GR394851

EST Overview


Name	GR394851
Unique Name	GR394851
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	689

Libraries


Library Name	Type
LIBEST_024779 ICC1882 Slow drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR394851 vs. SwissProt
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 188.734 bits (478), Expect = 2.462e-47
Identity = 78/128 (60.94%), Postives = 107/128 (83.59%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +CS C+M V+W++NQL++  TKE + NY NQLCE LPSP+GES +SC+ +S+MPN++FTI +K F+LTPEQY+++  +G   VC+S F+AFDIPPP+GPLWILGDVFM AYHTVFD+G  ++GFA++A
Sbjct:  369 MCSVCEMAVVWIENQLRENKTKELILNYANQLCERLPSPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496

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BLAST of GR394851 vs. SwissProt
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 187.193 bits (474), Expect = 7.162e-47
Identity = 79/129 (61.24%), Postives = 101/129 (78.29%), Query Frame = 2

Query:   29 PLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            P+C++C+M V+W+QNQL Q  T++ + NY+NQLC+ LPSP GES + C  L+ MP ISFTIG K F L PE+Y+L+ GEG    C+S F A DIPPP+GPLWILGDVFM AYHTVFDYG ++VGFA++A
Sbjct:  381 PMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509

[Close]

BLAST of GR394851 vs. SwissProt
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.596e-46
Identity = 80/128 (62.50%), Postives = 100/128 (78.12%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +CS C+M V+W+QNQL+Q  TKER+ NY+N+LC+ +PSP G+S + C  LS MP +SFTIG K F L PE+Y+L+ GEG    C+S F AFDIPPP+GPLWILGDVFM  YHTVFD+G L+VG AEAA
Sbjct:  386 MCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513

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BLAST of GR394851 vs. SwissProt
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 4.642e-46
Identity = 79/129 (61.24%), Postives = 98/129 (75.97%), Query Frame = 2

Query:   29 PLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            P+CS+C+M V+W+QNQL Q  T++ + +YVNQLC  LPSP GES + C  L  MP+I FTIG K F L PE+Y+L+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFDYG L++GFA+AA
Sbjct:  380 PMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

[Close]

BLAST of GR394851 vs. SwissProt
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 183.341 bits (464), Expect = 1.034e-45
Identity = 81/127 (63.78%), Postives = 98/127 (77.17%), Query Frame = 2

Query:   35 CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            C  C   V+W+QNQ++Q  T+E + NYV++LCE LPSP GES + C+ LS MPNI+FT+G K F L+PEQYVL+ GEG T  C+S F A D+ PP GPLWILGDVFM  YHTVFDYGNL+VGFAEAA
Sbjct:  347 CVPCARWVVWMQNQIRQNETEENIINYVDKLCERLPSPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473

[Close]

BLAST of GR394851 vs. SwissProt
Match: CARDA_CYNCA (Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 3.806e-40
Identity = 76/128 (59.38%), Postives = 94/128 (73.44%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +C+ C+M V+W+QN++KQ  T++ + NY N+LCE L + S E  + CN LS MPN+SFTIG K F LTPEQY+L+ G+G    C+S F A D     GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  378 MCTFCEMAVVWMQNEIKQSETEDNIINYANELCEHLSTSSEELQVDCNTLSSMPNVSFTIGGKKFGLTPEQYILKVGKGEATQCISGFTAMD-ATLLGPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 504

[Close]

BLAST of GR394851 vs. SwissProt
Match: CARDB_CYNCA (Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 8.480e-40
Identity = 68/127 (53.54%), Postives = 97/127 (76.38%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            +C+ C+M ++ +QN++K+  T++ + N+VN++C+ LP+ S ES++ CN +S MPNI+FTIG K F +TPEQY+ + GEG    C+S F A DI  P+GP+WILGD+FM  YHTVFDYG L+VGFAEA
Sbjct:  379 ICTFCEMALVRMQNEIKRNETEDNIINHVNEVCDQLPTSSAESIVDCNGISSMPNIAFTIGSKLFEVTPEQYIYKVGEGEAATCISGFTALDIMSPQGPIWILGDMFMGPYHTVFDYGKLRVGFAEA 505

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BLAST of GR394851 vs. SwissProt
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.030e-21
Identity = 47/94 (50.00%), Postives = 63/94 (67.02%), Query Frame = 2

Query:  134 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            ++P   GE +I C  +S +P I+  +G K + L+PE Y L+  +    +CLS F+  DIPPP GPLWILGDVF+  Y+TVFD  N +VGFAEAA
Sbjct:  317 AVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410

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BLAST of GR394851 vs. SwissProt
Match: CATD_BOVIN (Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 3.957e-21
Identity = 46/94 (48.94%), Postives = 62/94 (65.96%), Query Frame = 2

Query:  134 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            ++P   GE +I C  +S +P ++  +G K + L+PE Y L+  +  T VCLS F+  DIPPP GPLWILGDVF+  Y+TVFD    +VG AEAA
Sbjct:  295 AVPLIQGEYMIPCEKVSSLPEVTVKLGGKDYALSPEDYALKVSQAETTVCLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDQNRVGLAEAA 388

[Close]

BLAST of GR394851 vs. SwissProt
Match: CATD_MOUSE (Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 6.750e-21
Identity = 44/93 (47.31%), Postives = 63/93 (67.74%), Query Frame = 2

Query:  134 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            ++P   GE +I C  +S +P +   +G K + L P++Y+L+  +G   +CLS F+  DIPPP GPLWILGDVF+ +Y+TVFD  N +VGFA A
Sbjct:  315 AVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFANA 407

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q9SXU0_CICAR (Aspartic proteinase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.195e-75
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:   67 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 204

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q2HRQ7_MEDTR (Peptidase A1, pepsin OS=Medicago truncatula GN=MtrDRAFT_AC157894g38v2 PE=3 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 6.610e-72
Identity = 128/138 (92.75%), Postives = 136/138 (98.55%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            QSE+SA DTPLCSSCQMLV+WVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCND+S+MPNISFTIG+KPFVLTPEQY+LRTGEGIT+VCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:  259 QSELSAKDTPLCSSCQMLVLWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDISKMPNISFTIGNKPFVLTPEQYILRTGEGITQVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 396

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q948P0_SOYBN (Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 1.055e-69
Identity = 123/137 (89.78%), Postives = 133/137 (97.08%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            Q E++A DTPLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+LRTGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:  371 QEELAARDTPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 507

[Close]

BLAST of GR394851 vs. TrEMBL
Match: C6TIU2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 1.055e-69
Identity = 123/137 (89.78%), Postives = 133/137 (97.08%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            Q E++A DTPLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+LRTGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:    7 QEELAARDTPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 143

[Close]

BLAST of GR394851 vs. TrEMBL
Match: C6TDL8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 8.934e-69
Identity = 121/137 (88.32%), Postives = 132/137 (96.35%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            Q E++A D PLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+L+TGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:   40 QGELTARDNPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILKTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 176

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q8H948_SOYBN (Aspartic proteinase 5 (Fragment) OS=Glycine max GN=soyAP5 PE=2 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 2.131e-62
Identity = 109/138 (78.99%), Postives = 124/138 (89.86%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            Q E+S  DT LC+SCQMLV+W+QNQLKQK TKE VFNYVNQLCESLPSP+GESV+ CN +  +PNI+FT+GDKPF LTPEQY+L+TGEGI EVCLS FIAFDIPPP+GPLWILGDVFMR YHTVFDYGNL+VGFA+AA
Sbjct:   38 QRELSTKDTALCTSCQMLVVWIQNQLKQKKTKEIVFNYVNQLCESLPSPNGESVVDCNSIYGLPNITFTVGDKPFTLTPEQYILKTGEGIAEVCLSGFIAFDIPPPRGPLWILGDVFMRVYHTVFDYGNLRVGFAKAA 175

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q93XR0_IPOBA (Putative aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 6.007e-57
Identity = 100/138 (72.46%), Postives = 118/138 (85.51%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            ++E + T  PLC++C+M VIW+QNQLKQK  KE+VF YV+QLCE LPSP GESVI CN +S MPN++F IGDK FVLTPEQY+L+TGEGI  VC+S F+A D+PPP+GPLWILGDVFM AYHTVFDYGNLQVGFAEAA
Sbjct:  367 EAEGNLTSNPLCTTCEMAVIWLQNQLKQKGIKEKVFEYVDQLCEKLPSPDGESVIDCNSISSMPNVTFVIGDKDFVLTPEQYILKTGEGIAAVCVSGFLALDVPPPQGPLWILGDVFMGAYHTVFDYGNLQVGFAEAA 504

[Close]

BLAST of GR394851 vs. TrEMBL
Match: B9I6C6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834438 PE=3 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 4.305e-55
Identity = 97/138 (70.29%), Postives = 118/138 (85.51%), Query Frame = 2

Query:    2 QSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +   +  D P C++CQMLVIWVQNQL++KATKE   NY+++LCESLPSP G+S I CN +S MPNI+FTIGDKPF LTPEQY+L+TGEGI +VC+S F+A D+PPP+GPLWILGDVFM AYHT+FDYGNL+VGFAEAA
Sbjct:  371 EKSSAGNDLP-CTACQMLVIWVQNQLREKATKETAINYLDKLCESLPSPMGQSSIDCNSISTMPNITFTIGDKPFSLTPEQYILKTGEGIAQVCISGFMALDVPPPRGPLWILGDVFMGAYHTIFDYGNLEVGFAEAA 507

[Close]

BLAST of GR394851 vs. TrEMBL
Match: B1Q478_CAPCH (Putative aspartic protease OS=Capsicum chinense PE=2 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 2.136e-54
Identity = 95/134 (70.90%), Postives = 112/134 (83.58%), Query Frame = 2

Query:   14 SATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            S  + PLC+ C+M V+W+QNQLKQ+ TKERV  YV+QLCE LPSP GESV+ CN +S +PNI+FTI DK FVLTPEQY+L+TGEGI  +C+S F AFD+PPP+GPLWILGDVFM  YHTVFDYGN QVGFAEAA
Sbjct:  373 SVGEAPLCTVCEMAVVWIQNQLKQQGTKERVLEYVDQLCEKLPSPMGESVVDCNSISSLPNITFTIKDKAFVLTPEQYILKTGEGIASICISGFAAFDVPPPRGPLWILGDVFMGPYHTVFDYGNSQVGFAEAA 506

[Close]

BLAST of GR394851 vs. TrEMBL
Match: Q06EY4_IPOBA (Aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.385e-53
Identity = 90/137 (65.69%), Postives = 114/137 (83.21%), Query Frame = 2

Query:    5 SEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +E  ++  P+C++C+M V+W+QNQLKQK  KE+VF+YVNQLCE +PSP GES I CN +S MPN++F I DK FVLTPEQY+L+TGEG+  +C+S F+A D+P P+GPLWILGDVFM  YHTVFDYGNLQ+GFAEAA
Sbjct:  372 NEGKSSSDPMCTACEMAVVWMQNQLKQKVVKEKVFDYVNQLCEKIPSPMGESTIDCNSISNMPNVTFKIADKDFVLTPEQYILKTGEGVATICVSGFLAMDVPAPRGPLWILGDVFMGVYHTVFDYGNLQIGFAEAA 508

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT1G62290.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 186.037 bits (471), Expect = 1.610e-47
Identity = 79/126 (62.70%), Postives = 101/126 (80.16%), Query Frame = 2

Query:   35 CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            C +C+M V+W+Q+QL+Q  T+ER+ NY+N++CE +PSP+GES + C+ LS+MP +SFTIG K F L PE+YVL+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  387 CPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT1G62290.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 186.037 bits (471), Expect = 1.610e-47
Identity = 79/126 (62.70%), Postives = 101/126 (80.16%), Query Frame = 2

Query:   35 CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 412
            C +C+M V+W+Q+QL+Q  T+ER+ NY+N++CE +PSP+GES + C+ LS+MP +SFTIG K F L PE+YVL+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  387 CPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT1G11910.1 (| Symbols: APA1, ATAPA1 | aspartic proteinase A1 | chr1:4017119-4019874 REVERSE LENGTH=506)

HSP 1 Score: 185.267 bits (469), Expect = 2.747e-47
Identity = 82/127 (64.57%), Postives = 99/127 (77.95%), Query Frame = 2

Query:   35 CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            CS+C+M V+W+Q+QL+Q  T+ER+ NYVN+LCE LPSP GES + C  LS MP +S TIG K F L PE+YVL+ GEG    C+S FIA D+ PP+GPLWILGDVFM  YHTVFD+GN QVGFAEAA
Sbjct:  380 CSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT4G04460.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=504)

HSP 1 Score: 152.14 bits (383), Expect = 2.577e-37
Identity = 62/128 (48.44%), Postives = 92/128 (71.88%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +CS+C+M  +W++++L Q  T+ER+  Y  +LC+ +P+ + +S + C  +S MP ++F+IG + F LTP+ Y+ + GEG+   C S F A DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  377 MCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT4G04460.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=508)

HSP 1 Score: 152.14 bits (383), Expect = 2.577e-37
Identity = 62/128 (48.44%), Postives = 92/128 (71.88%), Query Frame = 2

Query:   32 LCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 415
            +CS+C+M  +W++++L Q  T+ER+  Y  +LC+ +P+ + +S + C  +S MP ++F+IG + F LTP+ Y+ + GEG+   C S F A DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  381 MCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

[Close]

BLAST of GR394851 vs. TAIR peptide
Match: AT4G22050.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr4:11683865-11685506 FORWARD LENGTH=354)

HSP 1 Score: 63.929 bits (154), Expect = 9.229e-11
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 2

Query:  167 SCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGN---LQVGFAEA 412
            +CN+   +P+++FTIG K FVLTP  Y+ R+       C S F+           W LG  FMR +HTVFDY N   ++VGFA++
Sbjct:  276 NCNNFETLPDVTFTIGGKAFVLTPLDYIRRS----RSQCTSKFVG----KTNRSHWTLGIPFMRVFHTVFDYQNTLAVKVGFAKS 352

The following BLAST results are available for this feature:

BLAST of GR394851 vs. SwissProt
BLAST of GR394851 vs. TrEMBL
BLAST of GR394851 vs. TAIR peptide

BLAST of GR394851 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ASPRX_ORYSJ	2.462e-47	60.94	Aspartic proteinase OS=Oryza sativa subsp. japonic...	[more]
ASPR1_ORYSJ	7.162e-47	61.24	Aspartic proteinase oryzasin-1 OS=Oryza sativa sub...	[more]
ASPR_CUCPE	1.596e-46	62.50	Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1	[more]
ASPR_HORVU	4.642e-46	61.24	Phytepsin OS=Hordeum vulgare PE=1 SV=1	[more]
CYPR1_CYNCA	1.034e-45	63.78	Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO...	[more]
CARDA_CYNCA	3.806e-40	59.38	Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 ...	[more]
CARDB_CYNCA	8.480e-40	53.54	Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 ...	[more]
CATD_HUMAN	3.030e-21	50.00	Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1	[more]
CATD_BOVIN	3.957e-21	48.94	Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2	[more]
CATD_MOUSE	6.750e-21	47.31	Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1	[more]

BLAST of GR394851 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SXU0_CICAR	2.195e-75	100.00	Aspartic proteinase (Fragment) OS=Cicer arietinum ...	[more]
Q2HRQ7_MEDTR	6.610e-72	92.75	Peptidase A1, pepsin OS=Medicago truncatula GN=Mtr...	[more]
Q948P0_SOYBN	1.055e-69	89.78	Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=...	[more]
C6TIU2_SOYBN	1.055e-69	89.78	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TDL8_SOYBN	8.934e-69	88.32	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8H948_SOYBN	2.131e-62	78.99	Aspartic proteinase 5 (Fragment) OS=Glycine max GN...	[more]
Q93XR0_IPOBA	6.007e-57	72.46	Putative aspartic protease OS=Ipomoea batatas PE=2...	[more]
B9I6C6_POPTR	4.305e-55	70.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B1Q478_CAPCH	2.136e-54	70.90	Putative aspartic protease OS=Capsicum chinense PE...	[more]
Q06EY4_IPOBA	1.385e-53	65.69	Aspartic protease OS=Ipomoea batatas PE=2 SV=1	[more]

BLAST of GR394851 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G62290.2	1.610e-47	62.70	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT1G62290.1	1.610e-47	62.70	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT1G11910.1	2.747e-47	64.57	\| Symbols: APA1, ATAPA1 \| aspartic proteinase A1 \|...	[more]
AT4G04460.2	2.577e-37	48.44	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G04460.1	2.577e-37	48.44	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G22050.1	9.229e-11	41.18	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Peptidase A1	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 30..137 score: 2.8
IPR001461	Peptidase A1	PFAM	PF00026	Asp	coord: 30..137 score: 1.5
IPR007856	Saposin-like type B, 1	PFAM	PF05184	SapB_1	coord: 10..47 score: 1
IPR008139	Saposin B	PROFILE	PS50015	SAP_B	coord: 8..49 score: 12
IPR009007	Peptidase aspartic, catalytic	GENE3D	2.40.70.10	no description	coord: 42..138 score: 1.7
IPR011001	Saposin-like	GENE3D	1.10.225.10	no description	coord: 8..41 score: 7.1
IPR011001	Saposin-like	SUPERFAMILY	47862	Saposin	coord: 4..47 score: 4.5
IPR021109	Peptidase aspartic	SUPERFAMILY	50630	Acid proteases	coord: 33..138 score: 2.5
None	No IPR available	PANTHER	PTHR13683:SF83	ASPARTIC PROTEINASE-RELATED	coord: 30..137 score: 2.8
None	No IPR available	SEG	seg	seg	coord: 27..39 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR394851 ID=GR394851; Name=GR394851; organism=Cicer arietinum; type=EST; length=689bp
ACAGAGTGAGATGTCAGCTACAGATACTCCTTTGTGTTCTTCTTGTCAGA
TGCTTGTTATTTGGGTCCAGAATCAACTAAAACAAAAGGCGACCAAGGAA
AGAGTATTCAATTATGTGAATCAACTGTGTGAGAGTCTTCCAAGTCCATC
CGGAGAATCAGTGATAAGCTGTAACGATCTTTCTCGGATGCCAAACATAT
CATTCACTATCGGAGACAAACCCTTCGTCCTCACACCAGAACAGTATGTT
CTAAGAACTGGAGAAGGCATTACAGAGGTCTGCCTTAGTGCGTTTATTGC
TTTTGATATTCCTCCTCCAAAGGGTCCACTCTGGATTCTTGGTGATGTTT
TCATGAGGGCATATCACACAGTCTTCGACTACGGAAACCTTCAAGTTGGT
TTTGCAGAAGCTGCATAGTTACCACATACCACTTATGGAGTAATATCTTA
AGGAATATTTTTCATAATTTCGTTTATGCATTGTAAATAAACTGTTTGTG
CTACTATGTTACCTACTATACCCTTAGGTTCACTTCATGTATTCCCATAT
ATATGTATTCCCGGAGGTATTTATTTATTCTATCCCCCTTATGATACCAG
CAGGACTAATATTCCCCATTATAAAAAAATTTTTACTTATTCTAACTGCT
TCTCCTGTTTATTATACCCCTTGCTTAAATACCTAAAGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis
GO:0006629	lipid metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR007856	SapB_1
IPR008139	SaposinB_dom
IPR009007	Peptidase aspartic, catalytic
IPR011001	Saposin-like
IPR021109	Peptidase_aspartic_dom_sf

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