GR915296

EST Overview


Name	GR915296
Unique Name	GR915296
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	282

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC1_PEA (Pyruvate decarboxylase isozyme 1 OS=Pisum sativum GN=PDC1 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.267e-18
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ
Sbjct:  552 TGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 593

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC2_PEA (Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pisum sativum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.940e-15
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  364 TGPKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 405

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC1_MAIZE (Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PDC1 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.940e-15
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  569 TGAKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 610

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC1_ORYSJ (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. japonica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.840e-15
Identity = 35/42 (83.33%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIE+  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  564 TGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPPNPQ 605

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC1_ORYSI (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. indica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.840e-15
Identity = 35/42 (83.33%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIE+  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  564 TGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPPNPQ 605

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC2_ORYSI (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. indica GN=PDC2 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 5.015e-15
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 1

Query:   67 GAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            G +KD LCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  566 GEKKDCLCFIEVIAHKDDTSKELLEWGSRVSAANSRPPNPQ 606

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC3_MAIZE (Pyruvate decarboxylase isozyme 3 (Fragment) OS=Zea mays GN=PDC3 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.550e-15
Identity = 36/39 (92.31%), Postives = 37/39 (94.87%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            EKD LCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  164 EKDCLCFIEVIAHKDDTSKELLEWGSRVSAANSRPPNPQ 202

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC2_ORYSJ (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. japonica GN=PDC2 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.550e-15
Identity = 36/39 (92.31%), Postives = 37/39 (94.87%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            EKD LCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  567 EKDCLCFIEVIAHKDDTSKELLEWGSRVSAANSRPPNPQ 605

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC3_ORYSJ (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. japonica GN=PDC3 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 8.555e-15
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G + DSLCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  546 TGDKADSLCFIEVVAHKDDTSKELLEWGSRVSAANSRPPNPQ 587

[Close]

BLAST of GR915296 vs. SwissProt
Match: PDC3_ORYSI (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. indica GN=PDC3 PE=2 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 8.555e-15
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G + DSLCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  546 TGDKADSLCFIEVVAHKDDTSKELLEWGSRVSAANSRPPNPQ 587

[Close]

BLAST of GR915296 vs. TrEMBL
Match: Q4JIY7_CITSI (Pyruvate decarboxylase (Fragment) OS=Citrus sinensis PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.416e-15
Identity = 37/42 (88.10%), Postives = 40/42 (95.24%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIEVF HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  158 TGEQKDSLCFIEVFVHKDDTSKELLEWGSRVSAANSRPPNPQ 199

[Close]

BLAST of GR915296 vs. TrEMBL
Match: Q1I1D9_CITSI (Pyruvate decarboxylase OS=Citrus sinensis PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.416e-15
Identity = 37/42 (88.10%), Postives = 40/42 (95.24%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIEVF HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  548 TGEQKDSLCFIEVFVHKDDTSKELLEWGSRVSAANSRPPNPQ 589

[Close]

BLAST of GR915296 vs. TrEMBL
Match: Q684K0_LOTJA (Pyruvate decarboxylase isozyme 1 OS=Lotus japonicus GN=pdc1 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.230e-14
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIEVF HKDDTSKELLEWGSRVAAAN RPPNPQ
Sbjct:  539 TGPQKDSLCFIEVFVHKDDTSKELLEWGSRVAAANGRPPNPQ 580

[Close]

BLAST of GR915296 vs. TrEMBL
Match: D7TJI9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021184001 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.230e-14
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA KDSLCFIEVF HKDDTSKELLEWGSRV+AAN RPPNPQ
Sbjct:  536 TGAHKDSLCFIEVFVHKDDTSKELLEWGSRVSAANGRPPNPQ 577

[Close]

BLAST of GR915296 vs. TrEMBL
Match: B9IGD0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_835585 PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.230e-14
Identity = 37/42 (88.10%), Postives = 41/42 (97.62%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G++KDSLCFIEV AHKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  564 TGSKKDSLCFIEVIAHKDDTSKELLEWGSRVSAANSRPPNPQ 605

[Close]

BLAST of GR915296 vs. TrEMBL
Match: B9H2K8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_817822 PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.230e-14
Identity = 36/42 (85.71%), Postives = 40/42 (95.24%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIE+F HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  541 TGEQKDSLCFIEIFVHKDDTSKELLEWGSRVSAANSRPPNPQ 582

[Close]

BLAST of GR915296 vs. TrEMBL
Match: B9I1N8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1094038 PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.740e-14
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +G +KDSLCFIEVF HKDDTSKELLEWGSRV+AAN RPPNPQ
Sbjct:  552 TGEQKDSLCFIEVFVHKDDTSKELLEWGSRVSAANGRPPNPQ 593

[Close]

BLAST of GR915296 vs. TrEMBL
Match: Q00KE1_COILA (Pyruvate decarboxylase (Fragment) OS=Coix lachryma-jobi PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.103e-14
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  121 TGAKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 162

[Close]

BLAST of GR915296 vs. TrEMBL
Match: C5YZE5_SORBI (Putative uncharacterized protein Sb09g023060 OS=Sorghum bicolor GN=Sb09g023060 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.103e-14
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  568 TGAKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 609

[Close]

BLAST of GR915296 vs. TrEMBL
Match: C4J495_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.103e-14
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 1

Query:   64 SGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +GA+KD LCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  568 TGAKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 609

[Close]

BLAST of GR915296 vs. TAIR peptide
Match: AT5G54960.1 (| Symbols: PDC2 | pyruvate decarboxylase-2 | chr5:22310858-22312681 REVERSE LENGTH=607)

HSP 1 Score: 76.6406 bits (187), Expect = 3.333e-15
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            EK+S CFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  569 EKESFCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ 607

[Close]

BLAST of GR915296 vs. TAIR peptide
Match: AT5G01330.1 (| Symbols: PDC3 | pyruvate decarboxylase-3 | chr5:132538-134807 REVERSE LENGTH=592)

HSP 1 Score: 76.6406 bits (187), Expect = 3.333e-15
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +KDSLCFIEV  HKDDTSKELLEWGSRV+AAN RPPNPQ
Sbjct:  554 KKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ 592

[Close]

BLAST of GR915296 vs. TAIR peptide
Match: AT5G01320.1 (| Symbols: | Thiamine pyrophosphate dependent pyruvate decarboxylase family protein | chr5:129484-131625 REVERSE LENGTH=603)

HSP 1 Score: 76.6406 bits (187), Expect = 3.333e-15
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +KDSLCFIEV  HKDDTSKELLEWGSRV+AAN RPPNPQ
Sbjct:  565 KKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ 603

[Close]

BLAST of GR915296 vs. TAIR peptide
Match: AT4G33070.1 (| Symbols: | Thiamine pyrophosphate dependent pyruvate decarboxylase family protein | chr4:15952519-15954676 REVERSE LENGTH=607)

HSP 1 Score: 75.8702 bits (185), Expect = 5.685e-15
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1

Query:   73 EKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 189
            +KD LCFIEV  HKDDTSKELLEWGSRV+AANSRPPNPQ
Sbjct:  569 KKDCLCFIEVILHKDDTSKELLEWGSRVSAANSRPPNPQ 607

The following BLAST results are available for this feature:

BLAST of GR915296 vs. SwissProt
BLAST of GR915296 vs. TrEMBL
BLAST of GR915296 vs. TAIR peptide

BLAST of GR915296 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PDC1_PEA	8.267e-18	97.62	Pyruvate decarboxylase isozyme 1 OS=Pisum sativum ...	[more]
PDC2_PEA	2.940e-15	85.71	Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pis...	[more]
PDC1_MAIZE	2.940e-15	85.71	Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PD...	[more]
PDC1_ORYSJ	3.840e-15	83.33	Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s...	[more]
PDC1_ORYSI	3.840e-15	83.33	Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s...	[more]
PDC2_ORYSI	5.015e-15	87.80	Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s...	[more]
PDC3_MAIZE	6.550e-15	92.31	Pyruvate decarboxylase isozyme 3 (Fragment) OS=Zea...	[more]
PDC2_ORYSJ	6.550e-15	92.31	Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s...	[more]
PDC3_ORYSJ	8.555e-15	85.71	Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s...	[more]
PDC3_ORYSI	8.555e-15	85.71	Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s...	[more]

BLAST of GR915296 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q4JIY7_CITSI	9.416e-15	88.10	Pyruvate decarboxylase (Fragment) OS=Citrus sinens...	[more]
Q1I1D9_CITSI	9.416e-15	88.10	Pyruvate decarboxylase OS=Citrus sinensis PE=2 SV=...	[more]
Q684K0_LOTJA	1.230e-14	88.10	Pyruvate decarboxylase isozyme 1 OS=Lotus japonicu...	[more]
D7TJI9_VITVI	1.230e-14	88.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9IGD0_POPTR	1.230e-14	88.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H2K8_POPTR	1.230e-14	85.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I1N8_POPTR	2.740e-14	85.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q00KE1_COILA	6.103e-14	85.71	Pyruvate decarboxylase (Fragment) OS=Coix lachryma...	[more]
C5YZE5_SORBI	6.103e-14	85.71	Putative uncharacterized protein Sb09g023060 OS=So...	[more]
C4J495_MAIZE	6.103e-14	85.71	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GR915296 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G54960.1	3.333e-15	87.18	\| Symbols: PDC2 \| pyruvate decarboxylase-2 \| chr5:...	[more]
AT5G01330.1	3.333e-15	87.18	\| Symbols: PDC3 \| pyruvate decarboxylase-3 \| chr5:...	[more]
AT5G01320.1	3.333e-15	87.18	\| Symbols: \| Thiamine pyrophosphate dependent pyr...	[more]
AT4G33070.1	5.685e-15	87.18	\| Symbols: \| Thiamine pyrophosphate dependent pyr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.970	no description	coord: 6..60 score: 0.
None	No IPR available	PANTHER	PTHR18968	THIAMINE PYROPHOSPHATE ENZYMES	coord: 7..63 score: 2.4
None	No IPR available	PANTHER	PTHR18968:SF4	PYRUVATE DECARBOXYLASE	coord: 7..63 score: 2.4
None	No IPR available	SEG	seg	seg	coord: 8..21 score
None	No IPR available	SEG	seg	seg	coord: 44..68 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR915296 ID=GR915296; Name=GR915296; organism=Cicer arietinum; type=EST; length=282bp
GATTTCGAGGCGGCCGCCCGGGCAGGTACAGAGGAAGAGCTGATAGAAGC
AATAGCAACAGCTAGTGGAGCAGAGAAAGATTCACTATGTTTTATAGAAG
TTTTTGCACACAAGGATGACACCAGCAAAGAGTTGCTAGAATGGGGATCT
CGTGTTGCTGCTGCTAACAGCCGTCCTCCAAATCCCCAATAGAAAATAAA
TTCCTAATTATTGCATATCAAAAGAATTATTACAATATGTATATTTTTGT
GTGCTTACTTGGAATAAATGATCATTTTTCTG

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