GR917101

EST Overview


Name	GR917101
Unique Name	GR917101
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	405

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR917101 vs. SwissProt
Match: GOX_SPIOL (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.152e-49
Identity = 98/127 (77.17%), Postives = 103/127 (81.10%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRF +PP L LKNFE               +SYV+GQ+DRSL+WKDV WLQTITSLPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLSSYVAGQIDRSLSWKDVAWLQTITSLPILVKG 231

[Close]

BLAST of GR917101 vs. SwissProt
Match: GOX2_ARATH (Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 OS=Arabidopsis thaliana GN=At3g14420 PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 4.590e-48
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. SwissProt
Match: GOX1_ARATH (Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 OS=Arabidopsis thaliana GN=At3g14415 PE=1 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 2.975e-47
Identity = 93/127 (73.23%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+R VV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKD++WLQTIT++PILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDIQWLQTITNMPILVKG 231

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX1_HUMAN (Hydroxyacid oxidase 1 OS=Homo sapiens GN=HAO1 PE=1 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 5.099e-31
Identity = 68/136 (50.00%), Postives = 90/136 (66.18%), Query Frame = 1

Query:   10 GRGRGLSSWPTSSVEEVASTGP-GIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFE---XXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            G G  LSSW TSS+EEVA  GP  +R+ QLY+ KDR V  +LVR+AE  G+KAI +TVDTP LG R  D++NRF +PP L +KNFE                  A+YV+  +D S++W+D+KWL+ +TSLPI+ KG
Sbjct:  102 GTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKG 237

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX1_MOUSE (Hydroxyacid oxidase 1 OS=Mus musculus GN=Hao1 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 1.136e-30
Identity = 68/136 (50.00%), Postives = 88/136 (64.71%), Query Frame = 1

Query:   10 GRGRGLSSWPTSSVEEVASTGP-GIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFE---XXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            G G  LSSW TSS+EEVA  GP  +R+ QLY+ KDR +  Q+V+RAE  G+KAI +TVDTP LG R  D++NRF +PP L +KNFE                  A YV+  +D SL+W D+ WL+ +TSLPI+VKG
Sbjct:  102 GTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKG 237

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX_DICDI (Hydroxyacid oxidase OS=Dictyostelium discoideum GN=haox PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.201e-25
Identity = 65/130 (50.00%), Postives = 82/130 (63.08%), Query Frame = 1

Query:   25 LSSWPTSSVEEVAST---GPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSS  T+SVE+++S     PG  +FQLYV KDR V  +LV+RAES G+ A+ LTVDTP LG+R AD KN F +P  L LK FE                 Y++  +D SL W D+KWL++IT LPILVKG
Sbjct:  133 LSSLSTTSVEDLSSATNGNPG--WFQLYVFKDRKVSEELVKRAESIGYSALVLTVDTPFLGKRTADFKNSFKLPNGLSLKIFEKLMLSNLDGGLN----QYIATMIDPSLTWNDLKWLKSITKLPILVKG 256

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX2_BOVIN (Hydroxyacid oxidase 2 OS=Bos taurus GN=HAO2 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 9.335e-17
Identity = 43/127 (33.86%), Postives = 74/127 (58.27%), Query Frame = 1

Query:   28 SSWPTSSVEEVASTGP-GIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            S++ + S+E++ +  P G+R+FQLYV  +R +  Q++++ ES GFKA+ +TVD P +G R  DI N+  +   L+LK+                   +    +D S+ W+D+ W Q++T LPI++KG
Sbjct:  106 STYASCSLEDIVAAAPRGLRWFQLYVHPNRQINKQMIQKVESLGFKALVITVDVPKVGNRRNDITNQVDLMKKLLLKDL-------GSPEMGNVMPYFQMSPIDPSICWEDLSWFQSMTRLPIILKG 225

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX2_RAT (Hydroxyacid oxidase 2 OS=Rattus norvegicus GN=Hao2 PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 3.922e-15
Identity = 45/128 (35.16%), Postives = 72/128 (56.25%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGP-GIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            +SS+ + S+E++ +  P G R+FQLY+  D +   Q+V+RAE+ GFKA+ +T+DTP+LG R  D +N+  +   ++LK+                  S        S  W D+  LQ+IT LPI++KG
Sbjct:  105 ISSYASYSLEDIVAAAPEGFRWFQLYMKSDWDFNKQMVQRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILLKDLR-------ALKEEKPTQSVPVSFPKASFCWNDLSLLQSITRLPIILKG 225

[Close]

BLAST of GR917101 vs. SwissProt
Match: HAOX2_HUMAN (Hydroxyacid oxidase 2 OS=Homo sapiens GN=HAO2 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.690e-15
Identity = 45/127 (35.43%), Postives = 70/127 (55.12%), Query Frame = 1

Query:   28 SSWPTSSVEEVASTGP-GIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            S++ + S+E++    P G+R+FQLYV  D  +  QL++R ES GFKA+ +T+DTP+ G R  DI+N+      L L + +                 +    +  SL W D+ W Q+IT LPI++KG
Sbjct:  106 STFASCSLEDIVIAAPEGLRWFQLYVHPDLQLNKQLIQRVESLGFKALVITLDTPVCGNRRHDIRNQLRRN--LTLTDLQSPKKGNAIPY-------FQMTPISTSLCWNDLSWFQSITRLPIILKG 223

[Close]

BLAST of GR917101 vs. SwissProt
Match: CYB2_YEAST (Cytochrome b2, mitochondrial OS=Saccharomyces cerevisiae GN=CYB2 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 9.660e-14
Identity = 50/147 (34.01%), Postives = 75/147 (51.02%), Query Frame = 1

Query:    7 RGRGRGLSSWP-------TSSVEEVASTGPG---IRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTM----PPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            RG G+G++  P       + S EE+    P    I+++QLYV  DR +   LV+  E  G KA+ +TVD P LG+RE D+K +F+     P  +   N E               +  +S  +D SL WKD++ L+  T LPI++KG
Sbjct:  294 RGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNTKAGPKAMKKTNVE----------ESQGASRALSKFIDPSLTWKDIEELKKKTKLPIVIKG 430

[Close]

BLAST of GR917101 vs. TrEMBL
Match: C6TBK3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 3.425e-49
Identity = 100/127 (78.74%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP+LGRREADIKNRFT+PP L LKNFE               ASYVSGQ+DR+L+WKDVKWLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPILGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKADDSGLASYVSGQIDRTLSWKDVKWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: B0M1A2_SOYBN (Peroxisomal glycolate oxidase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 3.425e-49
Identity = 100/127 (78.74%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP+LGRREADIKNRFT+PP L LKNFE               ASYVSGQ+DR+L+WKDVKWLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPILGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKADDSGLASYVSGQIDRTLSWKDVKWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: P93260_MESCR (Glycolate oxidase OS=Mesembryanthemum crystallinum GN=GOX PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 9.966e-49
Identity = 100/127 (78.74%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DRSL+WKDVKWLQTITSLPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGTMDKADDSGLASYVAGQIDRSLSWKDVKWLQTITSLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: B0M1B1_SOYBN (Peroxisomal glycolate oxidase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.302e-48
Identity = 99/127 (77.95%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDVKWLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKADDSGLASYVAGQIDRTLSWKDVKWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: Q84LB8_ZANAE (Glycolate oxidase OS=Zantedeschia aethiopica GN=gox PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.700e-48
Identity = 99/127 (77.95%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DRSL+WKDVKWLQTITS+PILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPHLTLKNFEGLDLGKMDKSNDSGLASYVAGQIDRSLSWKDVKWLQTITSMPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: D7TKA1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021520001 PE=4 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.220e-48
Identity = 99/127 (77.95%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDR+VV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DRSL+WKDVKWLQTIT LPILVKG
Sbjct:  108 LSSWATSSVEEVASTGPGIRFFQLYVYKDRHVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKADDSGLASYVAGQIDRSLSWKDVKWLQTITKLPILVKG 234

[Close]

BLAST of GR917101 vs. TrEMBL
Match: Q39640_9ROSI (Glycolate oxidase OS=Cucurbita cv. Kurokawa Amakuri PE=2 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.900e-48
Identity = 98/127 (77.17%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+W+DVKWLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDQADDSGLASYVAGQIDRTLSWQDVKWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: B9S0Y9_RICCO ((S)-2-hydroxy-acid oxidase, putative OS=Ricinus communis GN=RCOM_0631490 PE=4 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.900e-48
Identity = 97/127 (76.38%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV +DRNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               +SYV+GQ+DR+L+WKD+KWLQTITSLPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYRDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKSDDSGLSSYVAGQIDRTLSWKDIKWLQTITSLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: D7TQZ8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_93.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00038078001 PE=4 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.787e-48
Identity = 98/127 (77.17%), Postives = 105/127 (82.68%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDR+VV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDVKWLQTIT+LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRHVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKADDSGLASYVAGQIDRTLSWKDVKWLQTITNLPILVKG 231

[Close]

BLAST of GR917101 vs. TrEMBL
Match: A9PFI8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_583055 PE=2 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.787e-48
Identity = 98/127 (77.17%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV KDRNVV QLVRRAE AGFKAIALTVDTP LGRREADIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKAADSGLASYVAGQIDRTLSWKDVEWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.6 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821899-4823899 FORWARD LENGTH=366)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  104 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 230

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.5 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821829-4823899 FORWARD LENGTH=360)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:   98 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 224

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.4 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821861-4823899 FORWARD LENGTH=348)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:   86 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 212

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.2 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821804-4823899 FORWARD LENGTH=367)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.3 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821804-4823899 FORWARD LENGTH=367)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14420.1 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4821804-4823899 FORWARD LENGTH=367)

HSP 1 Score: 189.504 bits (480), Expect = 5.206e-49
Identity = 96/127 (75.59%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+RNVV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKDV+WLQTIT LPILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14415.3 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4818667-4820748 FORWARD LENGTH=367)

HSP 1 Score: 186.808 bits (473), Expect = 3.374e-48
Identity = 93/127 (73.23%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+R VV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKD++WLQTIT++PILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDIQWLQTITNMPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT3G14415.1 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:4818667-4820748 FORWARD LENGTH=367)

HSP 1 Score: 186.808 bits (473), Expect = 3.374e-48
Identity = 93/127 (73.23%), Postives = 104/127 (81.89%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW TSSVEEVASTGPGIRFFQLYV K+R VV QLVRRAE AGFKAIALTVDTP LGRRE+DIKNRFT+PP L LKNFE               ASYV+GQ+DR+L+WKD++WLQTIT++PILVKG
Sbjct:  105 LSSWATSSVEEVASTGPGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDIQWLQTITNMPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT4G18360.2 (| Symbols: | Aldolase-type TIM barrel family protein | chr4:10146627-10148386 REVERSE LENGTH=314)

HSP 1 Score: 184.111 bits (466), Expect = 2.187e-47
Identity = 94/127 (74.02%), Postives = 102/127 (80.31%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW T SVEEVASTGPGIRFFQLYV KDRNVV QLV+RAE AGFKAIALTVDTP LGRRE+DIKNRF +P  L LKNFE               ASYV+GQVD+SL+WKD+KWLQ+ITSLPILVKG
Sbjct:  105 LSSWATCSVEEVASTGPGIRFFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRESDIKNRFALPRGLTLKNFEGLDLGKIDKTNDSGLASYVAGQVDQSLSWKDIKWLQSITSLPILVKG 231

[Close]

BLAST of GR917101 vs. TAIR peptide
Match: AT4G18360.1 (| Symbols: | Aldolase-type TIM barrel family protein | chr4:10146141-10148386 REVERSE LENGTH=368)

HSP 1 Score: 184.111 bits (466), Expect = 2.187e-47
Identity = 94/127 (74.02%), Postives = 102/127 (80.31%), Query Frame = 1

Query:   25 LSSWPTSSVEEVASTGPGIRFFQLYVVKDRNVVTQLVRRAESAGFKAIALTVDTPMLGRREADIKNRFTMPPLLVLKNFEXXXXXXXXXXXXXXXASYVSGQVDRSLNWKDVKWLQTITSLPILVKG 405
            LSSW T SVEEVASTGPGIRFFQLYV KDRNVV QLV+RAE AGFKAIALTVDTP LGRRE+DIKNRF +P  L LKNFE               ASYV+GQVD+SL+WKD+KWLQ+ITSLPILVKG
Sbjct:  105 LSSWATCSVEEVASTGPGIRFFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRESDIKNRFALPRGLTLKNFEGLDLGKIDKTNDSGLASYVAGQVDQSLSWKDIKWLQSITSLPILVKG 231

The following BLAST results are available for this feature:

BLAST of GR917101 vs. SwissProt
BLAST of GR917101 vs. TrEMBL
BLAST of GR917101 vs. TAIR peptide

BLAST of GR917101 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GOX_SPIOL	4.152e-49	77.17	Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia...	[more]
GOX2_ARATH	4.590e-48	75.59	Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 ...	[more]
GOX1_ARATH	2.975e-47	73.23	Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 ...	[more]
HAOX1_HUMAN	5.099e-31	50.00	Hydroxyacid oxidase 1 OS=Homo sapiens GN=HAO1 PE=1...	[more]
HAOX1_MOUSE	1.136e-30	50.00	Hydroxyacid oxidase 1 OS=Mus musculus GN=Hao1 PE=2...	[more]
HAOX_DICDI	3.201e-25	50.00	Hydroxyacid oxidase OS=Dictyostelium discoideum GN...	[more]
HAOX2_BOVIN	9.335e-17	33.86	Hydroxyacid oxidase 2 OS=Bos taurus GN=HAO2 PE=2 S...	[more]
HAOX2_RAT	3.922e-15	35.16	Hydroxyacid oxidase 2 OS=Rattus norvegicus GN=Hao2...	[more]
HAOX2_HUMAN	6.690e-15	35.43	Hydroxyacid oxidase 2 OS=Homo sapiens GN=HAO2 PE=2...	[more]
CYB2_YEAST	9.660e-14	34.01	Cytochrome b2, mitochondrial OS=Saccharomyces cere...	[more]

BLAST of GR917101 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TBK3_SOYBN	3.425e-49	78.74	Putative uncharacterized protein OS=Glycine max PE...	[more]
B0M1A2_SOYBN	3.425e-49	78.74	Peroxisomal glycolate oxidase OS=Glycine max PE=2 ...	[more]
P93260_MESCR	9.966e-49	78.74	Glycolate oxidase OS=Mesembryanthemum crystallinum...	[more]
B0M1B1_SOYBN	1.302e-48	77.95	Peroxisomal glycolate oxidase OS=Glycine max PE=2 ...	[more]
Q84LB8_ZANAE	1.700e-48	77.95	Glycolate oxidase OS=Zantedeschia aethiopica GN=go...	[more]
D7TKA1_VITVI	2.220e-48	77.95	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q39640_9ROSI	2.900e-48	77.17	Glycolate oxidase OS=Cucurbita cv. Kurokawa Amakur...	[more]
B9S0Y9_RICCO	2.900e-48	76.38	(S)-2-hydroxy-acid oxidase, putative OS=Ricinus co...	[more]
D7TQZ8_VITVI	3.787e-48	77.17	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9PFI8_POPTR	3.787e-48	77.17	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GR917101 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G14420.6	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14420.5	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14420.4	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14420.2	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14420.3	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14420.1	5.206e-49	75.59	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14415.3	3.374e-48	73.23	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G14415.1	3.374e-48	73.23	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT4G18360.2	2.187e-47	74.02	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT4G18360.1	2.187e-47	74.02	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 6..133 score: 3.2
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	PROFILE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 1..133 score: 38
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	no description	coord: 6..133 score: 4.3
None	No IPR available	PANTHER	PTHR10578	(S)-2-HYDROXY-ACID OXIDASE-RELATED	coord: 7..133 score: 9
None	No IPR available	PANTHER	PTHR10578:SF9	(S)-2-HYDROXY-ACID OXIDASE	coord: 7..133 score: 9
None	No IPR available	SEG	seg	seg	coord: 87..101 score
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 2..133 score: 3.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR917101 ID=GR917101; Name=GR917101; organism=Cicer arietinum; type=EST; length=405bp
GATTAGCGTGGTCGCGGCCGAGGTCTATCCTCTTGGCCTACTTCCAGTGT
TGAGGAAGTTGCTTCAACAGGACCTGGCATTCGCTTTTTCCAACTCTACG
TGGTTAAAGATAGAAATGTGGTTACTCAGCTTGTGAGAAGAGCTGAATCT
GCCGGTTTCAAGGCAATTGCCCTTACTGTGGACACTCCAATGCTTGGTCG
CAGGGAGGCTGATATTAAAAACAGATTTACAATGCCACCTCTCCTTGTAT
TGAAAAACTTTGAAGGATTGGATCTTGGGAAGCTGGATAAGACTGATGAC
TCTGGTCTAGCCTCTTATGTTTCTGGCCAAGTTGACCGATCACTCAATTG
GAAGGATGTGAAATGGCTCCAAACAATCACCTCATTGCCAATTTTAGTGA
AGGGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0010181	FMN binding
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000262	FMN-dep_DH
IPR012133	Alpha-hydoxy_acid_DH_FMN
IPR013785	Aldolase_TIM

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