GR915536

EST Overview


Name	GR915536
Unique Name	GR915536
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	307

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_ARATH (Phosphoenolpyruvate carboxykinase [ATP] OS=Arabidopsis thaliana GN=PCKA PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.561e-42
Identity = 48/55 (87.27%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTS+DLNLARREMVILGTQYAG+ +K +FSV
Sbjct:  285 GTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGEMKKGLFSV 339

HSP 2 Score: 92.0485 bits (227), Expect = 1.561e-42
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  331 EMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 377

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_MAIZE (Phosphoenolpyruvate carboxykinase [ATP] OS=Zea mays PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.870e-41
Identity = 45/50 (90.00%), Postives = 49/50 (98.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNLARREMVI+GTQYAG+ +K
Sbjct:  280 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLARREMVIMGTQYAGEMKK 329

HSP 2 Score: 89.3521 bits (220), Expect = 2.870e-41
Identity = 42/47 (89.36%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  326 EMKKGLFGVMHYLMPKRGILSLHSGCNMGKDGDVALFFGLSGTGKTT 372

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_CUCSA (Phosphoenolpyruvate carboxykinase [ATP] OS=Cucumis sativus GN=PCK PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.577e-39
Identity = 42/50 (84.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIY+AGQFPCNR+THYMTSSTSID+NL R+EMVILGTQYAG+ +K
Sbjct:  284 GTPDFTIYHAGQFPCNRYTHYMTSSTSIDMNLDRKEMVILGTQYAGEMKK 333

HSP 2 Score: 88.1965 bits (217), Expect = 2.577e-39
Identity = 41/47 (87.23%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFS+MHYLMP RQILSLHSGCNMGK+GDVALFFGLSGT   T
Sbjct:  330 EMKKGLFSLMHYLMPMRQILSLHSGCNMGKNGDVALFFGLSGTGKTT 376

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA2_UROPA (Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Urochloa panicoides GN=PCK2 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.635e-37
Identity = 41/47 (87.23%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKR ILSLHSGCNMGK+GDVALFFGLSGT   T
Sbjct:  286 EMKKGLFGVMHYLMPKRGILSLHSGCNMGKEGDVALFFGLSGTGKTT 332

HSP 2 Score: 85.8853 bits (211), Expect = 6.635e-37
Identity = 39/50 (78.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAG+FP NR+ +YMTSSTSI+++LARREMVILGTQYAG+ +K
Sbjct:  240 GTPDFTIYNAGEFPANRYANYMTSSTSINISLARREMVILGTQYAGEMKK 289

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA1_UROPA (Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Urochloa panicoides GN=PCK1 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.635e-37
Identity = 41/47 (87.23%), Postives = 42/47 (89.36%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKR ILSLHSGCNMGK+GDVALFFGLSGT   T
Sbjct:  284 EMKKGLFGVMHYLMPKRGILSLHSGCNMGKEGDVALFFGLSGTGKTT 330

HSP 2 Score: 85.8853 bits (211), Expect = 6.635e-37
Identity = 39/50 (78.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAG+FP NR+ +YMTSSTSI+++LARREMVILGTQYAG+ +K
Sbjct:  238 GTPDFTIYNAGEFPANRYANYMTSSTSINISLARREMVILGTQYAGEMKK 287

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA2_SALRD (Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Salinibacter ruber (strain DSM 13855 / M31) GN=pckA2 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 9.942e-28
Identity = 31/47 (65.96%), Postives = 38/47 (80.85%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKG+F+VMHYLMPK+ +LS+H   N G +GDV+LFFGLSGT   T
Sbjct:  194 EMKKGVFTVMHYLMPKKDVLSMHCSANEGDEGDVSLFFGLSGTGKTT 240

HSP 2 Score: 71.2478 bits (173), Expect = 9.942e-28
Identity = 35/58 (60.34%), Postives = 45/58 (77.59%), Query Frame = 3

Query:   15 ARAGTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            A  G PDF IYNAG+FP NR T +M+S TS+DL+   +EMVILGT+YAG+ +K VF+V
Sbjct:  145 ASFGEPDFVIYNAGEFPANRQTKHMSSKTSVDLSFENQEMVILGTEYAGEMKKGVFTV 202

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_DICDI (Phosphoenolpyruvate carboxykinase [ATP] OS=Dictyostelium discoideum GN=pckA PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.288e-25
Identity = 34/50 (68.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            G PD+TIYNAGQFP NR+T  M+SS+SI ++ AR+EMVILGTQYAG+ +K
Sbjct:  180 GEPDYTIYNAGQFPANRYTKGMSSSSSIAIDFARKEMVILGTQYAGEMKK 229

HSP 2 Score: 60.4622 bits (145), Expect = 3.288e-25
Identity = 28/48 (58.33%), Postives = 34/48 (70.83%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGK-DGDVALFFGLSGTSAAT 301
            EMKKG+ ++M YLMPK  +L LHS CN  + +GD  LFFGLSGT   T
Sbjct:  226 EMKKGILTIMMYLMPKMGVLPLHSSCNQARNNGDTTLFFGLSGTGKTT 273

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_NEUCR (Phosphoenolpyruvate carboxykinase [ATP] OS=Neurospora crassa GN=pck-1 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.279e-25
Identity = 33/53 (62.26%), Postives = 44/53 (83.02%), Query Frame = 3

Query:   30 PDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            PD+TIYNAG FP NR+T  M+SSTS+ +N A +EMVILGT+YAG+ +K +F+V
Sbjct:  180 PDYTIYNAGSFPANRYTEGMSSSTSVAINFAEKEMVILGTEYAGEMKKGIFTV 232

HSP 2 Score: 62.7734 bits (151), Expect = 4.279e-25
Identity = 32/48 (66.67%), Postives = 35/48 (72.92%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMP-KRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKG+F+VM Y  P K  IL+LHS  N GKDGDV LFFGLSGT   T
Sbjct:  224 EMKKGIFTVMFYEGPVKHNILTLHSSANEGKDGDVTLFFGLSGTGKTT 271

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_EMENI (Phosphoenolpyruvate carboxykinase [ATP] OS=Emericella nidulans GN=acuF PE=3 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.754e-24
Identity = 33/53 (62.26%), Postives = 43/53 (81.13%), Query Frame = 3

Query:   30 PDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            PD+ IYNAG FP NRFT  MTS+TS+ +N A +EMVILGT+YAG+ +K VF++
Sbjct:  175 PDYVIYNAGSFPANRFTEGMTSATSVAINFAEKEMVILGTEYAGEMKKGVFTI 227

HSP 2 Score: 58.9214 bits (141), Expect = 7.754e-24
Identity = 27/48 (56.25%), Postives = 36/48 (75.00%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMP-KRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKG+F+++ Y MP K  +L+LHS  N G++GDV +FFGLSGT   T
Sbjct:  219 EMKKGVFTILFYEMPVKHNVLTLHSSANEGQNGDVTVFFGLSGTGKTT 266

[Close]

BLAST of GR915536 vs. SwissProt
Match: PCKA_ASHGO (Phosphoenolpyruvate carboxykinase [ATP] OS=Ashbya gossypii GN=PCK1 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 6.372e-23
Identity = 31/58 (53.45%), Postives = 43/58 (74.14%), Query Frame = 3

Query:   15 ARAGTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            A  G PDFT++NAGQFP N  T  M+S T++++N    EMVILGT+YAG+ +K +F+V
Sbjct:  155 AEFGEPDFTVWNAGQFPANTHTEGMSSKTTVEINFRAMEMVILGTEYAGEMKKGIFTV 212

HSP 2 Score: 60.4622 bits (145), Expect = 6.372e-23
Identity = 29/48 (60.42%), Postives = 34/48 (70.83%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMP-KRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKG+F+VM YLMP    +L+LHS  N G + DV LFFGLSGT   T
Sbjct:  204 EMKKGIFTVMFYLMPINHNVLTLHSSANQGPNNDVTLFFGLSGTGKTT 251

[Close]

BLAST of GR915536 vs. TrEMBL
Match: B9SSD5_RICCO (Phosphoenolpyruvate carboxykinase [ATP], putative OS=Ricinus communis GN=RCOM_0188120 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 8.596e-42
Identity = 49/55 (89.09%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNLARREMVILGTQYAG+ +K +FSV
Sbjct:  280 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLARREMVILGTQYAGEMKKGLFSV 334

HSP 2 Score: 93.5893 bits (231), Expect = 8.596e-42
Identity = 44/47 (93.62%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  326 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTT 372

[Close]

BLAST of GR915536 vs. TrEMBL
Match: D7TZ44_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_1995.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005596001 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.467e-41
Identity = 49/55 (89.09%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNRFTHYMTSSTS+DLNLARREMVILGTQYAG+ +K +FSV
Sbjct:   17 GTPDFTIYNAGQFPCNRFTHYMTSSTSVDLNLARREMVILGTQYAGEMKKGLFSV 71

HSP 2 Score: 92.0485 bits (227), Expect = 1.467e-41
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKRQILSLHSGCN+GKDGDVALFFGLSGT   T
Sbjct:   63 EMKKGLFSVMHYLMPKRQILSLHSGCNIGKDGDVALFFGLSGTGKTT 109

[Close]

BLAST of GR915536 vs. TrEMBL
Match: B9HFR1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_719763 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.478e-41
Identity = 49/55 (89.09%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNLARREMVILGTQYAG+ +K +FSV
Sbjct:  278 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLARREMVILGTQYAGEMKKGLFSV 332

HSP 2 Score: 92.0485 bits (227), Expect = 2.478e-41
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  324 EMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 370

[Close]

BLAST of GR915536 vs. TrEMBL
Match: Q0WWL8_ARATH (Phosphoenolpyruvate carboxykinase (ATP)-like protein OS=Arabidopsis thaliana GN=At4g37870 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 3.229e-41
Identity = 48/55 (87.27%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTS+DLNLARREMVILGTQYAG+ +K +FSV
Sbjct:  285 GTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGEMKKGLFSV 339

HSP 2 Score: 92.0485 bits (227), Expect = 3.229e-41
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  331 EMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 377

[Close]

BLAST of GR915536 vs. TrEMBL
Match: Q56WR5_ARATH (Phosphoenolpyruvate carboxykinase-like protein OS=Arabidopsis thaliana GN=At4g37870 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 3.241e-41
Identity = 48/55 (87.27%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTS+DLNLARREMVILGTQYAG+ +K +FSV
Sbjct:   69 GTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGEMKKGLFSV 123

HSP 2 Score: 92.0485 bits (227), Expect = 3.241e-41
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  115 EMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 161

[Close]

BLAST of GR915536 vs. TrEMBL
Match: Q94LX9_FLATR (Phosphoenolpyruvate carboxykinase OS=Flaveria trinervia GN=pck4-9 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.580e-40
Identity = 45/50 (90.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNL RREMVILGTQYAG+ +K
Sbjct:  276 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLGRREMVILGTQYAGEMKK 325

HSP 2 Score: 92.0485 bits (227), Expect = 1.580e-40
Identity = 43/47 (91.49%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  322 EMKKGLFGVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTT 368

[Close]

BLAST of GR915536 vs. TrEMBL
Match: Q94LX8_FLATR (Phosphoenolpyruvate carboxykinase OS=Flaveria trinervia GN=pck3-19 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.580e-40
Identity = 45/50 (90.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNL RREMVILGTQYAG+ +K
Sbjct:  276 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLGRREMVILGTQYAGEMKK 325

HSP 2 Score: 92.0485 bits (227), Expect = 1.580e-40
Identity = 43/47 (91.49%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  322 EMKKGLFGVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTT 368

[Close]

BLAST of GR915536 vs. TrEMBL
Match: Q94LX7_FLAPR (Phosphoenolpyruvate carboxykinase OS=Flaveria pringlei GN=pck3-11 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.580e-40
Identity = 45/50 (90.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNR+THYMTSSTSIDLNL RREMVILGTQYAG+ +K
Sbjct:  272 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDLNLGRREMVILGTQYAGEMKK 321

HSP 2 Score: 92.0485 bits (227), Expect = 1.580e-40
Identity = 43/47 (91.49%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  318 EMKKGLFGVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTT 364

[Close]

BLAST of GR915536 vs. TrEMBL
Match: B8ZV66_PHRAU (Phosphoenolpyruvate carboxykinase (Fragment) OS=Phragmites australis GN=pck PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.585e-40
Identity = 45/50 (90.00%), Postives = 49/50 (98.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNRFTHYMTSSTS+D+NLARREMVILGTQYAG+ +K
Sbjct:  137 GTPDFTIYNAGQFPCNRFTHYMTSSTSVDINLARREMVILGTQYAGEMKK 186

HSP 2 Score: 90.5077 bits (223), Expect = 1.585e-40
Identity = 42/47 (89.36%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  183 EMKKGLFGVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 229

[Close]

BLAST of GR915536 vs. TrEMBL
Match: B9GN56_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816402 PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.059e-40
Identity = 44/50 (88.00%), Postives = 49/50 (98.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAGQFPCNR+THYMTSSTSID+NLAR+EMVILGTQYAG+ +K
Sbjct:  280 GTPDFTIYNAGQFPCNRYTHYMTSSTSIDINLARKEMVILGTQYAGEMKK 329

HSP 2 Score: 92.0485 bits (227), Expect = 2.059e-40
Identity = 43/47 (91.49%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  326 EMKKGLFGVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTT 372

[Close]

BLAST of GR915536 vs. TAIR peptide
Match: AT4G37870.1 (| Symbols: PCK1, PEPCK | phosphoenolpyruvate carboxykinase 1 | chr4:17802974-17806332 REVERSE LENGTH=671)

HSP 1 Score: 100.523 bits (249), Expect = 1.232e-43
Identity = 48/55 (87.27%), Postives = 53/55 (96.36%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK-VFSV 185
            GTPDFTIYNAGQFPCNR+THYMTSSTS+DLNLARREMVILGTQYAG+ +K +FSV
Sbjct:  285 GTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGEMKKGLFSV 339

HSP 2 Score: 92.0485 bits (227), Expect = 1.232e-43
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLFSVMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  331 EMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTT 377

[Close]

BLAST of GR915536 vs. TAIR peptide
Match: AT5G65690.1 (| Symbols: PCK2, PEPCK | phosphoenolpyruvate carboxykinase 2 | chr5:26266477-26269486 FORWARD LENGTH=670)

HSP 1 Score: 96.6709 bits (239), Expect = 5.006e-42
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:   24 GTPDFTIYNAGQFPCNRFTHYMTSSTSIDLNLARREMVILGTQYAGK*RK 173
            GTPDFTIYNAG+FPCNRFTHYMTSSTS+D+NL RREMVILGTQYAG+ +K
Sbjct:  284 GTPDFTIYNAGKFPCNRFTHYMTSSTSVDINLGRREMVILGTQYAGEMKK 333

HSP 2 Score: 90.5077 bits (223), Expect = 5.006e-42
Identity = 42/47 (89.36%), Postives = 43/47 (91.49%), Query Frame = 2

Query:  161 EMKKGLFSVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTSAAT 301
            EMKKGLF VMHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGT   T
Sbjct:  330 EMKKGLFGVMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTT 376

The following BLAST results are available for this feature:

BLAST of GR915536 vs. SwissProt
BLAST of GR915536 vs. TrEMBL
BLAST of GR915536 vs. TAIR peptide

BLAST of GR915536 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PCKA_ARATH	1.561e-42	87.27	Phosphoenolpyruvate carboxykinase [ATP] OS=Arabido...	[more]
PCKA_MAIZE	2.870e-41	90.00	Phosphoenolpyruvate carboxykinase [ATP] OS=Zea may...	[more]
PCKA_CUCSA	2.577e-39	84.00	Phosphoenolpyruvate carboxykinase [ATP] OS=Cucumis...	[more]
PCKA2_UROPA	6.635e-37	87.23	Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Uroch...	[more]
PCKA1_UROPA	6.635e-37	87.23	Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Uroch...	[more]
PCKA2_SALRD	9.942e-28	65.96	Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Salin...	[more]
PCKA_DICDI	3.288e-25	68.00	Phosphoenolpyruvate carboxykinase [ATP] OS=Dictyos...	[more]
PCKA_NEUCR	4.279e-25	62.26	Phosphoenolpyruvate carboxykinase [ATP] OS=Neurosp...	[more]
PCKA_EMENI	7.754e-24	62.26	Phosphoenolpyruvate carboxykinase [ATP] OS=Emerice...	[more]
PCKA_ASHGO	6.372e-23	53.45	Phosphoenolpyruvate carboxykinase [ATP] OS=Ashbya ...	[more]

BLAST of GR915536 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SSD5_RICCO	8.596e-42	89.09	Phosphoenolpyruvate carboxykinase [ATP], putative ...	[more]
D7TZ44_VITVI	1.467e-41	89.09	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HFR1_POPTR	2.478e-41	89.09	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q0WWL8_ARATH	3.229e-41	87.27	Phosphoenolpyruvate carboxykinase (ATP)-like prote...	[more]
Q56WR5_ARATH	3.241e-41	87.27	Phosphoenolpyruvate carboxykinase-like protein OS=...	[more]
Q94LX9_FLATR	1.580e-40	90.00	Phosphoenolpyruvate carboxykinase OS=Flaveria trin...	[more]
Q94LX8_FLATR	1.580e-40	90.00	Phosphoenolpyruvate carboxykinase OS=Flaveria trin...	[more]
Q94LX7_FLAPR	1.580e-40	90.00	Phosphoenolpyruvate carboxykinase OS=Flaveria prin...	[more]
B8ZV66_PHRAU	1.585e-40	90.00	Phosphoenolpyruvate carboxykinase (Fragment) OS=Ph...	[more]
B9GN56_POPTR	2.059e-40	88.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GR915536 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT4G37870.1	1.232e-43	87.27	\| Symbols: PCK1, PEPCK \| phosphoenolpyruvate carbo...	[more]
AT5G65690.1	5.006e-42	86.00	\| Symbols: PCK2, PEPCK \| phosphoenolpyruvate carbo...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001272	Phosphoenolpyruvate carboxykinase, ATP-utilising	PFAM	PF01293	PEPCK_ATP	coord: 12..57 score: 9
IPR001272	Phosphoenolpyruvate carboxykinase, ATP-utilising	PFAM	PF01293	PEPCK_ATP	coord: 8..53 score: 7.2
IPR008210	Phosphoenolpyruvate carboxykinase, N-terminal	GENE3D	3.40.449.10	no description	coord: 13..29 score: 0.
IPR008210	Phosphoenolpyruvate carboxykinase, N-terminal	GENE3D	3.40.449.10	no description	coord: 6..53 score: 1.1
IPR008210	Phosphoenolpyruvate carboxykinase, N-terminal	SUPERFAMILY	68923	PEP carboxykinase N-terminal domain	coord: 8..53 score: 4.3
IPR013035	Phosphoenolpyruvate carboxykinase, C-terminal	GENE3D	3.90.228.20	no description	coord: 31..59 score: 1.2
None	No IPR available	SUPERFAMILY	53795	PEP carboxykinase-like	coord: 31..55 score: 7.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR915536 ID=GR915536; Name=GR915536; organism=Cicer arietinum; type=EST; length=307bp
GATTTCGAGCGGCCGCCCGGGCAGGTACTCCAGACTTCACTATATACAAT
GCCGGGCAGTTTCCATGTAATCGTTTCACTCACTACATGACATCTTCCAC
TAGCATTGATCTTAATCTTGCTAGAAGGGAAATGGTTATCCTCGGCACGC
AGTATGCCGGGAAATGAAGAAAGGTCTTTTCAGTGTCATGCATTATCTCA
TGCCTAAGCGCCAAATTCTCTCCTTACACTCTGGTTGCAATATGGGCAAA
GATGGTGATGTTGCACTCTTCTTTGGACTCTCAGGTACCTCGGCCGCGAC
CACGCTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004612	phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005524	ATP binding
GO:0004611	phosphoenolpyruvate carboxykinase activity
GO:0017076	purine nucleotide binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006094	gluconeogenesis

Vocabulary: **INTERPRO**
Term	Definition
IPR001272	PEP_carboxykinase_ATP
IPR008210	PEP_carboxykinase_N
IPR013035	PEP_carboxykinase_C

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