HO063888

EST Overview


Name	HO063888
Unique Name	HO063888
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	584

Libraries


Library Name	Type
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_ARATH (Proteasome subunit beta type-4 OS=Arabidopsis thaliana GN=PBG1 PE=1 SV=2)

HSP 1 Score: 166.007 bits (419), Expect = 1.233e-40
Identity = 77/90 (85.56%), Postives = 85/90 (94.44%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV G+S+VAIKYKDG+LMA+DMGGSYGSTLRYK++ER K IGKHSLLGASGEISDFQEI+RYLDEL LNDNMWDDGNSLGPKE+ NYL
Sbjct:   26 YPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYL 115

HSP 2 Score: 65.855 bits (159), Expect = 1.736e-10
Identity = 28/38 (73.68%), Postives = 32/38 (84.21%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG TV  P+SLKTYWEFS+F NPT GA GSW
Sbjct:  209 QIAKITEEGVTVSQPYSLKTYWEFSSFHNPTAGAAGSW 246

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_DICDI (Proteasome subunit beta type-4 OS=Dictyostelium discoideum GN=psmB4-1 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 44/89 (49.44%), Postives = 62/89 (69.66%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+AIKY  G++M +DM  SYGS  R+ S ER K  G ++++GASGE SDFQ I   L++L+ +D+  DDG+ L  +E+ NYL
Sbjct:   40 PIVTGTSVIAIKYDKGVVMGSDMLLSYGSLARFNSTERMKKFGGYTIVGASGEYSDFQSITNTLNDLVTDDHCMDDGSKLSSEEIWNYL 128

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_SCHPO (Probable proteasome subunit beta type-4 OS=Schizosaccharomyces pombe GN=SPBC577.10 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.621e-16
Identity = 44/103 (42.72%), Postives = 67/103 (65.05%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYLKLPKKVLLFSRHSL 352
            P V GSSV+A+K+ DG+++AAD   SYGS  R+  VER   +G ++++GA G+ISD+Q+I R L++L + +  + DG +L P  +  YL    KVL   R+ L
Sbjct:   39 PIVTGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLLEKLEIKEGNYGDGYALQPSYIHEYL---SKVLYARRNKL 138

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_XENLA (Proteasome subunit beta type-4 (Fragment) OS=Xenopus laevis GN=psmb4 PE=2 SV=2)

HSP 1 Score: 80.8777 bits (198), Expect = 5.215e-15
Identity = 31/89 (34.83%), Postives = 65/89 (73.03%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K+  G+++AADM GSYGS  R++++ R   + ++++LGASG+ +D+Q + + +D++++++ +  DG++  PK + ++L
Sbjct:   27 PMVTGTSVLGVKFDGGVIIAADMLGSYGSLARFRNISRIMKVNENTILGASGDYADYQYLKQVIDQMVIDEELVGDGHNYSPKAIHSWL 115

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_PIG (Proteasome subunit beta type-4 (Fragment) OS=Sus scrofa GN=PSMB4 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.811e-15
Identity = 33/89 (37.08%), Postives = 63/89 (70.79%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K++ G+++AADM GSYGS  R++++ R   +   ++LGASG+ +DFQ + + L ++++++ +  DG+S  PK + ++L
Sbjct:   49 PMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWL 137

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_BOVIN (Proteasome subunit beta type-4 OS=Bos taurus GN=PSMB4 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.896e-15
Identity = 33/89 (37.08%), Postives = 63/89 (70.79%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K++ G+++AADM GSYGS  R++++ R   +   ++LGASG+ +DFQ + + L ++++++ +  DG+S  PK + ++L
Sbjct:   49 PMVTGTSVLGLKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWL 137

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_HUMAN (Proteasome subunit beta type-4 OS=Homo sapiens GN=PSMB4 PE=1 SV=4)

HSP 1 Score: 79.337 bits (194), Expect = 1.517e-14
Identity = 32/89 (35.96%), Postives = 63/89 (70.79%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K++ G+++AADM GSYGS  R++++ R   +   ++LGASG+ +DFQ + + L ++++++ +  DG+S  P+ + ++L
Sbjct:   49 PMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWL 137

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_RAT (Proteasome subunit beta type-4 OS=Rattus norvegicus GN=Psmb4 PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 2.588e-14
Identity = 32/89 (35.96%), Postives = 63/89 (70.79%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K+  G+++AADM GSYGS  R++++ R   +   ++LGASG+ +DFQ + + L ++++++ ++ DG+S  P+ + ++L
Sbjct:   48 PMVTGTSVLGVKFDCGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGASGDYADFQYLKQVLGQMVIDEELFGDGHSYSPRAIHSWL 136

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_MOUSE (Proteasome subunit beta type-4 OS=Mus musculus GN=Psmb4 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.381e-14
Identity = 32/89 (35.96%), Postives = 62/89 (69.66%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            P V G+SV+ +K+  G+++AADM GSYGS  R++++ R   +   ++LGASG+ +DFQ + + L ++++++ +  DG+S  P+ + ++L
Sbjct:   49 PMVTGTSVLGVKFDGGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWL 137

[Close]

BLAST of HO063888 vs. SwissProt
Match: PSB4_YEAST (Proteasome component PRE4 OS=Saccharomyces cerevisiae GN=PRE4 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 4.415e-14
Identity = 41/93 (44.09%), Postives = 61/93 (65.59%), Query Frame = 2

Query:   44 PYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILN---DN-MWDDGNSLGPKEVLNYL 310
            P V G+SV+++KY +G+++AAD  GSYGS LR+  VER  P+G ++++G SG+ISD Q I R L +L+     DN + D   +L P  +  YL
Sbjct:   37 PIVTGTSVISMKYDNGVIIAADNLGSYGSLLRFNGVERLIPVGDNTVVGISGDISDMQHIERLLKDLVTENAYDNPLADAEEALEPSYIFEYL 129

[Close]

BLAST of HO063888 vs. TrEMBL
Match: B9IKM7_POPTR (Proteasome subunit beta type OS=Populus trichocarpa GN=POPTRDRAFT_737038 PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 2.786e-42
Identity = 85/104 (81.73%), Postives = 93/104 (89.42%), Query Frame = 2

Query:   11 NSISSGRPG----RYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            NS+ S  PG    +YPYV GSSVVA+KYKDGILMAADMGGSYGSTLRYKSVER KP+GKHSL+GASGEISDFQEIMRYLDE +LNDNMWDDGNSLGPKE+ +YL
Sbjct:   10 NSLLSSEPGIQRTQYPYVTGSSVVALKYKDGILMAADMGGSYGSTLRYKSVERIKPVGKHSLIGASGEISDFQEIMRYLDEQVLNDNMWDDGNSLGPKEIHSYL 113

HSP 2 Score: 62.7734 bits (151), Expect = 2.015e-8
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG T+  P++LKT+W F AF+NPT GAEGSW
Sbjct:  207 QIAKITEEGVTISQPYALKTFWGFKAFENPTAGAEGSW 244

[Close]

BLAST of HO063888 vs. TrEMBL
Match: B7FJ04_MEDTR (Proteasome subunit beta type OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 6.207e-42
Identity = 86/100 (86.00%), Postives = 91/100 (91.00%), Query Frame = 2

Query:   11 NSISSGRPGRYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            ++++  +   YPYV GSSVVAIKYKDGILMAADMGGSYGSTLRYKSVER KPIGKHSLLGASGEISDFQEIMRYLDELIL DNMWDDGNSLGPKEV NYL
Sbjct:    2 DTVTESQRTLYPYVTGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERLKPIGKHSLLGASGEISDFQEIMRYLDELILIDNMWDDGNSLGPKEVHNYL 101

HSP 2 Score: 73.9442 bits (180), Expect = 1.736e-15
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAE 405
            Q+SKITEEGATV+PPFSLKTYWEFSAFKNPTVGAE
Sbjct:  195 QISKITEEGATVYPPFSLKTYWEFSAFKNPTVGAE 229

HSP 3 Score: 32.7278 bits (73), Expect = 1.736e-15
Identity = 14/19 (73.68%), Postives = 15/19 (78.95%), Query Frame = 2

Query:  404 KVHGSLLVANEMIPNFKWI 460
            +VHGSLLV NEM  NF WI
Sbjct:  229 EVHGSLLVVNEMKQNFTWI 247

[Close]

BLAST of HO063888 vs. TrEMBL
Match: C6T3I7_SOYBN (Proteasome subunit beta type OS=Glycine max PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 1.383e-41
Identity = 85/90 (94.44%), Postives = 86/90 (95.56%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV GSSVVAIKYKDGILMAADMGGSYGSTLRYKSVER KPIGKHSLLGASGEISDFQEI+RYLDELIL DNMWDDGNSLGPKEV NYL
Sbjct:   12 YPYVTGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERIKPIGKHSLLGASGEISDFQEILRYLDELILYDNMWDDGNSLGPKEVHNYL 101

HSP 2 Score: 78.5666 bits (192), Expect = 3.549e-13
Identity = 35/38 (92.11%), Postives = 37/38 (97.37%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q+SKIT+EGATVFPPFSLKTYWEFSAFKNPTVGAE SW
Sbjct:  195 QISKITDEGATVFPPFSLKTYWEFSAFKNPTVGAECSW 232

[Close]

BLAST of HO063888 vs. TrEMBL
Match: D7KJN2_ARALY (20S proteasome beta subunit PBG1 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_334205 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 4.024e-41
Identity = 83/103 (80.58%), Postives = 92/103 (89.32%), Query Frame = 2

Query:    2 GRGNSISSGRPGRYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            G  N+ +  +   YPYV G+SVVAIKYKDGILMA+DMGGSYGSTLRYK++ER K IGKHSLLGASGEISDFQEI+RYLDEL+LNDNMWDDGNSLGPKEVLNYL
Sbjct:   12 GVKNAEADSQRTLYPYVTGTSVVAIKYKDGILMASDMGGSYGSTLRYKNIERMKAIGKHSLLGASGEISDFQEILRYLDELMLNDNMWDDGNSLGPKEVLNYL 114

HSP 2 Score: 65.4698 bits (158), Expect = 3.109e-9
Identity = 27/38 (71.05%), Postives = 33/38 (86.84%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++K+TEEG TV  P+SLKT+W+F AF NPTVGAEGSW
Sbjct:  208 QIAKMTEEGVTVSEPYSLKTFWDFKAFTNPTVGAEGSW 245

[Close]

BLAST of HO063888 vs. TrEMBL
Match: C6SWQ4_SOYBN (Proteasome subunit beta type OS=Glycine max PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 4.024e-41
Identity = 84/90 (93.33%), Postives = 86/90 (95.56%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV GSSVVAIKYKDGILMAADMGGSYGSTLRYKSVER KPIGKHSLLGASGEISDFQEI+RYLDELIL DNMW+DGNSLGPKEV NYL
Sbjct:   12 YPYVTGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERIKPIGKHSLLGASGEISDFQEILRYLDELILYDNMWEDGNSLGPKEVHNYL 101

HSP 2 Score: 72.7886 bits (177), Expect = 1.948e-11
Identity = 32/35 (91.43%), Postives = 35/35 (100.00%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAE 405
            Q+SKIT+EGATVFPPFSLKTYWEF+AFKNPTVGAE
Sbjct:  195 QISKITDEGATVFPPFSLKTYWEFAAFKNPTVGAE 229

[Close]

BLAST of HO063888 vs. TrEMBL
Match: D7SKV3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025150001 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.690e-39
Identity = 79/90 (87.78%), Postives = 84/90 (93.33%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV G+SV+ +KYKDGILMAADMGGSYGSTLRYKSVER KPIGKHSLLGASGEISDFQEI+ YLDELIL DNMWDDGNSLGPKEV +YL
Sbjct:   25 YPYVTGTSVIGVKYKDGILMAADMGGSYGSTLRYKSVERMKPIGKHSLLGASGEISDFQEILHYLDELILYDNMWDDGNSLGPKEVHSYL 114

HSP 2 Score: 67.0106 bits (162), Expect = 1.069e-9
Identity = 28/38 (73.68%), Postives = 34/38 (89.47%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG T+  P+SLKT+W FSAF+NPTVGAEGSW
Sbjct:  208 QIAKITEEGVTISQPYSLKTFWGFSAFQNPTVGAEGSW 245

[Close]

BLAST of HO063888 vs. TrEMBL
Match: D7KB13_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474808 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.690e-39
Identity = 77/90 (85.56%), Postives = 85/90 (94.44%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV G+S+VAIKYKDG+LMA+DMGGSYGSTLRYK++ER K IGKHSLLGASGEISDFQEI+RYLDEL LNDNMWDDGNSLGPKE+ NYL
Sbjct:   26 YPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYL 115

HSP 2 Score: 66.6254 bits (161), Expect = 1.396e-9
Identity = 28/38 (73.68%), Postives = 33/38 (86.84%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG TV  P+SLKT+WEFS+F NPT GAEGSW
Sbjct:  209 QIAKITEEGVTVSQPYSLKTFWEFSSFHNPTAGAEGSW 246

[Close]

BLAST of HO063888 vs. TrEMBL
Match: B9H9B4_POPTR (Proteasome subunit beta type OS=Populus trichocarpa GN=POPTRDRAFT_561786 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.208e-39
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 2

Query:   38 RYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            +YPYV G+SVVA+KYKDGILMAADMGGSYGSTLRYKSVER KP+GKHS++GASGEISDFQEIMRYLDE +LNDNMWDD NSLGPKE+ +YL
Sbjct:   23 QYPYVTGTSVVALKYKDGILMAADMGGSYGSTLRYKSVERIKPVGKHSIIGASGEISDFQEIMRYLDEQVLNDNMWDDRNSLGPKEIHSYL 113

HSP 2 Score: 63.1586 bits (152), Expect = 1.543e-8
Identity = 25/38 (65.79%), Postives = 33/38 (86.84%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG T+  P++LKT+W + AF+NPTVGAEGSW
Sbjct:  207 QIAKITEEGVTISQPYALKTFWGYKAFENPTVGAEGSW 244

[Close]

BLAST of HO063888 vs. TrEMBL
Match: C0PPW1_PICSI (Proteasome subunit beta type OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.869e-38
Identity = 76/91 (83.52%), Postives = 85/91 (93.41%), Query Frame = 2

Query:   38 RYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            +YPYV G+SV+ IKYKDG+L+A+DMGGSYGSTLRYKSV+R KPIGKH+LLGA GEISDFQEI+RYLDELIL DNMWDDGNSLGPKEV NYL
Sbjct:   41 QYPYVTGTSVLGIKYKDGVLLASDMGGSYGSTLRYKSVQRLKPIGKHTLLGAGGEISDFQEIVRYLDELILYDNMWDDGNSLGPKEVHNYL 131

HSP 2 Score: 60.4622 bits (145), Expect = 1.000e-7
Identity = 24/38 (63.16%), Postives = 31/38 (81.58%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEGAT+  P+++KT+WE  AFKNP  GA GSW
Sbjct:  225 QIAKITEEGATISEPYAIKTHWELEAFKNPATGAVGSW 262

[Close]

BLAST of HO063888 vs. TrEMBL
Match: A9NKT0_PICSI (Proteasome subunit beta type OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.869e-38
Identity = 76/91 (83.52%), Postives = 85/91 (93.41%), Query Frame = 2

Query:   38 RYPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            +YPYV G+SV+ IKYKDG+L+A+DMGGSYGSTLRYKSV+R KPIGKH+LLGA GEISDFQEI+RYLDELIL DNMWDDGNSLGPKEV NYL
Sbjct:   41 QYPYVTGTSVLGIKYKDGVLLASDMGGSYGSTLRYKSVQRLKPIGKHTLLGAGGEISDFQEIVRYLDELILYDNMWDDGNSLGPKEVHNYL 131

HSP 2 Score: 60.4622 bits (145), Expect = 1.000e-7
Identity = 24/38 (63.16%), Postives = 31/38 (81.58%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEGAT+  P+++KT+WE  AFKNP  GA GSW
Sbjct:  225 QIAKITEEGATISEPYAIKTHWELEAFKNPATGAVGSW 262

[Close]

BLAST of HO063888 vs. TAIR peptide
Match: AT1G56450.1 (| Symbols: PBG1 | 20S proteasome beta subunit G1 | chr1:21141970-21144186 FORWARD LENGTH=246)

HSP 1 Score: 166.007 bits (419), Expect = 1.302e-41
Identity = 77/90 (85.56%), Postives = 85/90 (94.44%), Query Frame = 2

Query:   41 YPYVAGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERSKPIGKHSLLGASGEISDFQEIMRYLDELILNDNMWDDGNSLGPKEVLNYL 310
            YPYV G+S+VAIKYKDG+LMA+DMGGSYGSTLRYK++ER K IGKHSLLGASGEISDFQEI+RYLDEL LNDNMWDDGNSLGPKE+ NYL
Sbjct:   26 YPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYL 115

HSP 2 Score: 65.855 bits (159), Expect = 1.833e-11
Identity = 28/38 (73.68%), Postives = 32/38 (84.21%), Query Frame = 1

Query:  301 QLSKITEEGATVFPPFSLKTYWEFSAFKNPTVGAEGSW 414
            Q++KITEEG TV  P+SLKTYWEFS+F NPT GA GSW
Sbjct:  209 QIAKITEEGVTVSQPYSLKTYWEFSSFHNPTAGAAGSW 246

The following BLAST results are available for this feature:

BLAST of HO063888 vs. SwissProt
BLAST of HO063888 vs. TrEMBL
BLAST of HO063888 vs. TAIR peptide

BLAST of HO063888 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PSB4_ARATH	1.233e-40	85.56	Proteasome subunit beta type-4 OS=Arabidopsis thal...	[more]
PSB4_DICDI	1.015e-18	49.44	Proteasome subunit beta type-4 OS=Dictyostelium di...	[more]
PSB4_SCHPO	1.621e-16	42.72	Probable proteasome subunit beta type-4 OS=Schizos...	[more]
PSB4_XENLA	5.215e-15	34.83	Proteasome subunit beta type-4 (Fragment) OS=Xenop...	[more]
PSB4_PIG	6.811e-15	37.08	Proteasome subunit beta type-4 (Fragment) OS=Sus s...	[more]
PSB4_BOVIN	8.896e-15	37.08	Proteasome subunit beta type-4 OS=Bos taurus GN=PS...	[more]
PSB4_HUMAN	1.517e-14	35.96	Proteasome subunit beta type-4 OS=Homo sapiens GN=...	[more]
PSB4_RAT	2.588e-14	35.96	Proteasome subunit beta type-4 OS=Rattus norvegicu...	[more]
PSB4_MOUSE	3.381e-14	35.96	Proteasome subunit beta type-4 OS=Mus musculus GN=...	[more]
PSB4_YEAST	4.415e-14	44.09	Proteasome component PRE4 OS=Saccharomyces cerevis...	[more]

BLAST of HO063888 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9IKM7_POPTR	2.786e-42	81.73	Proteasome subunit beta type OS=Populus trichocarp...	[more]
B7FJ04_MEDTR	6.207e-42	86.00	Proteasome subunit beta type OS=Medicago truncatul...	[more]
C6T3I7_SOYBN	1.383e-41	94.44	Proteasome subunit beta type OS=Glycine max PE=2 S...	[more]
D7KJN2_ARALY	4.024e-41	80.58	20S proteasome beta subunit PBG1 OS=Arabidopsis ly...	[more]
C6SWQ4_SOYBN	4.024e-41	93.33	Proteasome subunit beta type OS=Glycine max PE=2 S...	[more]
D7SKV3_VITVI	1.690e-39	87.78	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KB13_ARALY	1.690e-39	85.56	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9H9B4_POPTR	2.208e-39	84.62	Proteasome subunit beta type OS=Populus trichocarp...	[more]
C0PPW1_PICSI	1.869e-38	83.52	Proteasome subunit beta type OS=Picea sitchensis P...	[more]
A9NKT0_PICSI	1.869e-38	83.52	Proteasome subunit beta type OS=Picea sitchensis P...	[more]

BLAST of HO063888 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G56450.1	1.302e-41	85.56	\| Symbols: PBG1 \| 20S proteasome beta subunit G1 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 18..82 score: 1.7
IPR016050	Proteasome, beta-type subunit, conserved site	PROSITE	PS00854	PROTEASOME_B_1	coord: 23..70 score
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 15..104 score: 1.2
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 14..103 score: 8.1
None	No IPR available	PANTHER	PTHR11599:SF5	PROTEASOME SUBUNIT BETA TYPE 4	coord: 14..103 score: 8.1
None	No IPR available	PROFILE	PS51476	PROTEASOME_B_2	coord: 19..104 score: 17
None	No IPR available	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 12..104 score: 5.6
None	No IPR available	SEG	seg	seg	coord: 1..13 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063888 ID=HO063888; Name=HO063888; organism=Cicer arietinum; type=EST; length=584bp
CGGCCGCGGGAATTCGATTTCGAGCGGCCGCCCGGGCAGGTACCCATATG
TTGCTGGCTCCTCTGTCGTTGCTATCAAATACAAAGATGGGATTCTTATG
GCTGCTGATATGGGAGGTTCATATGGATCTACGTTGCGATACAAGAGTGT
TGAGCGTTCGAAGCCTATTGGAAAGCACTCCCTTCTTGGCGCTAGTGGGG
AAATAAGCGACTTTCAGGAGATTATGCGCTACCTTGATGAGCTTATCCTT
AACGACAACATGTGGGATGATGGGAATTCTTTGGGACCTAAGGAGGTGCT
CAACTATCTAAAATTACCGAAGAAGGTGCTACTGTTTTCCCGCCATTCTC
TTTGAAAACATACTGGGAGTTCTCGGCCTTCAAGAATCCTACGGTTGGTG
CCGAAGGTTCATGGTAGCTTGCTCGTTGCGAATGAAATGATACCGAATTT
TAAATGGATTCCAACAGAATGATTCCATGTGTTGAATGGATGTGAAATGA
GTTAGGCTACTGAAGCTAAATGAGACTCAATTTGGTGTTTATTTCTTAAA
TTCTTAATGTTAGTTTGAATTGATCCAAAAAAAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004298	threonine-type endopeptidase activity
GO:0004175	endopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005839	proteasome core complex

Vocabulary: **Biological Process**
Term	Definition
GO:0051603	proteolysis involved in protein catabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001353	Proteasome_sua/b
IPR016050	Proteasome_bsu_CS

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