HO062767

EST Overview


Name	HO062767
Unique Name	HO062767
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	606

Libraries


Library Name	Type
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS3_ARATH (Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.429e-15
Identity = 29/40 (72.50%), Postives = 37/40 (92.50%), Query Frame = -2

Query:  243 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 362
            GA+L++RSA+G RAFLYPIV+P CDL+GFEVLSI+HP D+
Sbjct:  226 GAVLMLRSAHGPRAFLYPIVEP-CDLQGFEVLSIYHPTDD 264

HSP 2 Score: 38.891 bits (89), Expect = 1.429e-15
Identity = 31/79 (39.24%), Postives = 44/79 (55.70%), Query Frame = -1

Query:  367 MFHNYDIDPL--XIQSL*FISSTPRLYQKXXFSX**TFFLCEKMFLKIYNVVFLGLHLVWHG*KGKKQKVINHLAKYMA 597
            +FHN+DIDP    + SL  +SS P + Q+  F       + E   LK ++VVFL   +  +  K +K KVI HL K+MA
Sbjct:  151 IFHNFDIDPSANSLASL-LVSSDPDISQRMFFHTVDIMDVTES--LKSFDVVFLAALVGMN--KEEKVKVIEHLQKHMA 224

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS_SOLLC (Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 7.250e-14
Identity = 34/61 (55.74%), Postives = 44/61 (72.13%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+ K K  +         GA L++RSA+GARAFLYP++DP  DL+GFEVLS++HP DE
Sbjct:  204 GMDKEDKVKV-VDHLAKYMSPGATLMLRSAHGARAFLYPVLDP-RDLRGFEVLSVYHPTDE 262

HSP 2 Score: 35.8094 bits (81), Expect = 7.250e-14
Identity = 22/56 (39.29%), Postives = 30/56 (53.57%), Query Frame = -3

Query:  104 VINSVMVARKCSVNQEVYL----------KSKCCEVEGFINPLNHGNVIEELSIDE 241
            VINSV++ARK  V     L          K  C E+  F NPLN  N++EE +++E
Sbjct:  263 VINSVIIARKLPVPSVPLLDGLGAYVLPSKCACAEIHAF-NPLNKMNLVEEFALEE 317

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS4_ARATH (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.441e-10
Identity = 36/61 (59.02%), Postives = 45/61 (73.77%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDKK K K  +         GA+L++RSA+G RAFLYPIV+P CDL+GFEVLS++HP DE
Sbjct:  207 GMDKKEKVKV-VEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP-CDLEGFEVLSVYHPTDE 265

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS2_ARATH (Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.343e-10
Identity = 34/61 (55.74%), Postives = 44/61 (72.13%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+ K K  +         GA++++RSA+G RAFLYPIVD  CDLKGFEVL+I+HP D+
Sbjct:  206 GMDKESKVKA-IEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD-SCDLKGFEVLTIYHPSDD 264

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS1_ARATH (Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.142e-7
Identity = 31/61 (50.82%), Postives = 42/61 (68.85%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+ K K  +         GA+L++RSA+  RAFLYPIVD   DLKGF++L+I+HP D+
Sbjct:  207 GMDKESKVKA-IEHLEKHMAPGAVLMLRSAHALRAFLYPIVD-SSDLKGFQLLTIYHPTDD 265

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS1_ORYSJ (Nicotianamine synthase 1 OS=Oryza sativa subsp. japonica GN=NAS1 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.414e-7
Identity = 29/43 (67.44%), Postives = 35/43 (81.40%), Query Frame = -2

Query:  243 DGAILVVRSAYGARAFLYPIVDPCCDLK--GFEVLSIFHPIDE 365
            DGA LVVRSA+GAR FLYPIVDP  D++  GF+VL++ HP DE
Sbjct:  227 DGAALVVRSAHGARGFLYPIVDP-EDVRRGGFDVLAVCHPEDE 268

HSP 2 Score: 20.4014 bits (41), Expect = 3.414e-7
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = -3

Query:  212 VINSVMVARK 241
            VINSV+VARK
Sbjct:  269 VINSVIVARK 278

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS1_ORYSI (Nicotianamine synthase 1 OS=Oryza sativa subsp. indica GN=NAS1 PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 3.414e-7
Identity = 29/43 (67.44%), Postives = 35/43 (81.40%), Query Frame = -2

Query:  243 DGAILVVRSAYGARAFLYPIVDPCCDLK--GFEVLSIFHPIDE 365
            DGA LVVRSA+GAR FLYPIVDP  D++  GF+VL++ HP DE
Sbjct:  227 DGAALVVRSAHGARGFLYPIVDP-EDVRRGGFDVLAVCHPEDE 268

HSP 2 Score: 20.4014 bits (41), Expect = 3.414e-7
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = -3

Query:  212 VINSVMVARK 241
            VINSV+VARK
Sbjct:  269 VINSVIVARK 278

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS2_ORYSJ (Nicotianamine synthase 2 OS=Oryza sativa subsp. japonica GN=NAS2 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.426e-7
Identity = 28/43 (65.12%), Postives = 35/43 (81.40%), Query Frame = -2

Query:  243 DGAILVVRSAYGARAFLYPIVDPCCDLK--GFEVLSIFHPIDE 365
            DGA LVVRSA+GAR FLYPIVD   D++  GF+VL+++HP DE
Sbjct:  227 DGAALVVRSAHGARGFLYPIVD-LEDIRRGGFDVLAVYHPDDE 268

HSP 2 Score: 20.7866 bits (42), Expect = 4.426e-7
Identity = 14/39 (35.90%), Postives = 18/39 (46.15%), Query Frame = -3

Query:  161 VINSVMVARKCSVNQ------------EVYLKSKCCEVE 241
            VINSV+VARK    +             V    KCC++E
Sbjct:  269 VINSVIVARKADPRRGGGLAGARGAVPVVSPPCKCCKME 307

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS3_HORVU (Probable nicotianamine synthase 3 OS=Hordeum vulgare GN=NAS3 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 7.403e-7
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = -2

Query:  243 DGAILVVRSAYGARAFLYPIVDP-CCDLKGFEVLSIFHPIDE 365
            DGA LVVRSA+GAR FLYPIVDP      GFEVL++ HP D+
Sbjct:  228 DGAALVVRSAHGARGFLYPIVDPQDIGRGGFEVLAVCHPDDD 269

[Close]

BLAST of HO062767 vs. SwissProt
Match: NAS2_HORVU (Probable nicotianamine synthase 2 OS=Hordeum vulgare GN=NAS2 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 7.403e-7
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = -2

Query:  243 DGAILVVRSAYGARAFLYPIVDP-CCDLKGFEVLSIFHPIDE 365
            DGA LVVRSA+GAR FLYPIVDP      GFEVL++ HP D+
Sbjct:  228 DGAALVVRSAHGARGFLYPIVDPQDIGRGGFEVLAVCHPDDD 269

[Close]

BLAST of HO062767 vs. TrEMBL
Match: A3DUI9_MALXI (Nicotianamine synthase OS=Malus xiaojinensis PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 7.132e-23
Identity = 30/40 (75.00%), Postives = 37/40 (92.50%), Query Frame = -2

Query:  243 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 362
            GA L++RSA+GARAFLYP++DPC DL+GFEVLS+FHP DE
Sbjct:  223 GATLMLRSAHGARAFLYPVIDPCDDLRGFEVLSVFHPTDE 262

HSP 2 Score: 42.743 bits (99), Expect = 7.132e-23
Identity = 25/62 (40.32%), Postives = 38/62 (61.29%), Query Frame = -3

Query:  101 VINSVMVARKCSVNQE--------------VYLKSKCCEVEGFINPLN-HGNVIEELSIDEK 241
            VINS+++ARK ++  +              + L SKCCE+E F NPL+ HGN+IEE + +E+
Sbjct:  263 VINSIVIARKYALPVQHSSPIDSQGLNAGAIILPSKCCELEAF-NPLHKHGNMIEEFAFEEQ 323

HSP 3 Score: 37.7354 bits (86), Expect = 7.132e-23
Identity = 30/81 (37.04%), Postives = 42/81 (51.85%), Query Frame = -1

Query:  367 TTNMFHNYDIDP-LXIQSL*FISSTPRLYQKXXFSX**TFFLCEKMFLKIYNVVFLGLHLVWHG*KGKKQKVINHLAKYMA 606
            T   F NYDI+     ++L  +SS P L ++  F    T  +     LK Y +VFL   +     K +K K+I+HLAKYMA
Sbjct:  145 TKTSFDNYDINASANSKALGLVSSDPDLSKRMVFHT--TDIMDVTTALKDYEIVFLAALVGMD--KMEKLKIIDHLAKYMA 221

[Close]

BLAST of HO062767 vs. TrEMBL
Match: B9A9Z2_LOTJA (Nicotianamine synthase OS=Lotus japonicus GN=LjNAS2 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 9.440e-23
Identity = 51/106 (48.11%), Postives = 62/106 (58.49%), Query Frame = -2

Query:  243 KAYDLFHPHQDFIKRXXFXTNKHFFYVKKCS*RFIMLCFWACIWFGMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 560
            KAYDL     D  KR  F T     +V      + ++   A +  GMDKKGK    +         GA+LV+RSA+GARAFLYP+VDP  DLKGFEVLS+FHP DE
Sbjct:  161 KAYDLVSSDPDLSKRMFFHTTD-IAHVSNALKEYNVVFLAALV--GMDKKGKESV-INHLAKHMAPGAVLVLRSAHGARAFLYPVVDP-SDLKGFEVLSVFHPTDE 261

HSP 2 Score: 51.6026 bits (122), Expect = 9.440e-23
Identity = 34/52 (65.38%), Postives = 38/52 (73.08%), Query Frame = -3

Query:   98 VINSVMVARKCSVNQEVYLKSKCCEV--EGFINPLNHGNVIEELS--IDEKA 241
            VINSV+VARK SVN  + L SKC E+  EGF N LNH NVI+ELS  IDE A
Sbjct:  262 VINSVIVARKHSVNPGILLSSKCHELAAEGF-NMLNHRNVIDELSLTIDEHA 312

[Close]

BLAST of HO062767 vs. TrEMBL
Match: C6TI45_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.017e-20
Identity = 36/61 (59.02%), Postives = 43/61 (70.49%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMD K K +  +         GAIL++RSA+GARAFLYP+VDP  DLKGFE LS+FHP DE
Sbjct:  204 GMDHKEKGRV-ISHLAKFMAPGAILLLRSAHGARAFLYPVVDPSSDLKGFEALSVFHPTDE 263

HSP 2 Score: 51.9878 bits (123), Expect = 4.017e-20
Identity = 30/48 (62.50%), Postives = 36/48 (75.00%), Query Frame = -3

Query:   98 VINSVMVARKCSVNQEVYLKSKCCEVEGFINPLNHGNVIEELSIDEKA 241
            VINSV+VARK   +  V L SKC  VEGF N  NHGNVIEEL++D++A
Sbjct:  264 VINSVIVARKGLYSSPV-LASKCSGVEGF-NHFNHGNVIEELTVDDQA 309

[Close]

BLAST of HO062767 vs. TrEMBL
Match: B9RFJ1_RICCO (Nicotianamine synthase, putative OS=Ricinus communis GN=RCOM_1435160 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.792e-20
Identity = 48/105 (45.71%), Postives = 61/105 (58.10%), Query Frame = -2

Query:  243 AYDLFHPHQDFIKRXXFXTNKHFFYVKKCS*RFIMLCFWACIWFGMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 557
            A  L   H D  KR  F T      V+     F ++   A +  GMDK+ K +  +         GAIL++RSA+GARAFLYP+VDP CDL+GFEVLS+FHP DE
Sbjct:  162 ALRLVSSHPDLSKRMFFHTTD-IMNVENGLQEFDVVFLAALV--GMDKEEKIRV-IDHLAKYMAPGAILMLRSAHGARAFLYPVVDP-CDLRGFEVLSVFHPTDE 261

HSP 2 Score: 50.0618 bits (118), Expect = 6.792e-20
Identity = 28/58 (48.28%), Postives = 42/58 (72.41%), Query Frame = -3

Query:  101 VINSVMVARKC-----SVNQEVY------LKSKCCEVEGFINPLNHGNVIEELSIDEK 241
            VINSV++A+K      S++Q+V+      L SKC E++ F NPLNHGN+IEEL+I+++
Sbjct:  262 VINSVVIAQKHQLPIQSIDQQVFVPNNIILPSKCSEIQAF-NPLNHGNMIEELAIEDQ 318

[Close]

BLAST of HO062767 vs. TrEMBL
Match: C6TI94_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.149e-19
Identity = 38/62 (61.29%), Postives = 51/62 (82.26%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLD-GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+GKN+  +I      ++ GA+L++RSA+GARAFLYP+V+P CDL+GFEVLS+FHP DE
Sbjct:  203 GMDKEGKNR--IIDHLGKHMNPGALLMLRSAHGARAFLYPVVEP-CDLRGFEVLSVFHPSDE 261

HSP 2 Score: 47.7506 bits (112), Expect = 1.149e-19
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = -3

Query:  101 VINSVMVARKC-------SVNQE-----VYLKSKCCEVEGFINPLNHGNVIEELSIDEK 241
            V+NSV++ARK        S++Q+     + L +KC E++ F NPLNHGN+IEEL+++E+
Sbjct:  262 VVNSVVIARKLYHPVPMHSLDQQALVGSMILPNKCSEIQAF-NPLNHGNMIEELAVEEQ 319

[Close]

BLAST of HO062767 vs. TrEMBL
Match: A5B0Y3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020491 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.953e-19
Identity = 31/40 (77.50%), Postives = 38/40 (95.00%), Query Frame = -2

Query:  243 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 362
            GA+L++RSA+GARAFLYP++DP CDL+GFEVLSIFHP DE
Sbjct:  223 GALLMLRSAHGARAFLYPVIDP-CDLRGFEVLSIFHPTDE 261

HSP 2 Score: 40.0466 bits (92), Expect = 2.953e-19
Identity = 30/81 (37.04%), Postives = 43/81 (53.09%), Query Frame = -1

Query:  367 TTNMFHNYDIDP-LXIQSL*FISSTPRLYQKXXFSX**TFFLCEKMFLKIYNVVFLGLHLVWHG*KGKKQKVINHLAKYMA 606
            TT  FHNYDIDP     +   ++S P   ++  F    T  +     LK Y+VV+L   +     K +K +V++HLAKYMA
Sbjct:  145 TTTTFHNYDIDPAANSMAAHLVASDPDFSKRMLFHT--TDIMNVTNELKEYDVVYLAALVGMD--KEEKIQVMDHLAKYMA 221

HSP 3 Score: 30.0314 bits (66), Expect = 2.953e-19
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = -3

Query:  104 VINSVMVARKC-----SVNQ---EVYLKSKCCEVEGFINPLNHGNVIEELSIDE 241
            VINSV+VARK      S++Q    + L SKC E++ F       N +EEL+I+E
Sbjct:  262 VINSVVVARKFPMHVHSLDQGLGPMILPSKCSEIQVF-------NPLEELTIEE 308

[Close]

BLAST of HO062767 vs. TrEMBL
Match: D7UA30_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031240001 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 8.175e-19
Identity = 31/40 (77.50%), Postives = 38/40 (95.00%), Query Frame = -2

Query:  243 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 362
            GA+L++RSA+GARAFLYP++DP CDL+GFEVLSIFHP DE
Sbjct:  371 GALLMLRSAHGARAFLYPVIDP-CDLRGFEVLSIFHPTDE 409

HSP 2 Score: 39.2762 bits (90), Expect = 8.175e-19
Identity = 30/81 (37.04%), Postives = 43/81 (53.09%), Query Frame = -1

Query:  367 TTNMFHNYDIDP-LXIQSL*FISSTPRLYQKXXFSX**TFFLCEKMFLKIYNVVFLGLHLVWHG*KGKKQKVINHLAKYMA 606
            TT  FHNYDIDP     +   +SS P   ++  F    T  +     LK Y+VV+L   +     K +K +V++HLAK+MA
Sbjct:  293 TTTSFHNYDIDPAANSMAAHLVSSDPDFSKRMLFHT--TDIMNVTNELKEYDVVYLAALVGMD--KEEKIQVMDHLAKHMA 369

HSP 3 Score: 29.261 bits (64), Expect = 8.175e-19
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = -3

Query:   98 VINSVMVARKC-----SVNQ---EVYLKSKCCEVEGFINPLNHGNVIEELSIDEKA 241
            VINSV+VARK      S++Q    + L SKC E++ F       N +EEL+I++ +
Sbjct:  410 VINSVVVARKFPMHVHSLDQGLGPMILPSKCSEIQVF-------NPLEELTIEDSS 458

[Close]

BLAST of HO062767 vs. TrEMBL
Match: B9N4R2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_581960 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.553e-18
Identity = 47/106 (44.34%), Postives = 60/106 (56.60%), Query Frame = -2

Query:  243 KAYDLFHPHQDFIKRXXFXTNKHFFYVKKCS*RFIMLCFWACIWFGMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 560
            KA  L   H D   R  F T        +    F ++   A +  GMDK+ K K  +         GAIL++RSA+GARAFLYP++DP CDL+GFEVLS+FHP DE
Sbjct:  161 KALQLVTSHPDLSSRMFFHTTDIMDVTSELQ-EFDVVFLAALV--GMDKEEKVKV-IDHLAKQMAPGAILMLRSAHGARAFLYPVIDP-CDLRGFEVLSVFHPSDE 261

HSP 2 Score: 43.1282 bits (100), Expect = 4.553e-18
Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = -3

Query:  101 VINSVMVARKCS-----VNQE-------VYLKSKCCEVEGFINPLNHGNVIEELSIDEK 241
            VINSV++ARK S     ++Q+       + L +KCCE+  F NPL+H  ++EEL+++E+
Sbjct:  262 VINSVVIARKYSLPINSIDQQQTGLGSMIVLPNKCCEILAF-NPLSHVTMVEELAVEEQ 319

[Close]

BLAST of HO062767 vs. TrEMBL
Match: B9RZP0_RICCO (Nicotianamine synthase, putative OS=Ricinus communis GN=RCOM_1000280 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.949e-16
Identity = 36/61 (59.02%), Postives = 45/61 (73.77%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDKK K +  +         GAIL++RSA+GARAFLYP++DP  DL+GFEVLS+FHP DE
Sbjct:  203 GMDKKEKVQV-IKHLAEHMAPGAILLLRSAHGARAFLYPVIDP-QDLEGFEVLSVFHPTDE 261

HSP 2 Score: 43.5134 bits (101), Expect = 3.949e-16
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = -3

Query:  101 VINSVMVARKC-----SVNQEV---YLKSKCCEVEGFINPLNHGNVIEELSIDEK 241
            VINSV++ARK      S+N  +    L SKC +++GF  P+ H N+IEEL++DE+
Sbjct:  262 VINSVIIARKFLRAVHSLNTGIGSALLPSKCSDIQGF-KPITHTNLIEELTVDEQ 315

[Close]

BLAST of HO062767 vs. TrEMBL
Match: A2Q2X7_MEDTR (Nicotianamine synthase OS=Medicago truncatula GN=MtrDRAFT_AC152185g32v2 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 6.702e-16
Identity = 54/106 (50.94%), Postives = 63/106 (59.43%), Query Frame = -2

Query:  243 KAYDLFHPHQDFIKRXXFXTNKHFFYVKKCS*RFIMLCFWACIWFGMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 560
            KAYDL     D  KR  F T+     VK     F ++   A +  GMDKK K K  +         GAILV+RSA+GA+AFLY +VDP CDLKGFEVLSIFHP DE
Sbjct:  162 KAYDLISKDNDLSKRMLFHTSD-IVDVKNELKEFNVVFLAALV--GMDKKEKAKV-INHLAKYMAPGAILVLRSAHGAKAFLYHVVDPSCDLKGFEVLSIFHPTDE 263

HSP 2 Score: 25.0238 bits (53), Expect = 6.702e-16
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = -3

Query:  194 VINSVMVARKCSVNQE 241
            VINSV+VARK  VNQ+
Sbjct:  264 VINSVIVARKGLVNQQ 279

[Close]

BLAST of HO062767 vs. TAIR peptide
Match: AT1G09240.1 (| Symbols: NAS3, ATNAS3 | nicotianamine synthase 3 | chr1:2984950-2985912 FORWARD LENGTH=320)

HSP 1 Score: 64.3142 bits (155), Expect = 1.483e-16
Identity = 29/40 (72.50%), Postives = 37/40 (92.50%), Query Frame = -2

Query:  243 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 362
            GA+L++RSA+G RAFLYPIV+P CDL+GFEVLSI+HP D+
Sbjct:  226 GAVLMLRSAHGPRAFLYPIVEP-CDLQGFEVLSIYHPTDD 264

HSP 2 Score: 38.891 bits (89), Expect = 1.483e-16
Identity = 31/79 (39.24%), Postives = 44/79 (55.70%), Query Frame = -1

Query:  367 MFHNYDIDPL--XIQSL*FISSTPRLYQKXXFSX**TFFLCEKMFLKIYNVVFLGLHLVWHG*KGKKQKVINHLAKYMA 597
            +FHN+DIDP    + SL  +SS P + Q+  F       + E   LK ++VVFL   +  +  K +K KVI HL K+MA
Sbjct:  151 IFHNFDIDPSANSLASL-LVSSDPDISQRMFFHTVDIMDVTES--LKSFDVVFLAALVGMN--KEEKVKVIEHLQKHMA 224

[Close]

BLAST of HO062767 vs. TAIR peptide
Match: AT1G56430.1 (| Symbols: ATNAS4, NAS4 | nicotianamine synthase 4 | chr1:21137023-21137997 FORWARD LENGTH=324)

HSP 1 Score: 66.2402 bits (160), Expect = 1.503e-11
Identity = 36/61 (59.02%), Postives = 45/61 (73.77%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDKK K K  +         GA+L++RSA+G RAFLYPIV+P CDL+GFEVLS++HP DE
Sbjct:  207 GMDKKEKVKV-VEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP-CDLEGFEVLSVYHPTDE 265

[Close]

BLAST of HO062767 vs. TAIR peptide
Match: AT5G56080.1 (| Symbols: ATNAS2, NAS2 | nicotianamine synthase 2 | chr5:22711402-22712364 REVERSE LENGTH=320)

HSP 1 Score: 63.5438 bits (153), Expect = 9.742e-11
Identity = 34/61 (55.74%), Postives = 44/61 (72.13%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+ K K  +         GA++++RSA+G RAFLYPIVD  CDLKGFEVL+I+HP D+
Sbjct:  206 GMDKESKVKA-IEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD-SCDLKGFEVLTIYHPSDD 264

[Close]

BLAST of HO062767 vs. TAIR peptide
Match: AT5G04950.1 (| Symbols: NAS1, ATNAS1 | nicotianamine synthase 1 | chr5:1457876-1458838 REVERSE LENGTH=320)

HSP 1 Score: 56.6102 bits (135), Expect = 1.191e-8
Identity = 31/61 (50.82%), Postives = 42/61 (68.85%), Query Frame = -2

Query:  243 GMDKKGKNKK*LIIWPNTWLDGAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHPIDE 425
            GMDK+ K K  +         GA+L++RSA+  RAFLYPIVD   DLKGF++L+I+HP D+
Sbjct:  207 GMDKESKVKA-IEHLEKHMAPGAVLMLRSAHALRAFLYPIVD-SSDLKGFQLLTIYHPTDD 265

[Close]

BLAST of HO062767 vs. TAIR peptide
Match: AT4G26483.1 (| Symbols: | nicotianamine synthases | chr4:13376647-13376970 REVERSE LENGTH=84)

HSP 1 Score: 53.9138 bits (128), Expect = 7.719e-8
Identity = 26/37 (70.27%), Postives = 31/37 (83.78%), Query Frame = -2

Query:  252 GAILVVRSAYGARAFLYPIVDPCCDLKGFEVLSIFHP 362
            GA+L++RSA G R+FLY  VDP CDLKGFEVL I+HP
Sbjct:    4 GALLMLRSARGLRSFLYVDVDP-CDLKGFEVLEIYHP 39

The following BLAST results are available for this feature:

BLAST of HO062767 vs. SwissProt
BLAST of HO062767 vs. TrEMBL
BLAST of HO062767 vs. TAIR peptide

BLAST of HO062767 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NAS3_ARATH	1.429e-15	72.50	Nicotianamine synthase 3 OS=Arabidopsis thaliana G...	[more]
NAS_SOLLC	7.250e-14	55.74	Nicotianamine synthase OS=Solanum lycopersicum GN=...	[more]
NAS4_ARATH	1.441e-10	59.02	Probable nicotianamine synthase 4 OS=Arabidopsis t...	[more]
NAS2_ARATH	9.343e-10	55.74	Nicotianamine synthase 2 OS=Arabidopsis thaliana G...	[more]
NAS1_ARATH	1.142e-7	50.82	Nicotianamine synthase 1 OS=Arabidopsis thaliana G...	[more]
NAS1_ORYSJ	3.414e-7	67.44	Nicotianamine synthase 1 OS=Oryza sativa subsp. ja...	[more]
NAS1_ORYSI	3.414e-7	67.44	Nicotianamine synthase 1 OS=Oryza sativa subsp. in...	[more]
NAS2_ORYSJ	4.426e-7	65.12	Nicotianamine synthase 2 OS=Oryza sativa subsp. ja...	[more]
NAS3_HORVU	7.403e-7	66.67	Probable nicotianamine synthase 3 OS=Hordeum vulga...	[more]
NAS2_HORVU	7.403e-7	66.67	Probable nicotianamine synthase 2 OS=Hordeum vulga...	[more]

BLAST of HO062767 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A3DUI9_MALXI	7.132e-23	75.00	Nicotianamine synthase OS=Malus xiaojinensis PE=2 ...	[more]
B9A9Z2_LOTJA	9.440e-23	48.11	Nicotianamine synthase OS=Lotus japonicus GN=LjNAS...	[more]
C6TI45_SOYBN	4.017e-20	59.02	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RFJ1_RICCO	6.792e-20	45.71	Nicotianamine synthase, putative OS=Ricinus commun...	[more]
C6TI94_SOYBN	1.149e-19	61.29	Putative uncharacterized protein OS=Glycine max PE...	[more]
A5B0Y3_VITVI	2.953e-19	77.50	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7UA30_VITVI	8.175e-19	77.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9N4R2_POPTR	4.553e-18	44.34	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RZP0_RICCO	3.949e-16	59.02	Nicotianamine synthase, putative OS=Ricinus commun...	[more]
A2Q2X7_MEDTR	6.702e-16	50.94	Nicotianamine synthase OS=Medicago truncatula GN=M...	[more]

BLAST of HO062767 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G09240.1	1.483e-16	72.50	\| Symbols: NAS3, ATNAS3 \| nicotianamine synthase 3...	[more]
AT1G56430.1	1.503e-11	59.02	\| Symbols: ATNAS4, NAS4 \| nicotianamine synthase 4...	[more]
AT5G56080.1	9.742e-11	55.74	\| Symbols: ATNAS2, NAS2 \| nicotianamine synthase 2...	[more]
AT5G04950.1	1.191e-8	50.82	\| Symbols: NAS1, ATNAS1 \| nicotianamine synthase 1...	[more]
AT4G26483.1	7.719e-8	70.27	\| Symbols: \| nicotianamine synthases \| chr4:13376...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 28..43 score
None	No IPR available	SEG	seg	seg	coord: 12..32 score
None	No IPR available	PFAM	PF03059	NAS	coord: 10..51 score: 3.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062767 ID=HO062767; Name=HO062767; organism=Cicer arietinum; type=EST; length=606bp
AAAAAAAACATTGAATTCATTACGGCAATGTAATCTCATATCACAAATAA
ACATTCTCAACAACAAATTACATGCATTATTAAGAATAAATACATTAAGC
TTTCTCATCAATGCTCAACTCCTCGATAACATTCCCATGATTCAAAGGAT
TAATAAAACCTTCAACTTCACAACACTTGCTTTTCAAGTAAACCTCTTGA
TTCACCGAACACTTCCTTGCAACCATAACCGAATTTATAACCCTCATCAA
TTGGATGAAAAATTGAAAGAACTTCAAAACCTTTAAGATCACAACAAGGA
TCAACAATAGGGTATAGAAAAGCTCTAGCACCATAAGCACTTCTCACAAC
AAGAATAGCACCATCCAGCCATGTATTTGGCCAAATGATTAATCACTTTT
TGTTTTTTCCCTTTTTATCCATGCCAAACCAAATGCAAGCCCAGAAACAC
AACATTATAAATCTTTAAGAACATTTTTTCACATAAAAAAAATGTTTATT
AGTNNGAAAAANNNTTCTTTTGATAAAGTCTTGGTGTGGATGAAATAAAT
CATAAGCTTTGAATTNCCAATGGATCAATGTCGTAGTTGTGAAACATGTT
TGTGGT

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