+ M HD+ GP ++   K E+G + KVW+ EK+VI+ DH +     +A  N  ++R F  EQ IKYF
Sbjct:    3 MTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLK-EYGIE-KVWNPEKIVILFDHQVPADSIKAAENHILMRKFVKEQGIKYF 94

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC1_METTH (3-isopropylmalate dehydratase large subunit 1 OS=Methanobacterium thermoautotrophicum GN=leuC1 PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.706e-15
Identity = 43/95 (45.26%), Postives = 64/95 (67.37%), Query Frame = 2

Query:  287 AMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            +MT++EKILARAS K++V  G+ +  ++D+ MTHD+ GP S+  F+   G+D +VWD EK+V+I DH +      A +N  I+RDF  EQ I+ F
Sbjct:    2 SMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFR-AIGED-RVWDPEKIVVIFDHQVPADSIEAAQNHMIMRDFVEEQGIRNF 94

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC1_ARCFU (3-isopropylmalate dehydratase large subunit 1 OS=Archaeoglobus fulgidus GN=leuC1 PE=3 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 3.557e-13
Identity = 38/92 (41.30%), Postives = 53/92 (57.61%), Query Frame = 2

Query:  287 AMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNI 562
            A T+ EKI ++AS KE V  G+ +  N+D+ M HD+  P +I  F+   G DAKVWD+ KV++  DH +      A  N  +LR F  EQ I
Sbjct:    2 AQTLVEKIFSKASGKE-VKAGEFVMANIDLAMIHDITAPLAIKAFREILGSDAKVWDKSKVIMAFDHQVPADSVHAAENHKMLRKFAEEQGI 92

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_SYNAS (3-isopropylmalate dehydratase large subunit OS=Syntrophus aciditrophicus (strain SB) GN=leuC PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.068e-13
Identity = 41/94 (43.62%), Postives = 57/94 (60.64%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MT+TEKIL R +  ++V PG  I   VDI + +DV  P +I  F+   GK  KV+DR+KVV+IPDH+    D  +     I+RDF  EQ I ++
Sbjct:    3 MTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGK--KVFDRDKVVLIPDHFTPNKDINSAAQCKIMRDFAREQEITHY 94

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_DESAA (3-isopropylmalate dehydratase large subunit OS=Desulfatibacillum alkenivorans (strain AK-01) GN=leuC PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.352e-12
Identity = 36/94 (38.30%), Postives = 60/94 (63.83%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MT+ EKILA  + +E+ +PGD I   VDI + +D+  P +I +F+      AKV+D+++VV++PDH+  T D  +   V ++RDF  EQ + ++
Sbjct:    3 MTIAEKILAAHAGQEEASPGDLINAKVDIALGNDITAPIAIKLFRQSGA--AKVFDKDRVVLVPDHFTPTKDINSAMQVKMVRDFAREQELSHW 94

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_THEYD (3-isopropylmalate dehydratase large subunit OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) GN=leuC PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.144e-11
Identity = 36/94 (38.30%), Postives = 59/94 (62.77%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MT+ EKILA  + K++VTPG+ I   VDI++ +D+  P +I  F+    KD  V+DRE++ ++PDH+    D +A     +LR+F  +  IK++
Sbjct:    3 MTIAEKILAAHAGKKEVTPGEIINAKVDIVLANDITAPIAIKEFEKLGVKD--VFDRERIALVPDHFTPQKDIKAAEQCKMLREFAQKYKIKHY 94

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_CALS8 (3-isopropylmalate dehydratase large subunit OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=leuC PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.549e-11
Identity = 38/95 (40.00%), Postives = 63/95 (66.32%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNI-KYF 571
            MTM++KILA  + KE V PGD I+ NVD+++ +DV  P +I  F+ + G D KV+D++K+ I+PDH+    D ++ +   ++R+F  + +I  YF
Sbjct:    5 MTMSQKILAYHAGKEYVEPGDLIFANVDLVLGNDVTTPVAIKEFE-KIGVD-KVFDKDKITIVPDHFTPNKDIKSAQQCKMVREFAKKYDITNYF 97

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC2_METTH (3-isopropylmalate dehydratase large subunit 2 OS=Methanobacterium thermoautotrophicum GN=leuC2 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.330e-11
Identity = 38/93 (40.86%), Postives = 54/93 (58.06%), Query Frame = 2

Query:  290 MTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKN--EFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNI 562
            M MTEKILA A+   +VTPG+ I   VD+ MTHD   P +I  F++    G  A+VWD E++V++ DH +  +   A     + R+F  EQ I
Sbjct:    4 MNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGAAEFQRVTREFAREQGI 96

[Close]

BLAST of GR406364 vs. SwissProt
Match: LEUC_DESHY (3-isopropylmalate dehydratase large subunit OS=Desulfitobacterium hafniense (strain Y51) GN=leuC PE=3 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 5.680e-11
Identity = 36/95 (37.89%), Postives = 57/95 (60.00%), Query Frame = 2

Query:  287 AMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            AMT+TEKILA  +  ++V PG  I   +D+ + +D+ GP SI  F+ +FG +  VWD+ KV ++PDH+    D  +      LR+F  +Q I ++
Sbjct:    2 AMTITEKILAEHAGLDKVVPGQLITAQLDLALANDITGPVSIREFE-KFGVET-VWDKTKVALVPDHFTPNKDIASAELSKALREFAKKQGIVHY 94

[Close]

BLAST of GR406364 vs. TrEMBL
Match: B9SQS5_RICCO (3-isopropylmalate dehydratase, putative OS=Ricinus communis GN=RCOM_1217310 PE=4 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 5.354e-59
Identity = 115/163 (70.55%), Postives = 135/163 (82.82%), Query Frame = 2

Query:   89 MASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQ--RYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS +  PS+SF  TKKDL LSAF           + +K  S+K+  SV APQQ++R P+TTGSVKTAMTMTEKILARAS+K Q++PG+N+WVNVD+LMTHDVCGPGSIGIFK EFG++A VWDREK+VIIPDHYIFT+DERANRNVDILRDFC EQN+KYF
Sbjct:    1 MASSAITSPSSSFI-TKKDLGLSAFSSQSSPFYSIPKCKKSISKKVV-SVMAPQQSERTPATTGSVKTAMTMTEKILARASEKSQLSPGENVWVNVDVLMTHDVCGPGSIGIFKREFGQNATVWDREKIVIIPDHYIFTTDERANRNVDILRDFCQEQNVKYF 161

[Close]

BLAST of GR406364 vs. TrEMBL
Match: D7TL65_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_56.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029978001 PE=4 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 1.319e-57
Identity = 113/161 (70.19%), Postives = 129/161 (80.12%), Query Frame = 2

Query:   89 MASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS ++  ST+F   KKD+ LSAF            K  + +   SV APQQ+ R PSTTGSVKTAMTMTEKILARASQK Q++PG+N+WV+VDILMTHDVCGPGS GIFK EFG++AKVWDREK+VIIPDHYIFT+DERANRNVDILRDFC EQNIKYF
Sbjct:    1 MASSTISSNSTTFI--KKDVGLSAFSSSPSSFSVPKTKRRNSRKIVSVMAPQQSPRSPSTTGSVKTAMTMTEKILARASQKPQLSPGENVWVDVDILMTHDVCGPGSFGIFKKEFGQNAKVWDREKIVIIPDHYIFTNDERANRNVDILRDFCTEQNIKYF 159

[Close]

BLAST of GR406364 vs. TrEMBL
Match: D7SZP6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_49.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027922001 PE=4 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.116e-56
Identity = 114/162 (70.37%), Postives = 132/162 (81.48%), Query Frame = 2

Query:   89 MASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXX-QRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS +A  S +F   KKD++LSAF         Q+  K  SRKI  SV APQQ+ R PST+GSVKTAMTMTEKILARAS+K Q++PG+N+WVNVD+LMTHDVCGPGS GIFK EFG++AKVWDREK+VIIP+HYIFT DERANRNVDILR+FC EQNIKYF
Sbjct:    1 MASSAIASNSPAFI--KKDVDLSAFSSSASSFSIQKTNKRNSRKIV-SVMAPQQSPRSPSTSGSVKTAMTMTEKILARASEKPQLSPGENVWVNVDLLMTHDVCGPGSFGIFKKEFGENAKVWDREKIVIIPEHYIFTKDERANRNVDILREFCTEQNIKYF 159

[Close]

BLAST of GR406364 vs. TrEMBL
Match: B9HSU8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_821804 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 4.390e-53
Identity = 97/112 (86.61%), Postives = 107/112 (95.54%), Query Frame = 2

Query:  236 APQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            APQQ++R+P+TTGSVKTA+TMTEKI ARAS+K Q+ PG+N+WVNVDILMTHDVCGPGSIGIFK EFG+DAKVWDREKVVIIPDHYIFT DERANRNVDILRDFCNEQNIKYF
Sbjct:    2 APQQSERRPATTGSVKTALTMTEKIFARASEKPQLIPGENVWVNVDILMTHDVCGPGSIGIFKKEFGQDAKVWDREKVVIIPDHYIFTKDERANRNVDILRDFCNEQNIKYF 113

[Close]

BLAST of GR406364 vs. TrEMBL
Match: C4JBG7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.479e-50
Identity = 105/163 (64.42%), Postives = 126/163 (77.30%), Query Frame = 2

Query:   89 MASSL--VAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS+   A  S +F H K+   L+A         +R +    R    +V++P +T R PS+TGSVKTAMTMTEKILARAS++  + PG+N+WV++D+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFC EQNIKYF
Sbjct:    1 MASSISAAAKASAAFAHKKE---LAAPAPHRAGSSRRTKPCRVR----AVASPARTPRAPSSTGSVKTAMTMTEKILARASERAALEPGENVWVDIDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCLEQNIKYF 156

[Close]

BLAST of GR406364 vs. TrEMBL
Match: C4IZQ5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.479e-50
Identity = 105/163 (64.42%), Postives = 126/163 (77.30%), Query Frame = 2

Query:   89 MASSL--VAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS+   A  S +F H K+   L+A         +R +    R    +V++P +T R PS+TGSVKTAMTMTEKILARAS++  + PG+N+WV++D+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFC EQNIKYF
Sbjct:    1 MASSISAAAKASAAFAHKKE---LAAPAPHRAGSSRRTKPCRVR----AVASPARTPRAPSSTGSVKTAMTMTEKILARASERAALEPGENVWVDIDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCLEQNIKYF 156

[Close]

BLAST of GR406364 vs. TrEMBL
Match: B6TYJ6_MAIZE (3-isopropylmalate dehydratase large subunit 2 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.479e-50
Identity = 105/164 (64.02%), Postives = 125/164 (76.22%), Query Frame = 2

Query:   89 MASSL-VAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICC--SVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS+  A  +++ F  KK+L   A          R       K C   +V++P +T R PS+TGSVKTAMTMTEKILARAS++  + PG+N+WV++D+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFC EQNIKYF
Sbjct:    1 MASSISAAAKASAAFAQKKELAAPA--------PHRAGSSRRTKPCRVRAVASPARTPRAPSSTGSVKTAMTMTEKILARASERAALEPGENVWVDIDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCLEQNIKYF 156

[Close]

BLAST of GR406364 vs. TrEMBL
Match: Q6Z702_ORYSJ (Os02g0125100 protein OS=Oryza sativa subsp. japonica GN=P0575F10.36 PE=2 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 5.933e-50
Identity = 102/161 (63.35%), Postives = 123/161 (76.40%), Query Frame = 2

Query:   92 ASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRK-ICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            A+S VA  + +F H K +L  +A         +R     +R     +V+ P +  R PS+TGSVK+AMTMTEKILARAS++  + PG+N+WV+VD+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFC EQNIKYF
Sbjct:    6 AASPVAGKAAAFAH-KNELAAAAVAPSQQQLQRRVSGRRARSGRVRAVATPARAPRAPSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYF 165

[Close]

BLAST of GR406364 vs. TrEMBL
Match: C5XT62_SORBI (Putative uncharacterized protein Sb04g002120 OS=Sorghum bicolor GN=Sb04g002120 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 5.933e-50
Identity = 105/163 (64.42%), Postives = 126/163 (77.30%), Query Frame = 2

Query:   89 MASSL--VAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MASS+   A  ST+F H KK+L   A         +R +    R    +V++P +  R PS+TGSVK+AMTMTEKILARAS++  + PG+N+WV+VD+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFT+DERANRNVDILRDFC EQNIKYF
Sbjct:    1 MASSISAAAKASTAFAH-KKELAAPAPAQHRAGSSRRTKPCRVR----AVASPARAPRAPSSTGSVKSAMTMTEKILARASERAALEPGENVWVDVDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTTDERANRNVDILRDFCLEQNIKYF 158

[Close]

BLAST of GR406364 vs. TrEMBL
Match: B8AGY8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_05669 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 5.933e-50
Identity = 102/161 (63.35%), Postives = 123/161 (76.40%), Query Frame = 2

Query:   92 ASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRK-ICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            A+S VA  + +F H K +L  +A         +R     +R     +V+ P +  R PS+TGSVK+AMTMTEKILARAS++  + PG+N+WV+VD+LMTHDVCGPG+IGIFK EFG+DAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFC EQNIKYF
Sbjct:    6 AASPVAGKAAAFAH-KNELAAAAVAPSQQQLQRRVSGRRARSGRVRAVATPARAPRAPSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYF 165

[Close]

BLAST of GR406364 vs. TAIR peptide
Match: AT4G13430.1 (| Symbols: IIL1, ATLEUC1 | isopropyl malate isomerase large subunit 1 | chr4:7804194-7807789 REVERSE LENGTH=509)

HSP 1 Score: 204.142 bits (518), Expect = 4.139e-53
Identity = 101/161 (62.73%), Postives = 119/161 (73.91%), Query Frame = 2

Query:   89 MASSLVAPPSTSFFHTKKDLNLSAFXXXXXXXXQRYQKHASRKICCSVSAPQQTQRQPSTTGSVKTAMTMTEKILARASQKEQVTPGDNIWVNVDILMTHDVCGPGSIGIFKNEFGKDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCNEQNIKYF 571
            MAS + + P      +K DL +S+F         R QK +  +   SV APQ+  R P TTGSVKT MTMTEKILARAS+K  V PGDNIWVNVD+LMTHDVCGPG+ GIFK EFG+ AKVWD EK+V+IPDHYIFT+D+RANRNVDI+R+ C EQNIKYF
Sbjct:    1 MASVISSSPFLCKSSSKSDLGISSFPKSSQISIHRCQKKSISRKIVSVMAPQK-DRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYF 160

The following BLAST results are available for this feature:

BLAST of GR406364 vs. SwissProt
BLAST of GR406364 vs. TrEMBL
BLAST of GR406364 vs. TAIR peptide

BLAST of GR406364 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LEUC_ARATH	3.888e-52	62.73	3-isopropylmalate dehydratase OS=Arabidopsis thali...	[more]
LEUC_METJA	1.182e-16	45.74	Isopropylmalate/citramalate isomerase large subuni...	[more]
LEUC1_METTH	1.706e-15	45.26	3-isopropylmalate dehydratase large subunit 1 OS=M...	[more]
LEUC1_ARCFU	3.557e-13	41.30	3-isopropylmalate dehydratase large subunit 1 OS=A...	[more]
LEUC_SYNAS	6.068e-13	43.62	3-isopropylmalate dehydratase large subunit OS=Syn...	[more]
LEUC_DESAA	1.352e-12	38.30	3-isopropylmalate dehydratase large subunit OS=Des...	[more]
LEUC_THEYD	1.144e-11	38.30	3-isopropylmalate dehydratase large subunit OS=The...	[more]
LEUC_CALS8	2.549e-11	40.00	3-isopropylmalate dehydratase large subunit OS=Cal...	[more]
LEUC2_METTH	3.330e-11	40.86	3-isopropylmalate dehydratase large subunit 2 OS=M...	[more]
LEUC_DESHY	5.680e-11	37.89	3-isopropylmalate dehydratase large subunit OS=Des...	[more]

BLAST of GR406364 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SQS5_RICCO	5.354e-59	70.55	3-isopropylmalate dehydratase, putative OS=Ricinus...	[more]
D7TL65_VITVI	1.319e-57	70.19	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SZP6_VITVI	1.116e-56	70.37	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HSU8_POPTR	4.390e-53	86.61	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C4JBG7_MAIZE	3.479e-50	64.42	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C4IZQ5_MAIZE	3.479e-50	64.42	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TYJ6_MAIZE	3.479e-50	64.02	3-isopropylmalate dehydratase large subunit 2 OS=Z...	[more]
Q6Z702_ORYSJ	5.933e-50	63.35	Os02g0125100 protein OS=Oryza sativa subsp. japoni...	[more]
C5XT62_SORBI	5.933e-50	64.42	Putative uncharacterized protein Sb04g002120 OS=So...	[more]
B8AGY8_ORYSI	5.933e-50	63.35	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GR406364 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G13430.1	4.139e-53	62.73	\| Symbols: IIL1, ATLEUC1 \| isopropyl malate isomer...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	SUPERFAMILY	53732	Aconitase iron-sulfur domain	coord: 88..190 score: 3.5
IPR015931	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	GENE3D	3.30.499.10	no description	coord: 93..189 score: 6.1
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit	PANTHER	PTHR11670:SF6	3-ISOPROPYLMALATE DEHYDRATASE	coord: 63..190 score: 3.6
IPR015937	Aconitase/isopropylmalate dehydratase	PANTHER	PTHR11670	ACONITASE	coord: 63..190 score: 3.6
None	No IPR available	SEG	seg	seg	coord: 55..62 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR406364 ID=GR406364; Name=GR406364; organism=Cicer arietinum; type=EST; length=572bp
ACAACTACTCTTACTACCTACCTATTTTCATTTCTTCAGCCGCCACACAT
TGTTGTGTAGTGTTGTTGTTGTTATGCCTCAAACATCAATGGCTTCTTCT
CTTGTTGCTCCACCCTCCACTTCCTTCTTCCACACCAAGAAGGATCTGAA
TCTATCTGCTTTCTCTTCATCTTCGTCTTCTTCATCTCAGCGATATCAGA
AACATGCTTCCAGGAAAATCTGTTGTTCGGTTTCGGCGCCTCAGCAAACA
CAGCGCCAGCCTTCTACCACTGGATCAGTGAAGACAGCTATGACTATGAC
CGAGAAGATATTGGCTCGAGCTTCTCAGAAGGAACAGGTTACACCTGGGG
ATAATATTTGGGTCAATGTTGATATTTTGATGACTCATGATGTTTGTGGC
CCTGGTTCTATTGGGATTTTTAAGAACGAGTTTGGTAAGGATGCTAAGGT
TTGGGATCGTGAAAAGGTTGTAATAATACCTGATCACTATATCTTCACAA
GTGATGAACGTGCCAATCGTAATGTAGACATACTCAGAGATTTCTGCAAT
GAGCAAAATATCAAGTACTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0008652	amino acid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016836	hydro-lyase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001030	Acoase/IPM_deHydtase_lsu_aba
IPR015931	Acnase/IPM_dHydase_lsu_aba_1/3
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit
IPR015937	Acoase/IPM_deHydtase

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