GR420463

EST Overview


Name	GR420463
Unique Name	GR420463
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	520

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR420463 vs. TrEMBL
Match: B2BAK4_LILLO (Cytochrome P450-like TBP protein (Fragment) OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.403e-23
Identity = 34/41 (82.93%), Postives = 35/41 (85.37%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVFRPD P    LGSK+RG APPP
Sbjct:   28 LRYLLTDVPPQPNSPPDNVFRPDRPAGAGLGSKRRGKAPPP 68

HSP 2 Score: 41.9726 bits (97), Expect = 5.403e-23
Identity = 28/53 (52.83%), Postives = 29/53 (54.72%), Query Frame = 2

Query:  206 PIHGISKITLK----------GSHFKSFHKVILHLRTRVKLNCGFAGRVFFPR 334
            P HGISKITLK          GSH     +VI   RTRVKLN     RVFFPR
Sbjct:   68 PHHGISKITLKVVVFHLRRQAGSHLSYTSQVISQSRTRVKLN-----RVFFPR 115

HSP 3 Score: 32.7278 bits (73), Expect = 5.403e-23
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:    3 PHEISVLVELILGHFTLL 56
            PHEISVLVELILGH   L
Sbjct:   14 PHEISVLVELILGHLRYL 31

[Close]

BLAST of GR420463 vs. TrEMBL
Match: Q0WLP3_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 6.387e-19
Identity = 33/41 (80.49%), Postives = 34/41 (82.93%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNV RPD P E SLG+K RG  PPP
Sbjct:   82 LRYLLTDVPPQPNSPPDNVLRPDRPAEASLGTKNRGCTPPP 122

HSP 2 Score: 42.743 bits (99), Expect = 6.387e-19
Identity = 27/51 (52.94%), Postives = 29/51 (56.86%), Query Frame = 2

Query:  206 PIHGISKITLK--------GSHFKSFHKVILHLRTRVKLNCGFAGRVFFPR 334
            PIHG+SKITLK         SH     +VI   RTRVKLN     RVFFPR
Sbjct:  122 PIHGVSKITLKVVVFHLRRSSHLSYTSQVISQSRTRVKLN-----RVFFPR 167

[Close]

BLAST of GR420463 vs. TrEMBL
Match: B2KZF9_PICAB (Senescence-associated protein OS=Picea abies GN=SAP1 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.688e-16
Identity = 32/41 (78.05%), Postives = 34/41 (82.93%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVFRPD P E  L +K RG+AP P
Sbjct:   82 LRYLLTDVPPQPNSPPDNVFRPDRPVETCLKAKNRGNAPSP 122

HSP 2 Score: 32.7278 bits (73), Expect = 1.688e-16
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:    3 PHEISVLVELILGHFTLL 56
            PHEISVLVELILGH   L
Sbjct:   68 PHEISVLVELILGHLRYL 85

HSP 3 Score: 25.0238 bits (53), Expect = 1.688e-16
Identity = 16/28 (57.14%), Postives = 17/28 (60.71%), Query Frame = 2

Query:  206 PIHGISKITLKGSHFKSFHK---VILHL 280
            P HGISKITLK   F    K   +ILHL
Sbjct:  122 PPHGISKITLKVVVFHLRRKRLPLILHL 149

[Close]

BLAST of GR420463 vs. TrEMBL
Match: Q9AVH2_PEA (Putative senescence-associated protein (Fragment) OS=Pisum sativum GN=ssa-13 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.628e-16
Identity = 33/41 (80.49%), Postives = 34/41 (82.93%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVFRPD PT+VSL  KK G   PP
Sbjct:  172 LRYLLTDVPPQPNSPPDNVFRPDRPTKVSLWIKKEGQRRPP 212

HSP 2 Score: 37.3502 bits (85), Expect = 2.628e-16
Identity = 27/56 (48.21%), Postives = 30/56 (53.57%), Query Frame = 3

Query:  240 APTSSHFTKLSYTSG-LEXXXXXXXXXXXXFPADSAKPVPL-----DSRQGQWESR 389
            A +SSH   LSYTS  +             FPADSAK VPL     DSR+GQWE R
Sbjct:  230 AVSSSH---LSYTSQVISQSRTRVKLNRVFFPADSAKAVPLLLVSLDSRKGQWEFR 282

[Close]

BLAST of GR420463 vs. TrEMBL
Match: B7FKH8_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.722e-15
Identity = 38/41 (92.68%), Postives = 39/41 (95.12%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVFRPD PT+V LGSKKRGSAPPP
Sbjct:   82 LRYLLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPP 122

[Close]

BLAST of GR420463 vs. TrEMBL
Match: A9TVA3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_151305 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.947e-11
Identity = 27/42 (64.29%), Postives = 29/42 (69.05%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTE-VSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVF  D  T    L ++ R   PPP
Sbjct:   67 LRYLLTDVPPQPNSPPDNVFHLDRRTRGARLKTRSRDRVPPP 108

HSP 2 Score: 32.7278 bits (73), Expect = 1.947e-11
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:    3 PHEISVLVELILGHFTLL 56
            PHEISVLVELILGH   L
Sbjct:   53 PHEISVLVELILGHLRYL 70

HSP 3 Score: 24.6386 bits (52), Expect = 1.947e-11
Identity = 14/26 (53.85%), Postives = 15/26 (57.69%), Query Frame = 2

Query:  206 PIHGISKITLKGSHFKSFHKVILHLR 283
            P HGISKITLK         V+ HLR
Sbjct:  108 PPHGISKITLK--------VVVFHLR 125

[Close]

BLAST of GR420463 vs. TrEMBL
Match: A9U6L8_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_157767 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.794e-10
Identity = 27/42 (64.29%), Postives = 29/42 (69.05%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTE-VSLGSKKRGSAPPP 180
            LRYLLTDVPPQPNSPPDNVF  D  T    L ++ R   PPP
Sbjct:   67 LRYLLTDVPPQPNSPPDNVFHLDRRTRGARLKTRSRDRVPPP 108

HSP 2 Score: 32.7278 bits (73), Expect = 6.794e-10
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:    3 PHEISVLVELILGHFTLL 56
            PHEISVLVELILGH   L
Sbjct:   53 PHEISVLVELILGHLRYL 70

[Close]

BLAST of GR420463 vs. TrEMBL
Match: C1NAM9_MICPS (Senescence-associated protein OS=Micromonas pusilla CCMP1545 GN=SSA13 PE=4 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.389e-9
Identity = 24/33 (72.73%), Postives = 26/33 (78.79%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSK 156
            LRYLLTDVPPQPNSPPDNV RPD   + + G K
Sbjct:   67 LRYLLTDVPPQPNSPPDNVLRPDQLPKQAFGPK 99

HSP 2 Score: 32.7278 bits (73), Expect = 1.389e-9
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:    3 PHEISVLVELILGHFTLL 56
            PHEISVLVELILGH   L
Sbjct:   53 PHEISVLVELILGHLRYL 70

[Close]

BLAST of GR420463 vs. TrEMBL
Match: O04892_TOBAC (Cytochrome P450 like_TBP OS=Nicotiana tabacum GN=cTBP PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.738e-9
Identity = 42/77 (54.55%), Postives = 43/77 (55.84%), Query Frame = 2

Query:   77 MCRPSQTPHLTMSSARIGQPKSALDPKRGAAPRLRSGISLSLFPIHGISKITLKGSHF-------------KSFHKV 268
            MCRPSQTPHLTMSSARIG   S    KRG+AP           PIHGISKITLK   F             KSFHKV
Sbjct:    1 MCRPSQTPHLTMSSARIGLQASLGSKKRGSAP----------LPIHGISKITLKVVVFHFRLSAPTYTTPLKSFHKV 67

[Close]

BLAST of GR420463 vs. TrEMBL
Match: C4J720_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 8.625e-9
Identity = 29/39 (74.36%), Postives = 30/39 (76.92%), Query Frame = 1

Query:   58 LRYLLTDVPPQPNSPPDNVFRPDWPTEVSLGSKKRGSAP 174
            LRYLLTDVPPQPNSPPDNVFRPD P E    S+K G  P
Sbjct:   82 LRYLLTDVPPQPNSPPDNVFRPDRPGEAGPWSQKEGRCP 120

The following BLAST results are available for this feature:

BLAST of GR420463 vs. TrEMBL

BLAST of GR420463 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B2BAK4_LILLO	5.403e-23	82.93	Cytochrome P450-like TBP protein (Fragment) OS=Lil...	[more]
Q0WLP3_ARATH	6.387e-19	80.49	Putative uncharacterized protein OS=Arabidopsis th...	[more]
B2KZF9_PICAB	1.688e-16	78.05	Senescence-associated protein OS=Picea abies GN=SA...	[more]
Q9AVH2_PEA	2.628e-16	80.49	Putative senescence-associated protein (Fragment) ...	[more]
B7FKH8_MEDTR	4.722e-15	92.68	Putative uncharacterized protein OS=Medicago trunc...	[more]
A9TVA3_PHYPA	1.947e-11	64.29	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9U6L8_PHYPA	6.794e-10	64.29	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
C1NAM9_MICPS	1.389e-9	72.73	Senescence-associated protein OS=Micromonas pusill...	[more]
O04892_TOBAC	1.738e-9	54.55	Cytochrome P450 like_TBP OS=Nicotiana tabacum GN=c...	[more]
C4J720_MAIZE	8.625e-9	74.36	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 19..30 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420463 ID=GR420463; Name=GR420463; organism=Cicer arietinum; type=EST; length=520bp
TTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACATTTTACC
CTTTTGCCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCC
CACCTGACAATGTCTTCCGCCCGGATTGGCCAACCGAAGTCAGCCTTGGA
TCCAAAAAGAGGGGCAGCGCCCCGCCTCCGTAGTGGTATTTCACTTTCGC
TGTTTCCAATTCACGGAATAAGTAAAATAACGTTAAAAGGCTCCCACTTC
AAGTCATTTCACAAAGTTATCCTACACCTCCGGACTAGAGTCAAGCTCAA
CTGTGGTTTCGCTGGCAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCC
GTTCCCTTGGATAGTAGACAGGGACAGTGGGAATCTCGGTCACTAATTAG
ATTTAATCCATTCATGCGCGTTGGCTACCTTAAGAACGAGGCATGAGTCA
TAGTTACTCCCGCCGTTTACCCGCGCTTGGAGCACTGGGCAGAATTGAAT
TTCTTCACTTTGACATTCAG

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